Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	698,287	Δ1 bp	6.2%	noncoding (464/1522 nt)	PP_16SD →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	698,287	0	T	.	6.2%	177.4 / 7.0	64	noncoding (464/1522 nt)	PP_16SD	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (33/27);  new base . (2/2);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

CGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAAGCCCCGGGC  >  NC_002947/698147‑698420                                                                                                                                             |                                                                                                                                      cGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGa                                                                                                                                 >  4:325946/1‑147 (MQ=32)    gAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGaa                                                                                                                            >  1:42988/1‑149 (MQ=32)    gAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGaa                                                                                                                            >  5:265655/1‑149 (MQ=32)     aGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGaa                                                                                                                            >  7:32472/1‑148 (MQ=32)                gggAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCt                                                                                                                <  4:187344/149‑1 (MQ=32)                  gAATATTCGACATTGGGTGAAATCCTGATCCAGACATGCCGCGTGTGTGAAGATCGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGATTAAGCACCGGGTaa                                                                                                              <  8:148791/149‑1 (MQ=11)                         ggACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCtgt                                                                                                        <  2:36232/148‑1 (MQ=32)                          taCAATGGGCTAAAGCATGATCCATCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACATAATAAGCACCGGCTAACTCtgtg                                                                                                       <  8:262009/147‑1 (MQ=255)                          gACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGc                                                                                                      >  5:5140/1‑149 (MQ=32)                               tGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGGCGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCcag                                                                                                   >  4:283905/1‑147 (MQ=17)                                 ggCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCcagcag                                                                                                <  2:42988/148‑1 (MQ=32)                                 ggCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCcagcag                                                                                                <  6:265655/148‑1 (MQ=32)                                    gAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCcgc                                                                                            >  8:113001/1‑149 (MQ=32)                                     aaaGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCcgcg                                                                                           >  5:66623/1‑149 (MQ=32)                                          cTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAAt                                                                                      <  7:323733/149‑1 (MQ=32)                                             aTCCAGCCATGCCGCGTGTGTGAAGAAGGTCGTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATAc                                                                                    <  3:192695/148‑1 (MQ=17)                                               ccAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACG‑TACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACagag                                                                                >  7:15838/1‑149 (MQ=17)                                               ccAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACG‑TACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACagag                                                                                >  7:10948/1‑149 (MQ=17)                                                   ccATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATaa                                                                                                                         <  4:10891/105‑1 (MQ=32)                                                   ccATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATaa                                                                                                                         >  3:10891/1‑105 (MQ=32)                                                                 tgAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCcagca                                                                                                 <  7:71783/115‑1 (MQ=32)                                                                 tgAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCcagca                                                                                                 >  8:71783/1‑115 (MQ=32)                                                                   aagaagGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCgg                                                              <  3:321091/148‑1 (MQ=32)                                                                   aagaagGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCgg                                                              >  4:321091/1‑148 (MQ=32)                                                                   aagaagGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACG‑TACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGa                                                             <  8:10948/148‑1 (MQ=17)                                                                   aagaagGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACG‑TACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGa                                                             <  8:15838/148‑1 (MQ=17)                                                                      aagGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTTCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAAt                                                           >  3:33187/1‑148 (MQ=17)                                                                      aagGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGATAACTCTGTGCCAGCAGCCGAGGTAATACAGAGGGTGCAAGCGTTAATCGGAAt                                                           >  1:92351/1‑148 (MQ=17)                                                                            ttCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTCCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTggg                                                    >  5:218784/1‑149 (MQ=17)                                                                              cGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTgg                                                     <  1:209692/146‑1 (MQ=32)                                                                                gATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCaa                                                                         >  2:123071/1‑124 (MQ=32)                                                                                     tAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCATCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAgcg                                           >  2:112288/1‑149 (MQ=17)                                                                                     tAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAgcg                                           >  7:156960/1‑149 (MQ=32)                                                                                     tAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAgcg                                           >  5:269661/1‑149 (MQ=32)                                                                                       aaGCACTTTAAGTTGGGAGGAAGGGCATTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATc                                                                <  1:298264/126‑1 (MQ=17)                                                                                       aaGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATc                                                                >  2:298264/1‑126 (MQ=32)                                                                                       aaGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAgcgc                                          <  4:33187/148‑1 (MQ=32)                                                                                           aCTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTa                                     <  3:325946/149‑1 (MQ=32)                                                                                             tttAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACagag                                                                                <  1:64091/104‑1 (MQ=32)                                                                                             tttAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACagag                                                                                >  2:64091/1‑104 (MQ=32)                                                                                             tttAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATc                                                                >  1:65191/1‑120 (MQ=32)                                                                                             tttAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATc                                                                <  2:65191/120‑1 (MQ=32)                                                                                             tttAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAgg                                   >  3:225471/1‑149 (MQ=32)                                                                                             tttAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGAAGCCGATGTAATACAGATGGTGCAAGCGTTAATCGGAATTACTGGGCTTAAATCGCGCGTAgg                                   >  7:290726/1‑149 (MQ=11)                                                                                               tAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAggt                                  <  8:156960/148‑1 (MQ=32)                                                                                                 aGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGCCGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAggtgg                                <  3:283905/148‑1 (MQ=17)                                                                                                  gTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGtt                              <  6:66623/149‑1 (MQ=32)                                                                                                   ttGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGttt                             <  8:32472/149‑1 (MQ=32)                                                                                                     gggAGGAAGCGCAGTAAGTTAATACATTGCTCTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTCTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGt                           >  4:281573/1‑149 (MQ=255)                                                                                                      ggaggaAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGt                           <  7:113001/148‑1 (MQ=32)                                                                                                      ggaggaAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGt                           <  6:5140/148‑1 (MQ=32)                                                                                                       gaggaAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTa                                                <  4:112646/126‑1 (MQ=32)                                                                                                       gaggaAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTa                                                >  3:112646/1‑126 (MQ=32)                                                                                                        aggaAGGCCAGTAAGTTAATACCTAGCTGTCTTGACGTAACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCCTTAATCGGAATTACTGGGCGTAAAGCCCCCGTAGCTCGTTTGta                          >  4:85923/1‑146 (MQ=11)                                                                                                          gaAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGt                      >  6:300424/1‑149 (MQ=32)                                                                                                             gggCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTg                    >  6:181540/1‑148 (MQ=32)                                                                                                             gggCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTg                    >  7:296122/1‑148 (MQ=32)                                                                                                               gCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATAc                                                                                    <  5:323905/82‑1 (MQ=33)                                                                                                               gCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATAc                                                                                    >  6:323905/1‑82 (MQ=33)                                                                                                               gCACTAAGTTAATACCTTGCTGGCTTGACGTTACCGACAGAATAAGCCCCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGAt                 <  4:149748/149‑1 (MQ=12)                                                                                                                           tACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACCGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAGGCGCGCGTAGGGGGTTTGTGAAGTTGGATGTGACAGCCCCg     >  4:97775/1‑149 (MQ=11)                                                                                                                             ccTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAAGCCCCggg   >  6:265054/1‑149 (MQ=32)                                                                                                                             ccTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAAGCCCCggg   <  5:300424/149‑1 (MQ=32)                                                                                                                              cTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAAGCCCCGGGc  >  7:227500/1‑149 (MQ=32)                                                                                                                                             |                                                                                                                                      CGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTCTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAAGCCCCGGGC  >  NC_002947/698147‑698420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑