Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,030,808	Δ1 bp	5.3%	coding (866/1353 nt)	PP_0887 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,030,808	0	A	.	5.3%	238.4 / 6.7	75	coding (866/1353 nt)	PP_0887	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (37/34);  new base . (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

CCGGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGG  >  NC_002947/1030664‑1030954                                                                                                                                                   |                                                                                                                                                   ccgGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAg                                                                                                                                                <  7:5203/149‑1 (MQ=255)  cgGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGc                                                                                                                                               <  4:201462/149‑1 (MQ=255)    gTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGTCTCTTTGC‑ACAGCttt                                                                                                                                            <  3:188237/149‑3 (MQ=255)     tGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTGTT‑GCAAAGTCTCTTTGC‑ACAGCttt                                                                                                                                            <  7:124118/149‑3 (MQ=255)        cgccCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTgc                                                                                                                                         <  4:131388/149‑1 (MQ=255)          cccATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTgcgc                                                                                                                                       >  8:85446/1‑149 (MQ=255)          cccATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTgcgc                                                                                                                                       <  3:310230/149‑1 (MQ=255)            cATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGTCTCTTTGCa                                                                                                                                                    >  1:197181/1‑134 (MQ=255)            cATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGTCTCTTTGCa                                                                                                                                                    <  2:197181/134‑1 (MQ=255)             aTGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCa                                                                                                                                    <  4:67741/149‑1 (MQ=255)              tGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTGTT‑GCAAAGTCTCTTTGCaca                                                                                                                                                  <  1:207552/135‑3 (MQ=255)              tGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTGTT‑GCAAAGTCTCTTTGCaca                                                                                                                                                  >  2:207552/1‑133 (MQ=255)               gAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGc                                                                                                                                  >  5:73981/1‑149 (MQ=255)                  cTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCg                                                                                                                                >  1:241916/1‑148 (MQ=255)                     ggTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGc                                                                                                                            >  2:248251/1‑149 (MQ=255)                       tACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGcc                                                                                                                           >  2:247642/1‑148 (MQ=255)                          gccgcTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAAcc                                                                                                                       >  1:322359/1‑149 (MQ=255)                              cTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGgcg                                                                                                                   <  4:221975/149‑1 (MQ=255)                                 gCGACCATGAGCGTGCTGATAAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCt                                                                                                                >  1:306630/1‑149 (MQ=255)                                    aCCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGc                                                                                                             <  6:73981/149‑1 (MQ=255)                                      cATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCa                                                                                                           <  2:74982/149‑1 (MQ=255)                                           gCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTg                                                                                                      <  1:248251/149‑1 (MQ=255)                                            cGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTgg                                                                                                     <  3:235467/149‑1 (MQ=255)                                              tGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAg                                                                                                   <  7:85446/149‑1 (MQ=255)                                                 tGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACa                                                                                                                                                 >  4:32323/1‑100 (MQ=255)                                                 tGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACa                                                                                                                                                 <  3:32323/100‑1 (MQ=255)                                                    tCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAt                                                                                             >  7:16135/1‑149 (MQ=255)                                                      aaCGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAtgt                                                                                           >  8:74540/1‑149 (MQ=255)                                                      aaCGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAtgt                                                                                           >  3:110562/1‑149 (MQ=255)                                                       aCGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAtgtg                                                                                          <  4:304081/149‑1 (MQ=255)                                                               cgcCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCctg                                                                                  <  4:110562/149‑1 (MQ=255)                                                                gcCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCctgg                                                                                 >  5:118774/1‑149 (MQ=255)                                                                 ccAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCctggc                                                                                <  2:306630/149‑1 (MQ=255)                                                                     gACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAt                                                                                             >  4:61274/1‑132 (MQ=255)                                                                     gACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAt                                                                                             <  3:61274/132‑1 (MQ=255)                                                                      aCCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGAc                                                                           >  6:28179/1‑149 (MQ=255)                                                                        cacaCCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGAc                                                                           >  6:297167/1‑147 (MQ=255)                                                                        cacaCCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGa                                                                         <  2:241916/149‑1 (MQ=255)                                                                          cacCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAgg                                                                       <  2:664/149‑1 (MQ=255)                                                                           acCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAgg                                                                       >  1:267500/1‑149 (MQ=255)                                                                           acCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCACGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGgc                                                                      >  7:287672/1‑149 (MQ=255)                                                                                  cGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAAc                                                               >  2:18837/1‑149 (MQ=255)                                                                                  cGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTCGCCTTCGTGGAGCAGGATCTGACCGCCTGGCTGGACGAGGCGCTCAAc                                                               >  8:289073/1‑149 (MQ=255)                                                                                   gCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAAcc                                                              >  8:192059/1‑149 (MQ=255)                                                                                   gCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAAcc                                                              <  1:247642/149‑1 (MQ=255)                                                                                         gCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTgg                                                         >  1:222274/1‑148 (MQ=255)                                                                                           aggaggACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCAc                                                      >  3:211566/1‑149 (MQ=255)                                                                                            ggaggaCCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCAcc                                                     >  5:236741/1‑149 (MQ=255)                                                                                              aggaCCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTg                                                   <  7:192059/149‑1 (MQ=255)                                                                                                gaCCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGAt                                                 <  5:169634/149‑1 (MQ=255)                                                                                                 aCCTGCAAATACTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGAt                                                 <  8:16135/148‑1 (MQ=255)                                                                                                   cTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATgcg                                              <  4:211566/149‑1 (MQ=255)                                                                                                     gCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAAc                                                                                                                        <  4:265299/73‑1 (MQ=255)                                                                                                     gCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAAc                                                                                                                        >  3:265299/1‑73 (MQ=255)                                                                                                      cAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGccc                                           <  1:7902/149‑1 (MQ=255)                                                                                                       aaaTCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCg                                          >  2:82612/1‑149 (MQ=255)                                                                                                          tcctccAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCgg                                         >  6:269320/1‑147 (MQ=255)                                                                                                          tcctccAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGaa                                       <  7:74540/149‑1 (MQ=255)                                                                                                             tccAGTACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGc                                     >  2:267107/1‑148 (MQ=255)                                                                                                             tccAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGc                                     >  6:328428/1‑148 (MQ=255)                                                                                                               cAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCa                                   <  5:328428/148‑1 (MQ=255)                                                                                                                  gACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTa                               >  2:268809/1‑149 (MQ=255)                                                                                                                  gACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTa                               <  2:222274/149‑1 (MQ=255)                                                                                                                  gACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTCATGCGCCCGGAAGCCAGcaa                               >  2:276786/1‑147 (MQ=255)                                                                                                                         tAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTc                        <  5:269320/149‑1 (MQ=255)                                                                                                                            aGCTGTT‑GCAAAGTCTCTTTGC‑ACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAg                     >  1:251342/1‑149 (MQ=255)                                                                                                                             gCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCACAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAgc                    >  8:300111/1‑149 (MQ=255)                                                                                                                              cTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAgcg                   >  6:332532/1‑149 (MQ=255)                                                                                                                               tgtt‑gCAAAGTCTCTTTGC‑ACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAgcgc                  >  4:273445/4‑149 (MQ=255)                                                                                                                                     cAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTgc              <  4:59715/149‑1 (MQ=255)                                                                                                                                         gagaCTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGa          >  6:78044/1‑149 (MQ=255)                                                                                                                                           gaCTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACg        <  2:267500/149‑1 (MQ=255)                                                                                                                                            aCTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACgg       >  7:210288/1‑149 (MQ=255)                                                                                                                                             cTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGc      <  5:28179/149‑1 (MQ=255)                                                                                                                                              tttGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGc      >  4:279534/1‑148 (MQ=255)                                                                                                                                              tttGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGc      >  2:198548/1‑148 (MQ=255)                                                                                                                                                tGCAACAGCTGGTGCACGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCaa    <  8:287672/148‑1 (MQ=255)                                                                                                                                                 gCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAgg  >  6:169969/1‑149 (MQ=255)                                                                                                                                                 gCAACAGCTGGTGCGCACAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAg   <  7:300111/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CCGGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGG  >  NC_002947/1030664‑1030954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑