Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 43,461:1 +A 8.9% intergenic (+27/+66) PP_0040 → / ← cadA‑I hypothetical protein/cadmium translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294743,4611.A 8.9% 162.1 / 12.1 45intergenic (+27/+66)PP_0040/cadA‑Ihypothetical protein/cadmium translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base . (19/22);  new base A (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

AGTCGGTTTCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCGCAACAGCG  >  NC_002947/43314‑43608
                                                                                                                                                    |                                                                                                                                                    
aGTCGGTTTCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATc‑g                                                                                                                                                     >  7:81094/1‑149 (MQ=255)
           tAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCg                                                                                                                                          <  8:22441/149‑1 (MQ=255)
                 gtgtCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGtacttc                                                                                                                                    >  6:307842/1‑149 (MQ=255)
                    tCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGtacttctac                                                                                                                                 <  8:81094/149‑1 (MQ=255)
                      aTGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCGGGTA‑GCGCCCGACCCGgacatgtact                                                                                                                                >  8:117175/1‑148 (MQ=255)
                           ccTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGC‑GATCAGCGCACGa                                                                                                                                              <  8:94004/129‑1 (MQ=255)
                           ccTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGC‑GATCAGCGCACGa                                                                                                                                              >  7:94004/1‑129 (MQ=255)
                             tCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTAGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGCGTCGTGCGCTAAGC‑GCGCACGCACCGAACTTCTAAATCAGGTCa                                                                                                                        >  4:257607/1‑149 (MQ=255)
                              cAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCTACGAGTGAGAGAAAAGTACGGGTCGTGCCCTGAAA‑GCGCACGACCCCGACATCTAATTAAGGTAAt                                                                                                                       >  1:320615/1‑149 (MQ=255)
                              cAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCGGATC‑GCGCACGACCCGTACTTCTAGTTGAGGTCAt                                                                                                                       >  6:114676/1‑149 (MQ=255)
                                  gCTGCATTGGCCTGCGGCTTACGGGATGTCTCACCTGAGTGCGGAAATGACCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑TCGCACGACCCGTACTTCTACTTCAGGTCATTTcc                                                                                                                   <  8:243962/149‑1 (MQ=255)
                                            ccTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGtacttctactt                                                                                                                               >  2:265320/1‑127 (MQ=255)
                                            ccTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGtacttctactt                                                                                                                               <  1:265320/127‑1 (MQ=255)
                                                        ggCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCCCGACCCGTACATCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAg                                                                                            >  5:258255/1‑149 (MQ=255)
                                                         gCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGc                                                                                           <  5:307842/149‑1 (MQ=255)
                                                                 cACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGa                                                                                    >  8:83486/1‑148 (MQ=255)
                                                                   ccTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCa                                                                                 <  7:117175/149‑1 (MQ=255)
                                                                    cTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAg                                                                                <  7:83486/149‑1 (MQ=255)
                                                                             gAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATA‑GCGCACGACCCGTACTTATACTTCAGGTCATTTCCCTGCTTAGCTTA‑ATGATTTAAGCTCAGGGATCAGGTGCTTATg                                                                       >  6:257568/1‑149 (MQ=255)
                                                                                    ccTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAgg                                                                <  3:205567/149‑1 (MQ=255)
                                                                                    ccTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATA‑GCGCACGCCCCGTACATATAATTCAGGTGAATTACCTGCTTATCTTA‑ATGATTTAAGCTAAGGGATGAGGTGCGTCTTCGGTatg                                                                >  6:298838/1‑147 (MQ=255)
                                                                                         gaaGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAAc                                                           <  4:126493/148‑1 (MQ=255)
                                                                                            gCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCa                                                                                 >  5:319410/1‑124 (MQ=255)
                                                                                            gCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCa                                                                                 <  6:319410/124‑1 (MQ=255)
                                                                                               tCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGgcg                                                      >  2:234338/1‑148 (MQ=255)
                                                                                                 aaGCGCATCGGGAGCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCaa                                                   <  3:34745/149‑1 (MQ=255)
                                                                                                   gcgcATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATA‑GCGCACGAACCGTACTTCTAATTCAGGTGATTTACCTGCTTAGCTTG‑ATGATTGAAGCTAAAGGATCAGGTGCTTCTGCGGTATGCCAACAAGCGCaatg                                                 >  2:223877/1‑147 (MQ=255)
                                                                                                       aTCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGtt                                             <  5:298838/149‑1 (MQ=255)
                                                                                                        tCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTg                                            <  1:164217/149‑1 (MQ=255)
                                                                                                          gggATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGaa                                          <  1:234338/149‑1 (MQ=255)
                                                                                                                 acgaGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGc                                                                      >  6:340125/1‑114 (MQ=255)
                                                                                                                 acgaGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGc                                                                      <  5:340125/114‑1 (MQ=255)
                                                                                                                      tGAGAGAAAAGTACGGGTCGTGGGCGGATA‑GCGCCCGACCCCGAAGTATACTTGAGGTGATTTACCAGCTTAGCTTG‑ATGATTGAAGATCAGGGAGAAGGGGGGTGTGGGGGAGGGCAAACGGCGCAAGGCGGTGAAGACGAccca                                 >  8:5903/1‑144 (MQ=255)
                                                                                                                      tGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACa                               <  7:5903/148‑1 (MQ=255)
                                                                                                                        agagaAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAgt                             <  6:258255/148‑1 (MQ=255)
                                                                                                                           gaAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCGGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCATCAGtgtg                          <  3:257607/148‑1 (MQ=255)
                                                                                                                                   cGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGAATGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCa                 <  4:241737/149‑1 (MQ=255)
                                                                                                                                     ggTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCTCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATg               <  7:257517/149‑1 (MQ=255)
                                                                                                                                       tCGTGCGC‑GATCAGCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGa                        >  4:300662/1‑138 (MQ=255)
                                                                                                                                       tCGTGCGC‑GATCAGCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGa                        <  3:300662/138‑1 (MQ=255)
                                                                                                                                          tGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCg          <  3:286773/149‑1 (MQ=255)
                                                                                                                                               tGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCGATGATTGAAGCTCAGGGATAAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACAAACAGTGTGGAGCCCTCATGGATCGaaa       >  1:85992/1‑148 (MQ=255)
                                                                                                                                               tGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGtt                                             <  4:124074/109‑1 (MQ=255)
                                                                                                                                               tGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGtt                                             >  3:124074/1‑109 (MQ=255)
                                                                                                                                               tGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCGCaa       >  6:183941/1‑147 (MQ=255)
                                                                                                                                                  tC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCTTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCGCAACAGCg  >  7:75314/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGTCGGTTTCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTC‑ATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCGCAACAGCG  >  NC_002947/43314‑43608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑