Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 482,860 Δ1 bp 7.3% coding (1579/1923 nt) yeaG ← protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947482,8600T. 7.3% 169.7 / 7.2 55coding (1579/1923 nt)yeaGprotein kinase
Reads supporting (aligned to +/- strand):  ref base T (25/26);  new base . (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATAGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGC  >  NC_002947/482718‑483000
                                                                                                                                              |                                                                                                                                            
tCATAGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGt                                                                                                                                         <  2:62783/148‑1 (MQ=255)
 cATAGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGtt                                                                                                                                        <  8:188731/148‑1 (MQ=255)
 cATAGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGtt                                                                                                                                        <  1:11950/148‑1 (MQ=255)
 cATAGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGtt                                                                                                                                        <  3:19251/148‑1 (MQ=255)
  aTAGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTga                                                                                                                                      <  2:232402/149‑1 (MQ=255)
    aGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAA‑GCGGt                                                                                                                                         >  8:98358/1‑143 (MQ=255)
    aGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAA‑GCGGt                                                                                                                                         <  7:98358/143‑1 (MQ=255)
    aGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAA‑GCGGt                                                                                                                                         <  7:93593/143‑1 (MQ=255)
    aGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAA‑GCGGt                                                                                                                                         >  8:93593/1‑143 (MQ=255)
                         tGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAACTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTcg                                                                                                               >  8:284828/1‑149 (MQ=255)
                          gCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGCTTGCTGCTCCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTcgc                                                                                                              >  5:247274/1‑149 (MQ=255)
                            cATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCgg                                                                                                            >  3:59691/1‑149 (MQ=255)
                                gttgttgGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTAtt                                                                                                        >  3:53225/1‑149 (MQ=255)
                                    actgcgcgcACGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCt                                                                                                     >  6:120982/4‑148 (MQ=255)
                                       gcgcGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCgg                                                                                                                   >  2:225400/1‑131 (MQ=255)
                                       gcgcGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCgg                                                                                                                   <  1:225400/131‑1 (MQ=255)
                                            tGCGCGCAGTACGAAGTTGACGATCTCCTGCCGGAAGACTTTCGGGCTGCAGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGa                                                                                            <  6:207448/149‑1 (MQ=255)
                                              cgcgcAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATc                                                                                          <  4:53225/149‑1 (MQ=255)
                                                cgcAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCa                                                                                        <  3:5525/149‑1 (MQ=255)
                                                     tACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGCGTTCTCGATCTTCTCCAGTTACTCGATGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCCGTATTCCTGATCCTGCATCCACaaa                                                                                    >  1:89318/1‑148 (MQ=255)
                                                      aCGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATc                                                                                  >  7:140825/1‑149 (MQ=255)
                                                       cGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCt                                                                                 >  1:333289/1‑149 (MQ=255)
                                                       cGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGAGCCTGGATCCAGAAATCt                                                                                 >  1:328215/1‑149 (MQ=255)
                                                        gAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTg                                                                                <  7:284828/149‑1 (MQ=255)
                                                          aGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCa                                                                              <  4:59691/149‑1 (MQ=255)
                                                           gTTGACGATCTCGGTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCAt                                                                             <  2:60320/149‑1 (MQ=255)
                                                            ttGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATa                                                                            >  3:45082/1‑149 (MQ=255)
                                                                       gTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAg                                                                  <  6:247274/148‑1 (MQ=255)
                                                                                   tttCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGt                                                     >  7:270437/1‑149 (MQ=255)
                                                                                             cTGATGCCAGCCGGGTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGt                                            <  4:45082/148‑1 (MQ=255)
                                                                                              tGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGc                                               >  4:102324/1‑144 (MQ=255)
                                                                                              tGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGc                                               <  3:102324/144‑1 (MQ=255)
                                                                                                     aGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTcgcg                                                                                                             >  7:243912/1‑75 (MQ=255)
                                                                                                     aGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTcgcg                                                                                                             <  8:243912/75‑1 (MQ=255)
                                                                                                      gCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGcc                                              <  4:88543/137‑1 (MQ=255)
                                                                                                      gCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGcc                                              >  3:88543/1‑137 (MQ=255)
                                                                                                          gTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTgg                                                <  5:286424/131‑1 (MQ=255)
                                                                                                          gTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTgg                                                >  6:286424/1‑131 (MQ=255)
                                                                                                          gTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGAc                              >  2:144957/1‑149 (MQ=255)
                                                                                                              ctcGATCTTCTACCGTTCCTCGTTGAGCGCAATGCGGTTGAGAAATTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCTTACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTcc                           <  8:140825/148‑1 (MQ=255)
                                                                                                               tcGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAAttt                                                                                                                                >  8:68287/1‑46 (MQ=255)
                                                                                                               tcGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAAttt                                                                                                                                <  7:68287/46‑1 (MQ=255)
                                                                                                               tcGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAg                         >  1:345743/1‑149 (MQ=255)
                                                                                                                 gATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAgg                        >  2:29754/1‑148 (MQ=255)
                                                                                                                   tcttctCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGTAg                     >  7:121607/1‑149 (MQ=255)
                                                                                                                       ctcCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGTAgg                    <  8:270437/146‑1 (MQ=255)
                                                                                                                       caccAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTATCCGGGTCGCGGTATTGCTGATCCAGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGACGTATTCGCTGAAGGACTCCAGGTAGCCAg                 >  6:105332/3‑149 (MQ=255)
                                                                                                                        tcCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGt                       <  5:1265/142‑1 (MQ=255)
                                                                                                                        tcCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGt                       >  6:1265/1‑142 (MQ=255)
                                                                                                                                 tCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTc       >  8:86090/1‑149 (MQ=255)
                                                                                                                                    ttGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTgg            <  6:154022/141‑1 (MQ=255)
                                                                                                                                    ttGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTgg            >  5:154022/1‑141 (MQ=255)
                                                                                                                                    ttGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCtt    <  1:144957/149‑1 (MQ=255)
                                                                                                                                       aGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGGCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGc  <  2:333289/148‑1 (MQ=255)
                                                                                                                                       aGCGCAATGCGGTTGACAATTTCGCCGGCTTCCGGGTCGCGGTATTCCCGCTCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGc  <  2:328215/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
TCATAGCTCAGCCAGCTTGGATTCTTGCCATTGTTGTTGGCGCGTGCGCGCAGTACGAAGTTGACGATCTCGTTGCGGAAGTCTTTCGGGTTGCTGATGCCAGCCGGTTTCTCGATCTTCTCCAGTTCCTCGTTGAGCGCAATGCGGTTGAGAATTTCGCCGGTTTCCGGGTCGCGGTATTCCTGATCCTGGATCCAGAAATCTGCATACAGCACGTAGCGGTCGAAGATGTTCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGC  >  NC_002947/482718‑483000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑