Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,470,649	Δ1 bp	7.1%	coding (1256/1890 nt)	mrdA‑II ←	transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,470,649	0	A	.	7.1%	264.0 / 13.4	85	coding (1256/1890 nt)	mrdA‑II	transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (36/43);  new base . (3/3);  total (39/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCAGTGGCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGACCCAGCCATCCCCGGA  >  NC_002947/5470503‑5470797                                                                                                                                                    |                                                                                                                                                      gtgcagtgGCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCATACTCCTCGaa                                                                                                                                                      >  6:228735/1‑148 (MQ=255) gtgcagtgGCCT‑GGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACa                                                                                                                                                    >  2:238038/1‑149 (MQ=255)  tgcagtgGCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATAAGGGTTACGCAAGGGAACCAGGCATGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGAATCACCCCGGCAGACTCAACGAAc                                                                                                                                                     >  8:213471/1‑148 (MQ=255)     agtgGCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑t                                                                                                                                                >  4:283933/1‑149 (MQ=255)       tgGCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCGAATGCGGAGAATCAGGGTTTCGCCAGGGAACCAGGCGTGGCGACGGGTGGCCCGCTGCCATTCGCGCGACGGCATCAACCGGGCAGCCTCCTCGAACATG‑Tc                                                                                                                                               >  1:80243/1‑149 (MQ=255)         gCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAg                                                                                                                                            <  7:197588/149‑1 (MQ=255)         gCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAg                                                                                                                                            <  5:67096/149‑1 (MQ=255)         gCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAg                                                                                                                                            >  8:197588/1‑149 (MQ=255)          ccTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAgg                                                                                                                                           <  2:243606/149‑1 (MQ=255)          ccTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCG‑ACATG‑ttcgag                                                                                                                                           >  4:204431/1‑143 (MQ=255)           cTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCG‑ACATGTTCGAgga                                                                                                                                          <  6:222707/149‑2 (MQ=255)               gCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCG‑ACATGTTCGAggagtct                                                                                                                                      <  3:204431/149‑6 (MQ=255)                ccAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAAcc                                                                                                                                     >  1:188505/1‑149 (MQ=255)                ccAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCG‑ACATGTTCGAggag                                                                                                                                         <  1:199107/145‑3 (MQ=255)                ccAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCG‑ACATGTTCGAggag                                                                                                                                         >  2:199107/1‑143 (MQ=255)                    ttGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCt                                                                                                                                 >  7:147186/1‑149 (MQ=255)                    ttGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCt                                                                                                                                 <  4:183535/149‑1 (MQ=255)                     tGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTg                                                                                                                                >  6:295834/1‑149 (MQ=255)                            gcgTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATa                                                                                                                         <  3:283933/149‑1 (MQ=255)                              gTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATAcc                                                                                                                       >  3:170018/1‑149 (MQ=255)                               tGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACAt                                                                                                                                                   <  4:299696/121‑1 (MQ=255)                               tGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACAt                                                                                                                                                   >  3:299696/1‑121 (MQ=255)                                 gCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGaa                                                                                                                    >  7:250724/1‑149 (MQ=255)                                    tGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGTTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACtt                                                                                                                 >  6:131282/1‑149 (MQ=255)                                      cATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACtt                                                                                                                 <  6:94267/147‑1 (MQ=255)                                        tataGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTc                                                                                                             <  2:80243/149‑1 (MQ=255)                                        tataGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTc                                                                                                             <  7:213471/149‑1 (MQ=255)                                          taGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCATACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCAt                                                                                                           <  5:228735/149‑1 (MQ=255)                                          taGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCAt                                                                                                           <  1:238038/149‑1 (MQ=255)                                            gCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATg                                                                                                          <  8:13332/148‑1 (MQ=255)                                            gCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGt                                                                                                         <  8:250724/149‑1 (MQ=255)                                             cccTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATg                                                                                                          <  2:10114/147‑1 (MQ=255)                                             cccTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATg                                                                                                          >  1:10114/1‑147 (MQ=255)                                              ccTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAg                                                                                                       >  6:42991/1‑149 (MQ=255)                                               cTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGc                                                                                                      <  5:170587/149‑1 (MQ=255)                                                    cG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGa                                                                                                  >  5:179129/1‑148 (MQ=255)                                                        tGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAg                                                                                              <  3:124171/149‑1 (MQ=255)                                                         gCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGTCACGCTTCCATTCGCGCGACTGCATCAACCCGGCAGACTCCTCGAACATC‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAg                                                                                              <  4:273929/148‑1 (MQ=255)                                                           cGAGAATCAGGGTTTCGCCAGTGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcga                                                                                           <  6:93258/149‑1 (MQ=255)                                                           cGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCt                                                                                                                                 <  7:80781/111‑1 (MQ=255)                                                           cGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCt                                                                                                                                 >  8:80781/1‑111 (MQ=255)                                                           cGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcg                                                                                            >  8:117860/1‑148 (MQ=255)                                                            gagaATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcgat                                                                                          <  6:179129/149‑1 (MQ=255)                                                            gagaATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcgat                                                                                          <  5:227576/149‑1 (MQ=255)                                                            gagaATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcgat                                                                                          >  6:147518/1‑149 (MQ=255)                                                                aTCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcgatcgat                                                                                      <  7:166630/149‑1 (MQ=255)                                                                aTCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcgatcgat                                                                                      <  8:201823/149‑1 (MQ=255)                                                                 tCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCAt                                                                                                           <  1:156736/127‑1 (MQ=255)                                                                 tCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCAt                                                                                                           >  2:156736/1‑127 (MQ=255)                                                                 tCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATg                                                                                     >  4:244193/1‑149 (MQ=255)                                                                   aGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGc                                                                                    <  6:332127/148‑1 (MQ=255)                                                                     ggTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCa                                                                                 >  2:180525/1‑149 (MQ=255)                                                                     ggTTTCGCCAGGGAACAAGGCCTGGCGACGGGTGGCACGCTTCCATTAGCGCGACGGCATCAACCCGGCAGACTCCTCGAACCTG‑TCGAGGGAAACCCGCTGGCCGAAACCGAACTTGTTAATGTAGCTGGACAGGCGATCGATGCCCa                                                                                 >  8:140552/1‑149 (MQ=255)                                                                          cGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCtt                                                                             <  3:244193/148‑1 (MQ=255)                                                                             cAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTgc                                                                         <  5:42991/149‑1 (MQ=255)                                                                              aGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGACCTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTAGTgcg                                                                        >  2:330786/1‑149 (MQ=255)                                                                                 gAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCaa                                                                     <  1:180525/149‑1 (MQ=255)                                                                                    ccAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAgg                                                                   >  8:227868/1‑148 (MQ=255)                                                                                      aGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCa                                                                >  6:354748/1‑149 (MQ=255)                                                                                            ggCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAg                                                          >  6:277401/1‑149 (MQ=255)                                                                                             gCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAAGATG‑TGCTGGGAAAGCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCTAGGTCATAGAAGt                                                         <  7:40256/149‑1 (MQ=255)                                                                                             gCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGt                                                         <  6:238736/149‑1 (MQ=255)                                                                                                    gTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTc                                                  >  2:75423/1‑149 (MQ=255)                                                                                                    gTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGATGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTc                                                  >  5:104248/1‑149 (MQ=255)                                                                                                       gCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGtt                                               >  8:82497/1‑149 (MQ=255)                                                                                                       gCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGt                                                <  8:147186/148‑1 (MQ=255)                                                                                                           gCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGATCATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAg                                           <  7:82497/149‑1 (MQ=255)                                                                                                            cTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAgc                                          <  1:75423/149‑1 (MQ=255)                                                                                                            cTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAgc                                          <  5:354748/149‑1 (MQ=255)                                                                                                                cATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCa                                       <  6:231123/148‑1 (MQ=255)                                                                                                                 aTTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAg                                                       >  8:74409/1‑131 (MQ=255)                                                                                                                 aTTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAg                                                       <  7:74409/131‑1 (MQ=255)                                                                                                                  ttCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGc                                                                                             >  5:200246/1‑92 (MQ=255)                                                                                                                  ttCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGc                                                                                             <  6:200246/92‑1 (MQ=255)                                                                                                                     gcgcgACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCatgatg                                  <  7:117860/148‑1 (MQ=255)                                                                                                                       gcgACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCg                               <  2:188505/149‑1 (MQ=255)                                                                                                                            ggCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTc                          >  3:104018/1‑149 (MQ=255)                                                                                                                             gCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCGGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTcc                         <  5:295834/149‑1 (MQ=255)                                                                                                                               aTCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAg                       >  5:320662/1‑149 (MQ=255)                                                                                                                                     ccGGCAGACTCCTCGAACATG‑TCGAGGGAAA‑CCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGAcc                >  8:10200/1‑149 (MQ=255)                                                                                                                                        gCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGACCCa              <  6:104248/149‑1 (MQ=255)                                                                                                                                           tttccctcG‑ACATGTTCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGACCCAGc            >  7:82837/5‑148 (MQ=255)                                                                                                                                                ctcGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAg                                                       >  2:286009/1‑100 (MQ=255)                                                                                                                                                ctcGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAg                                                       <  1:286009/100‑1 (MQ=255)                                                                                                                                                 tcGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGt                                                         <  2:334374/97‑1 (MQ=255)                                                                                                                                                 tcGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGt                                                         >  1:334374/1‑97 (MQ=255)                                                                                                                                                    aaCATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGACCCAGCCATCCCCGGa  <  4:170018/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GTGCAGTGGCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGACCCAGCCATCCCCGGA  >  NC_002947/5470503‑5470797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑