Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,735,320	C→T	M105I (ATG→ATA)	sucA ←	2‑oxoglutarate dehydrogenase subunit E1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,735,320	0	C	T	100.0%	255.8 / NA	74	M105I (ATG→ATA)	sucA	2‑oxoglutarate dehydrogenase subunit E1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/41);  total (33/41)

CCTCTTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGCATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGATAC  >  NC_002947/4735175‑4735463                                                                                                                                                  |                                                                                                                                                cctcTTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGtata                                                                                                                                              >  5:133928/1‑149 (MQ=255)   tctTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATAc                                                                                                                                             >  7:336688/1‑148 (MQ=255)        gCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAg                                                                                                                                        <  2:67462/148‑1 (MQ=255)           gATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCt                                                                                                                                    >  8:347539/1‑149 (MQ=255)               aaCAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGAt                                                                                                                                >  6:14832/1‑149 (MQ=255)                aCAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGAt                                                                                                                                >  5:72859/1‑148 (MQ=255)                aCAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGAt                                                                                                                                >  3:231820/1‑148 (MQ=255)                   ggTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGt                                                                                                                            >  2:16938/1‑149 (MQ=255)                    gTCGCCGGCACGGAAGGTCGTATCTAGATCGGGATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACGCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGAt                                                                                                                                <  3:270159/144‑1 (MQ=255)                        ccGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAg                                                                                                                       <  6:187944/149‑1 (MQ=255)                                gAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCaa                                                                                                               <  4:64342/149‑1 (MQ=255)                                  aGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAAcc                                                                                                             <  3:319954/149‑1 (MQ=255)                                   ggTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCt                                                                                                            <  1:288972/149‑1 (MQ=255)                                   ggTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCt                                                                                                            <  7:347539/149‑1 (MQ=255)                                       gTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGctt                                                                                                        <  1:16938/149‑1 (MQ=255)                                        tATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGcttc                                                                                                       <  5:57425/149‑1 (MQ=255)                                         aTCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGcttct                                                                                                      >  4:209869/1‑149 (MQ=255)                                         aTCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGcttct                                                                                                      >  6:366209/1‑149 (MQ=255)                                         aTCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGcttct                                                                                                      >  2:118038/1‑149 (MQ=255)                                         aTCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGcttct                                                                                                      >  2:184206/1‑149 (MQ=255)                                           cAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGc                                                                                                                                      >  6:207711/1‑115 (MQ=255)                                              gATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCg                                                                                                 <  6:211399/149‑1 (MQ=255)                                               aTCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCgt                                                                                                <  7:85070/149‑1 (MQ=255)                                                   gCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTc                                                                                            <  4:231820/149‑1 (MQ=255)                                                     aTTGGTCAAGCCGTAGTGATTGATCGACCGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCAc                                                                                          <  8:303110/149‑1 (MQ=255)                                                     aTTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCAc                                                                                          <  3:209869/149‑1 (MQ=255)                                                     aTTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCAc                                                                                          <  5:366209/149‑1 (MQ=255)                                                         gTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCt                                                                                      >  5:32515/1‑149 (MQ=255)                                                          tCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCt                                                                                      >  5:48742/1‑148 (MQ=255)                                                          tCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTc                                                                                     <  6:133928/149‑1 (MQ=255)                                                             aGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACg                                                                                  >  4:103216/1‑149 (MQ=255)                                                                cGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAAcc                                                                                                             >  2:183778/1‑119 (MQ=255)                                                                cGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAAcc                                                                                                             <  1:183778/119‑1 (MQ=255)                                                                  tAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTccc                                                                             >  2:73237/1‑149 (MQ=255)                                                                           atCGACAGGGCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGaaa                                                                     <  6:202344/148‑1 (MQ=255)                                                                             cGACAGGTCTACGAGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCg                                                                  >  2:221479/1‑149 (MQ=255)                                                                             cGACAGGTCTACGAGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCg                                                                  >  2:226863/1‑149 (MQ=255)                                                                               aCAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGt                                                                <  1:184206/149‑1 (MQ=255)                                                                                cAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGtt                                                               <  1:73237/149‑1 (MQ=255)                                                                                cAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGtt                                                               >  6:29540/1‑149 (MQ=255)                                                                                 aGGTCTACGGGCGCAGGGCGCTGACACAACCCCAACGGGTCGAGCTTGGCAGCCTGATAGCCGCGTATACGATAGGCCTGAAACAGACGCAGAACTTCAACCTGCTTATTATCGTGTTCACTGCTCACCCTCCCGGCGGAAACCGGTTg                                                              >  7:56460/1‑149 (MQ=255)                                                                                  ggTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTgg                                                             <  1:50082/149‑1 (MQ=255)                                                                                       aCGGGCGCAGGGCGCTGCCACACCCCGAACGGGTCGCGCTTGGCAGCCTGATGGCCGCGTATAGGATAGGCCTGGATCAGTCGCAGAACTGCAACCTGCTGCGTCTCGTGGTCACTGCGCACGCTCCCGGCGGAAGCCGGGTGggggcg                                                        >  4:238865/1‑149 (MQ=255)                                                                                        cGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGggcgcgg                                                       >  6:30261/1‑149 (MQ=255)                                                                                             gcAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTg                                                  <  6:32515/149‑1 (MQ=255)                                                                                               aGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGtt                                                               <  6:301205/134‑1 (MQ=255)                                                                                               aGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGtt                                                               >  5:301205/1‑134 (MQ=255)                                                                                                 ggCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTc                                              >  2:45935/1‑149 (MQ=255)                                                                                                 ggCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTc                                              >  2:8640/1‑149 (MQ=255)                                                                                                    gcTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCttt                                           <  1:226863/149‑1 (MQ=255)                                                                                                    gcTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCttt                                           <  1:221479/149‑1 (MQ=255)                                                                                                     cTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTg                                          >  2:364550/1‑149 (MQ=255)                                                                                                        ccACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCca                                       <  5:30261/149‑1 (MQ=255)                                                                                                        ccACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCca                                       <  6:72859/149‑1 (MQ=255)                                                                                                          acaACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCcag                                      >  5:115766/1‑148 (MQ=255)                                                                                                           caACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCcagca                                    <  6:48742/149‑1 (MQ=255)                                                                                                           caACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCcagca                                    <  7:97701/149‑1 (MQ=255)                                                                                                            aaCCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCcagcag                                   <  6:180858/149‑1 (MQ=255)                                                                                                             aCCCCACCTGGTCGAGCTTGGCAGGCTGATGGACGCGTATACGATCGGACTGGATCAGTCGGAGTCCTTAAACCTTCTTCTTCTCGTGTTCACTGCTCACGCTCCGGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGt                                  <  5:357186/149‑1 (MQ=255)                                                                                                               cccAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGAGCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTAc                                <  6:314208/149‑1 (MQ=255)                                                                                                                ccAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTc                                                                               <  3:12290/101‑1 (MQ=255)                                                                                                                ccAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTc                                                                               >  4:12290/1‑101 (MQ=255)                                                                                                                ccAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTAc                                >  4:323589/1‑148 (MQ=255)                                                                                                                 cAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACg                               >  7:336185/1‑148 (MQ=255)                                                                                                                 cAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACg                               >  1:176209/1‑148 (MQ=255)                                                                                                                 cAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTCCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGa                              <  1:118038/149‑1 (MQ=255)                                                                                                                        tCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTc                       >  8:46133/1‑149 (MQ=255)                                                                                                                                ggCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGAtt                <  1:45935/148‑1 (MQ=255)                                                                                                                                         aTGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTg        <  3:103216/147‑1 (MQ=255)                                                                                                                                         aTGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGc       <  6:115766/148‑1 (MQ=255)                                                                                                                                          tGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGa     <  4:173409/149‑1 (MQ=255)                                                                                                                                          tGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGa     <  8:336688/149‑1 (MQ=255)                                                                                                                                           ggCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGa           >  2:238604/1‑142 (MQ=255)                                                                                                                                           ggCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGa           <  1:238604/142‑1 (MQ=255)                                                                                                                                             ccGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGATAc  <  1:364550/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                CCTCTTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGCATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGATAC  >  NC_002947/4735175‑4735463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑