| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 97.4% | 110.0 / NA | 38 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (15/22); total (15/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 97.4% | 108.2 / NA | 38 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (15/22); minor base G (0/1); total (15/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGT‑GT‑T‑TCGGGCACG‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG > NC_002947/1499341‑1499619 || aCGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTg > 3:351841/1‑149 (MQ=255) ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCg < 4:351841/149‑1 (MQ=255) gCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCgg > 3:276694/1‑149 (MQ=255) cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCTGTGCATGGCACC‑GGCTTTGCCGgt‑g < 5:3577/149‑1 (MQ=255) ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑Gt‑tcgc > 6:338274/1‑146 (MQ=255) tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑Gt‑tcgc < 6:192756/148‑4 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑Gt‑tcgcgg < 5:358474/148‑6 (MQ=255) aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCAcg‑cccgctc < 2:40030/149‑6 (MQ=25) ccGTGATGCGGCCATTCAGACCCTGAACCACGCCTTGGCACCACAGCAGGACTGAGGCTGCCAACCGGTGGGAGCGGGCGTCCCCGCTAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCGGGT‑GT‑TCGCGGCCACGCCC‑GCTCCca < 6:306501/149‑1 (MQ=18) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTACCAAACGGTGGGAGCGGGCGTGCCCGCGAAACACGCGCCCCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TAGCGGGCACGCCC‑GCTCCca > 2:20832/1‑148 (MQ=21) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGAc < 6:186338/148‑1 (MQ=21) cATTCAGAGCCTGAACTAAGCCTTGGCACCACTCCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGc < 8:205677/149‑1 (MQ=21) agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAACGCGCCCCCGTGCATGGCACC‑CGCTTTGCCGGT‑GT‑TAGCGGGCACGCCC‑GCTCCCCCAGACTGATTGCCAta > 4:316877/1‑147 (MQ=21) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGc < 5:169620/149‑1 (MQ=21) acctaaGCCTTGGCACAACCGCAGCACTGTGGCTCCCAACGGGGGGGCGGGGGGGGCCCCGCTAACCAGGCGCCGCGGT‑CCTGGCCCCGGGCTTGGCGGGT‑GTGC‑GCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGc < 2:344247/144‑1 (MQ=255) aagcaaGCCTTGCCACCCCAGCCGGACTGCGGCTGCCAACCGGGGGGTCCGGGCGGGCCCGCTAAACTGGGCCCGGGGTGCATGGCACG‑GGTTTTGCCGGT‑GT‑TCGGGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGc < 2:144009/144‑1 (MQ=255) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGAtt > 5:70945/1‑132 (MQ=18) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGAtt < 6:70945/132‑1 (MQ=18) gCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGc < 1:104908/91‑1 (MQ=255) gCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGc > 2:104908/1‑91 (MQ=255) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACACGAGCCCCGGTGCATGGCACC‑GGGATTGGCCGT‑GT‑TCGCGGGCCCG‑CCAGCAGCCCCAGACAGCTTGCCATTAATCGGGCCGATCGCGACCTTGACg > 2:269692/1‑149 (MQ=12) gAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 4:276694/149‑1 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt > 7:37042/1‑149 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 8:346796/149‑1 (MQ=25) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc > 6:164727/1‑149 (MQ=25) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc > 6:335789/1‑149 (MQ=25) gCCCGCGAAACCGGCGCCGCGGTGCATGCCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 5:294165/149‑1 (MQ=37) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 2:106586/149‑1 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 5:338274/148‑1 (MQ=37) aaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCGCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc < 1:20832/149‑1 (MQ=37) cAGGCGCCGCGGTTCAGGGCAGC‑GGCTTTGCCGGTGGT‑C‑GCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa < 7:246569/149‑1 (MQ=38) gcgcCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 3:162670/1‑148 (MQ=37) gccgcGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 3:316877/149‑1 (MQ=37) ccgcGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 8:46428/127‑1 (MQ=25) ccgcGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa > 7:46428/1‑127 (MQ=25) gCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 4:283689/100‑1 (MQ=21) gCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt > 3:283689/1‑100 (MQ=21) cATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 5:24700/1‑148 (MQ=38) || ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGT‑GT‑T‑TCGGGCACG‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG > NC_002947/1499341‑1499619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |