Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,558,481	Δ1 bp	5.3%	coding (420/732 nt)	PP_1369 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,558,481	0	C	.	5.3%	246.5 / 6.4	75	coding (420/732 nt)	PP_1369	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (38/33);  new base . (2/2);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCGCACAGCGTGCGGGACAGTTTGCGACGCCATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGAACTCGCCTTCATGG  >  NC_002947/1558342‑1558628                                                                                                                                            |                                                                                                                                                     aGTCGCACAGCGTGCGGGACAGTTTGCGACGCCATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGc                                                                                                                                            >  4:20324/1‑149 (MQ=255)  gTCGCACAGCGTGCGGGACAGTTTGCGACGCCATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCACGCGGCA‑AGCGCtgtgcc                                                                                                                                           >  2:343076/1‑144 (MQ=255)   tCGCACAGCGTGCGGGACAGTTTGCGACGCCATGCGCTCCTGGCGCTCCAGACCAGGCCTGGCGCATCAATCGACGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCcg                                                                                                                                          >  6:295334/1‑149 (MQ=255)       acaGCGTGCGGGACAGTTTGCGACGCCATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTg                                                                                                                                      >  1:209036/1‑149 (MQ=255)        caGCGTGCGGGACAGTTTGCGACGCCATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCACGCGGCA‑AGCGCTGTGCCGCCTGc                                                                                                                                     <  1:343076/149‑1 (MQ=255)                  gACAGTTTGCGACGCCATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCAt                                                                                                                           >  1:160696/1‑149 (MQ=255)                       tttGCGACGCCATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGc                                                                                                                       >  3:77010/1‑148 (MQ=255)                       tttGCGACGCCATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGc                                                                                                                       >  5:340590/1‑148 (MQ=255)                            gACGCCATGCGCTCCGGGCGCTCCTGACCAGGGCTGGCGCATCAATCGCCGTAAAGTCGGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGccc                                                                                                                                   <  3:318238/131‑1 (MQ=255)                             aCGCCATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCg                                                                                                                >  2:253491/1‑149 (MQ=255)                                 cATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGc                                                                                                            >  5:77246/1‑149 (MQ=255)                                   tGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGa                                                                                                          >  1:47652/1‑149 (MQ=255)                                      gcTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGc                                                                                                                                            <  2:293608/112‑1 (MQ=255)                                      gcTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGc                                                                                                                                            >  1:293608/1‑112 (MQ=255)                                        tCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAAc                                                                                                     >  5:313344/1‑149 (MQ=255)                                            ggCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTg                                                                                                 >  5:312202/1‑149 (MQ=255)                                                 tCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCca                                                                                              >  8:288646/1‑147 (MQ=255)                                                 tCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCca                                                                                              >  1:62580/1‑147 (MQ=255)                                                 tCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCca                                                                                              >  5:216036/1‑147 (MQ=255)                                                   cAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAgg                                                                                          >  2:138810/1‑149 (MQ=255)                                                   cAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAgg                                                                                          <  2:209036/149‑1 (MQ=255)                                                            ccTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTg                                                                                 <  3:299215/149‑1 (MQ=255)                                                             cTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTgg                                                                                >  7:116794/1‑149 (MQ=255)                                                                gcgcATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTc                                                                              <  6:312202/148‑1 (MQ=255)                                                                  gcATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTc                                                                           <  6:340590/149‑1 (MQ=255)                                                                   cATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTg                                                                                 >  2:201816/1‑142 (MQ=255)                                                                   cATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTg                                                                                 <  1:201816/142‑1 (MQ=255)                                                                   cATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGACGTGTACTGTGGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCg                                                                          <  5:295334/149‑1 (MQ=255)                                                                       aaTCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTcc                                                                             <  2:17844/142‑1 (MQ=255)                                                                       aaTCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTcc                                                                             >  1:17844/1‑142 (MQ=255)                                                                           gCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTg                                                                                 <  7:183417/134‑1 (MQ=255)                                                                           gCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTg                                                                                 >  8:183417/1‑134 (MQ=255)                                                                              gTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAg                                                               <  3:329550/149‑1 (MQ=255)                                                                               tAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCCCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAgg                                                              >  5:166458/1‑149 (MQ=255)                                                                                   gTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCcct                                                          >  8:152343/1‑149 (MQ=255)                                                                                   gTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCcct                                                          >  8:282179/1‑149 (MQ=255)                                                                                    tCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCcctc                                                         >  4:87689/1‑149 (MQ=255)                                                                                    tCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCcctc                                                         >  6:163265/1‑149 (MQ=255)                                                                                    tCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCcctc                                                         <  5:200699/149‑1 (MQ=255)                                                                                        gCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGt                                                     <  1:138810/149‑1 (MQ=255)                                                                                          aaGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAg                                                   >  8:304124/1‑149 (MQ=255)                                                                                              ggTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTTGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCgg                                               >  5:146302/1‑149 (MQ=255)                                                                                               gTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCt                                                                                   <  2:200658/112‑1 (MQ=255)                                                                                               gTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCt                                                                                   >  1:200658/1‑112 (MQ=255)                                                                                                tACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGt                                             <  1:253491/149‑1 (MQ=255)                                                                                                  cAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTaa                                           <  5:163265/149‑1 (MQ=255)                                                                                                   aGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGa                                                                                                                         >  7:8491/1‑70 (MQ=255)                                                                                                   aGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGa                                                                                                                         <  8:8491/70‑1 (MQ=255)                                                                                                   aGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAg                                          >  5:231492/1‑149 (MQ=255)                                                                                                     aTGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCcctc                                                         <  5:216008/132‑1 (MQ=255)                                                                                                     aTGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCcctc                                                         >  6:216008/1‑132 (MQ=255)                                                                                                      tGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAgcgc                                       >  2:149360/1‑149 (MQ=255)                                                                                                        cTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTTGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCtt                                     <  6:146302/149‑1 (MQ=255)                                                                                                         tGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGACAGTCCATAATGTAGCAGGGTAAgatattc                                    >  8:169568/1‑143 (MQ=255)                                                                                                           aGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACATCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCt                                                                                   <  3:88625/100‑1 (MQ=255)                                                                                                           aGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCt                                                                                   >  4:88625/1‑100 (MQ=255)                                                                                                            ggTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGtt                                 <  4:309435/149‑1 (MQ=255)                                                                                                            ggTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGtt                                 <  6:216036/149‑1 (MQ=255)                                                                                                             gtgtACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTtc                                <  2:160696/149‑1 (MQ=255)                                                                                                                tACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTtctc                              >  7:106846/1‑148 (MQ=255)                                                                                                                 aCTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCtca                             <  8:106846/148‑1 (MQ=255)                                                                                                                     gtgtATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAATCCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCaa                         <  2:47652/148‑1 (MQ=255)                                                                                                                      tgtATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAgg                       <  7:152343/149‑1 (MQ=255)                                                                                                                          tttCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGt                   <  3:20324/149‑1 (MQ=255)                                                                                                                             cAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGCACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAg                 >  8:287526/1‑148 (MQ=255)                                                                                                                              aGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGaa               <  1:149360/149‑1 (MQ=255)                                                                                                                               gTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGAAc              <  2:62580/149‑1 (MQ=255)                                                                                                                                   cAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCCGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGacggcgc          >  5:178674/1‑143 (MQ=255)                                                                                                                                   cAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGAACTCGc          <  3:34520/149‑1 (MQ=255)                                                                                                                                   cAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGAACTCGc          <  4:77010/149‑1 (MQ=255)                                                                                                                                    acgcggcA‑AGCGCTGTGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAgg                       >  2:180868/3‑135 (MQ=255)                                                                                                                                    acgcggcA‑AGCGCTGTGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAgg                       <  1:180868/133‑1 (MQ=255)                                                                                                                                     ggcggcACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGAACTCGCCt        >  6:194546/1‑149 (MQ=255)                                                                                                                                         gcACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGAACTCGCCTTCAt    <  7:304124/149‑1 (MQ=255)                                                                                                                                         gcACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGAACTCGCCTTCAt    <  7:169568/149‑1 (MQ=255)                                                                                                                                           acaGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGAACTCGCCTTCATgg  >  8:65858/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                                     AGTCGCACAGCGTGCGGGACAGTTTGCGACGCCATGCGCTCCGGGCGCTCCAGACCAGGCCTGGCGCATCAATCGCCGTAAAGTCTGGCAAGCGGTACAGATGCTGAGGTGTACTGGTGTATTTCAGTGGCAGGCGGCACAGCGCT‑TGCCGCGTGCCCTGGCTCATGAGCCAGGCCGGTGCGACAAACCCTGCCACAGGCCACCCTTGGTCCTCGAACAGTGCCAGGCCCTCCTGTAGCCGGGTAAGCGCTTCGTTCTCATCAAGGCTGTAGAACTCGCCTTCATGG  >  NC_002947/1558342‑1558628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑