Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	353,067	A→G	intergenic (+34/+84)	hisF → / ← cbcV	imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	353,067	0	A	G	100.0%	115.3 / NA	35	intergenic (+34/+84)	hisF/cbcV	imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/16);  total (19/16)

GGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA  >  NC_002947/352925‑353209                                                                                                                                               |                                                                                                                                               ggAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCAGCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCGTCGCGCGGGCTCGTCGGTCGCTGAGCCCTTGACCGGGGCGGCTTTGCGGCCCTTCGCggggccg                                                                                                                                          >  8:69182/1‑145 (MQ=255)  gAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGc                                                                                                                                         >  8:351750/1‑149 (MQ=255)             cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCca                                                                                                                                >  5:25911/1‑147 (MQ=255)               gcgcGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAgg                                                                                                                            <  6:25911/149‑1 (MQ=255)                     tGCTGGTGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                                      >  8:160279/1‑149 (MQ=255)                          gcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTTGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCACCCACAGGTTTGGTGAGAt                                                                                                                 >  2:197759/1‑149 (MQ=39)                                      ttCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCAACCCCGCTCCCACAGGTTTGCTGAGATCACTGTGGGAGc                                                                                                     >  2:1646/1‑149 (MQ=255)                                       tCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCg                                                                                                    >  2:290489/1‑149 (MQ=255)                                         cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCggg                                                                                                  >  5:184288/1‑149 (MQ=255)                                         cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCgg                                                                                                   <  7:10086/148‑1 (MQ=255)                                              tGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCtgtg                                                                                                          <  7:187731/136‑1 (MQ=255)                                              tGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCtgtg                                                                                                          >  8:187731/1‑136 (MQ=255)                                                      acacAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAg                                                                                     >  3:116015/1‑149 (MQ=255)                                                       cacaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAg                                                                                     <  7:351750/148‑1 (MQ=255)                                                        acaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAGGTTTGGGGTGATGGCTGTGGGAGCGGGCGTGCCCGCGAAggg                                                                                   >  8:90919/1‑149 (MQ=38)                                                           gTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCcg                                                                                >  2:78806/1‑149 (MQ=255)                                                                    gCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGACATCTCTGTGGGAGCGGGCGTGGCCGCGAAGGGCCGCGAAGCGGc                                                                       >  5:278482/1‑149 (MQ=255)                                                                     ccAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCTCCCACACGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCCAAGCGCCGCCAAGCGAcc                                                                      >  8:341404/1‑149 (MQ=38)                                                                      cAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTGCGCGGGCACGCCCGCTCCCACCGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGccc                                                                     <  3:112099/149‑1 (MQ=255)                                                                        aGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGCGCTCCTTTGCAGCCCTTCGCGGGCACGCCCCCAACCACACCGATGCAGAGATCTAAGTTGGAGCCGGCGTTCCCGCAAAGCGCCGCGAAGCCGcccc                                                                    >  6:212524/1‑148 (MQ=255)                                                                              aCATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGAGGGCCCCCCCCCCCCCCCACGGTTGGTGGGATCTCTGTGGGGGCGGGCGGGCCCGCCCACGGCCCCGCAGCCGcccccaatggg                                                             >  6:223930/1‑142 (MQ=255)                                                                               cctGGCGTCGCGCGGGCGCGGCGTTCGCTGAGCCATTGCCCGGGGCTGCGTTGCAGCCCGGCGCGGGCACGCCCGCTCCCACCGGTGTGCTGAGATCTCTGGGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCtt                                                            <  7:161216/147‑1 (MQ=25)                                                                               cATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCtt                                                            <  1:1646/149‑1 (MQ=255)                                                                               cATGGCTTCGCGCGGGAGGGTCTTTCGCTGTTCCATTGACTGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTCAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCtt                                                            <  7:341404/149‑1 (MQ=37)                                                                                aTGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGACATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTc                                                           <  7:9214/149‑1 (MQ=255)                                                                                        gcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAAACGTgg                                                   >  1:21367/1‑149 (MQ=255)                                                                                               aTCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGc                                            >  8:264239/1‑149 (MQ=255)                                                                                                 cgtcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGtt                                                 >  6:114916/1‑142 (MQ=255)                                                                                                 cgtcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGtt                                                 <  5:114916/142‑1 (MQ=255)                                                                                                      ttCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagcag                                     <  1:290489/149‑1 (MQ=255)                                                                                                       tCGCTGATCCATTGCCCGGGGCGGCTTTGCAGCCCTTCGTGGGCACGCCCGCTCCCACTGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagcagc                                    <  5:223930/149‑1 (MQ=38)                                                                                                          cTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGcgcg                                 <  2:71305/149‑1 (MQ=255)                                                                                                             aTCCATTGACCGGGGCTGCTTTGCAGCCCTGCGCGGGCACGCCCGCTCCCACCGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTg                               <  1:197759/148‑1 (MQ=255)                                                                                                                 aTTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTaaa                          <  1:78806/149‑1 (MQ=255)                                                                                                                               ctTTGCAGCCCTTCGCGGGCAGGCCCGCTCCCACTGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATcccc             <  6:278482/148‑1 (MQ=255)                                                                                                                                         cTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCcca  >  2:225019/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                               GGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA  >  NC_002947/352925‑353209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑