Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,782,990:1	+A	7.0%	coding (81/771 nt)	nikD →	nickel ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,782,990	1	.	A	7.0%	223.0 / 11.6	57	coding (81/771 nt)	nikD	nickel ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (25/28);  new base A (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

TGATGGCCTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAACTGCTGCTCGACGGCCAGCCGCTGGCTGCAA  >  NC_002947/3782842‑3783135                                                                                                                                                      |                                                                                                                                                   tGATGGCCTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAg                                                                                                                                                     <  7:13958/149‑1 (MQ=255) tGATGGCCTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAg                                                                                                                                                     <  7:19016/149‑1 (MQ=255)        cTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGcgcc                                                                                                                                             >  7:304070/1‑149 (MQ=255)             aCCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCgcggc                                                                                                                                        >  4:52045/1‑149 (MQ=255)                  ctgGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCTCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGaa                                                                                                                                     <  4:256910/147‑1 (MQ=255)                           gcgctgcgcGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGtt                                                                                                                          >  3:4162/1‑149 (MQ=255)                             gctgcgcGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTgg                                                                                                                        >  6:249766/1‑149 (MQ=255)                             gctgcgcGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTgg                                                                                                                        >  8:183297/1‑149 (MQ=255)                              ctgcgcGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGACTTCGCCGCGGCGCAG‑CTGCaag                                                                                                                            >  5:313323/1‑142 (MQ=255)                              ctgcgcGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGACTTCGCCGCGGCGCAG‑CTGCaag                                                                                                                            <  6:313323/144‑3 (MQ=255)                                       cGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAg                                                                                                              <  8:166880/149‑1 (MQ=255)                                       cGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAg                                                                                                              >  7:166880/1‑149 (MQ=255)                                         ccTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCg                                                                                                            >  2:254949/1‑149 (MQ=255)                                          cTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCAGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCgg                                                                                                           >  7:33145/1‑149 (MQ=255)                                              aCCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGctct                                                                                                       <  4:4162/149‑1 (MQ=255)                                               cccTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTg                                                                                                      <  8:304070/149‑1 (MQ=255)                                                    cacaGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGAGCCGCTGGCa                                                                                                   <  5:249766/147‑1 (MQ=255)                                                      caGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATc                                                                                               <  7:99770/149‑1 (MQ=255)                                                       aGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATcc                                                                                              <  8:33145/149‑1 (MQ=255)                                                         gAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCt                                                                                            >  3:122964/1‑149 (MQ=255)                                                         gAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCt                                                                                            >  5:209446/1‑149 (MQ=255)                                                         gAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCt                                                                                            >  7:275302/1‑149 (MQ=255)                                                         gAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCt                                                                                            <  1:250507/149‑1 (MQ=255)                                                               cATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTg                                                                                      >  4:46175/1‑149 (MQ=255)                                                                   gAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCtt                                                                                  >  2:35536/1‑149 (MQ=255)                                                                    aaTGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTg                                                                                                                  >  1:186098/1‑116 (MQ=255)                                                                    aaTGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTg                                                                                                                  <  2:186098/116‑1 (MQ=255)                                                                    aaTGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCtt                                                                                  <  1:35536/148‑1 (MQ=255)                                                                    aaTGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTg                                                                                 >  4:251086/1‑149 (MQ=255)                                                                          tCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGc                                                                           >  1:127545/1‑149 (MQ=255)                                                                            cGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCt                                                                          <  7:183297/148‑1 (MQ=255)                                                                            cGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTc                                                                         <  4:122964/149‑1 (MQ=255)                                                                                 gTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCt                                                                    <  8:275302/149‑1 (MQ=255)                                                                                    cTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGACTTCGCCGCGGCGCAG‑CTGCAAGt                                                                                                                           >  6:149102/1‑91 (MQ=37)                                                                                    cTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGACTTCGCCGCGGCGCAG‑CTGCAAGt                                                                                                                           <  5:149102/91‑1 (MQ=37)                                                                                      tGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTg                                                                                 <  6:198315/131‑1 (MQ=255)                                                                                      tGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTg                                                                                 >  5:198315/1‑131 (MQ=255)                                                                                       gAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCa                                                                                          >  6:205499/1‑121 (MQ=255)                                                                                       gAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCa                                                                                          <  5:205499/121‑1 (MQ=255)                                                                                       gAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTac                                                               >  2:4013/1‑148 (MQ=255)                                                                                       gAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTac                                                               >  7:164205/1‑148 (MQ=255)                                                                                         aaTCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTaccac                                                            <  3:46175/149‑1 (MQ=255)                                                                                           tCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCt                                                          <  5:18736/149‑1 (MQ=255)                                                                                                ggCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCt                                                     >  4:13760/1‑149 (MQ=255)                                                                                                        cATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCa                                            <  2:127545/149‑1 (MQ=255)                                                                                                              aaccgcggCCGTCGTTCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCAGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCGTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCaa                                       <  8:164205/142‑1 (MQ=255)                                                                                                                cccAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGa                                    <  3:52045/149‑1 (MQ=255)                                                                                                                       cGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAActgc                             <  3:251086/149‑1 (MQ=255)                                                                                                                       cGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAActgc                             >  8:308892/1‑149 (MQ=255)                                                                                                                        ggCGTGCGGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCGGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAActgct                            <  8:140104/149‑1 (MQ=255)                                                                                                                          cGTGCTTGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCCGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAACtgctgc                          <  7:217280/149‑1 (MQ=255)                                                                                                                              cTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCCGCACCCAAGGACAACTGCTGCTc                        <  1:4013/148‑1 (MQ=255)                                                                                                                              cTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAACTGCTGCTCGa                      <  7:308892/149‑1 (MQ=255)                                                                                                                               tGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAACTGCTGCTCGAc                     >  2:170083/1‑149 (MQ=255)                                                                                                                                 gTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAACTGCTGCTCGACg                    <  6:209446/148‑1 (MQ=255)                                                                                                                                         cGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCAc                                           <  2:303879/117‑1 (MQ=255)                                                                                                                                         cGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCAc                                           >  1:303879/1‑117 (MQ=255)                                                                                                                                               cTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAACTGCTGCTCGACGGCCAGCCgctggctg     >  6:121872/1‑149 (MQ=255)                                                                                                                                                   cAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAACTGCTGCTCGACGGCCAGCCGCTGGCTGCaa  <  3:13760/148‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   TGATGGCCTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAG‑CTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCG‑CCCGCACCCAAGGACAACTGCTGCTCGACGGCCAGCCGCTGGCTGCAA  >  NC_002947/3782842‑3783135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑