Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,984,742:1	+T	7.3%	intergenic (‑43/+10)	cheR ← / ← PP_4393	chemotaxis protein methyltransferase/chemotaxis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,984,742	1	.	T	7.3%	212.2 / 11.6	56	intergenic (‑43/+10)	cheR/PP_4393	chemotaxis protein methyltransferase/chemotaxis protein
Reads supporting (aligned to +/- strand):  ref base . (28/23);  new base T (2/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

GAAACCAGGTACTGCTTATTCTCGCCCAGCAGGATGCCACAGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGAAGTATGCAGG  >  NC_002947/4984605‑4984884                                                                                                                                           |                                                                                                                                               gAAACCAGGTACTGCTTATTCTCGCCCAGCAGGATGCCACAGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCtt                                                                                                                                      >  6:177268/1‑148 (MQ=255)  aaaCCAGGTACTGCTTATTCTCGCCCAGCAGGATGCCACAGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCa                                                                                                                                    <  7:102992/149‑1 (MQ=255)    aCCAGGTACTGCTTATTCTCGCCCAGCAGGATGCCACAGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAAATACCCGTACACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAAt                                                                                                                                  <  6:103147/149‑1 (MQ=255)              gCTTATTCTCGCCCAGCAGGATGCCACAGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCtt                                                                                                                        >  2:289676/1‑149 (MQ=255)               cTTATTCTCGCCCAGCAGGATGCCACAGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTg                                                                                                                       <  8:103135/149‑1 (MQ=255)                      tcGCCCAGCAGGATGCCACAGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGG‑GGGCATGCCCTCCGgca                                                                                                                                          >  5:269133/1‑122 (MQ=255)                      tcGCCCAGCAGGATGCCACAGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGG‑GGGCATGCCCTCCGgca                                                                                                                                          <  6:269133/123‑2 (MQ=255)                        gcacagcagGATGCCACAGGCTTTCTCCAGGAAGACCCTGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCgg                                                                                                                 <  7:149843/143‑1 (MQ=255)                          ccAGCAGGATGCCACAGGCTTTCTCCA‑GAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGac                                                                                                            >  4:110238/1‑148 (MQ=255)                                       acaGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTgg                                                                                                  >  4:223573/1‑146 (MQ=255)                                       acaGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTgg                                                                                                  <  3:223573/146‑1 (MQ=255)                                       acaGGCTTTCTCCAGGAAGACACGGAAGTGTTCGAAATCCAAATTAACCGTAGAAACTACTGCCGACTCTTTTCAAGGACTGGTGCCGGAGGGCATGCCC‑CCGGCTACTTCAATGGGCTGCCTTGATCCGTTCGACCACCCGCTGGGc                                                                                                >  2:49698/1‑148 (MQ=255)                                         aGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAg                                                                                             >  5:11585/1‑149 (MQ=255)                                         aGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAg                                                                                             <  3:298085/149‑1 (MQ=255)                                                 tcCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCg                                                                                     >  3:330836/1‑149 (MQ=255)                                                   cAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGc                                                                                   >  7:229198/1‑149 (MQ=255)                                                   cAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGc                                                                                   <  3:286243/149‑1 (MQ=255)                                                         gACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAAc                                                                             <  1:289676/149‑1 (MQ=255)                                                                        gAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCa                                                              >  8:311807/1‑149 (MQ=255)                                                                         aaaTCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGACGTCAt                                                             <  7:311807/149‑1 (MQ=255)                                                                           aTCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCg                                                           >  6:119325/1‑149 (MQ=255)                                                                            tCCAAATTACCCGTAGACACTACTGCAGCCTCTTTTCAATCACTGGTTCCGGATGGCATGCCC‑CCGTCTGCTTCAATGCGCTGCCTTGATACgg                                                                                                                 <  7:145097/94‑1 (MQ=255)                                                                              cAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCa                                                        >  2:232911/1‑149 (MQ=255)                                                                               aaaTTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCAc                                                       <  6:11585/149‑1 (MQ=255)                                                                                      ccGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATc                                                            >  5:212336/1‑137 (MQ=255)                                                                                      ccGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATc                                                            <  6:212336/137‑1 (MQ=255)                                                                                        gTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACcttct                                              >  6:6576/1‑149 (MQ=255)                                                                                         tAGACACTACTGCCGCCTCGTTTAAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGGCTACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACcttctt                                             <  4:105141/149‑1 (MQ=255)                                                                                             cacTACTGCCGCCTATTTTCAATCACTGTTGCCGGAGGGCATGCCC‑CCCGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATACGGCTTTAACTTCGCCAGGAAGTCATCGGCACCGACCTTCTTGAc                                          >  6:104179/1‑148 (MQ=255)                                                                                              actactGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCa                                        >  7:88080/1‑149 (MQ=255)                                                                                              actactGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCAGCTTGAACTTGGCCAGGaa                                                                  >  4:241871/1‑123 (MQ=255)                                                                                              actactGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCAGCTTGAACTTGGCCAGGaa                                                                  <  3:241871/123‑1 (MQ=255)                                                                                                tactGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCAtt                                      >  2:13183/1‑149 (MQ=255)                                                                                                      cgccTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTgg                                <  1:232911/149‑1 (MQ=255)                                                                                                        ccTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCAt                                                             >  1:67586/1‑118 (MQ=255)                                                                                                        ccTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCAt                                                             <  2:67586/118‑1 (MQ=255)                                                                                                         ctctTTTCAATCACTGGTGCCGGAGGGCATGCCCGCCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGtt                              >  1:220738/1‑149 (MQ=255)                                                                                                         ctctTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGtt                              >  5:170149/1‑148 (MQ=255)                                                                                                         ctctTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGtt                              <  2:220738/148‑1 (MQ=255)                                                                                                          tctTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTg                             >  6:207120/1‑148 (MQ=255)                                                                                                             tttCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAaca                         >  4:64984/1‑149 (MQ=255)                                                                                                                 aaTCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGaa                           <  8:219738/143‑1 (MQ=255)                                                                                                                 aaTCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGaa                           >  7:219738/1‑143 (MQ=255)                                                                                                                   tCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCg                    >  5:233842/1‑148 (MQ=255)                                                                                                                         gTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGc              >  8:63936/1‑148 (MQ=255)                                                                                                                          cagccgggggCATGCCCTCCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGc              >  3:321367/8‑148 (MQ=255)                                                                                                                            ccGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGaa           <  5:207120/148‑1 (MQ=255)                                                                                                                            ccGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGaa           <  5:177268/148‑1 (MQ=255)                                                                                                                            ccGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGaa           <  1:13183/148‑1 (MQ=255)                                                                                                                            ccGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGAAg          <  3:110238/149‑1 (MQ=255)                                                                                                                             cGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGAAg          >  5:335894/1‑148 (MQ=255)                                                                                                                             cGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGAAg          >  4:100052/1‑148 (MQ=255)                                                                                                                                 gggCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGAAGTATgc     <  4:330836/149‑1 (MQ=255)                                                                                                                                  ggCATGCCCTCCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGAAGTATgc     <  4:321367/149‑1 (MQ=255)                                                                                                                                  ggCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGAAGTATg      >  3:233882/1‑147 (MQ=255)                                                                                                                                       gCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGAAGTATgcagg  >  4:224271/1‑146 (MQ=255)                                                                                                                                          c‑ccGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGaa                 <  6:38201/128‑1 (MQ=255)                                                                                                                                          c‑ccGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGaa                 >  5:38201/1‑128 (MQ=255)                                                                                                                                           |                                                                                                                                               GAAACCAGGTACTGCTTATTCTCGCCCAGCAGGATGCCACAGGCTTTCTCCAGGAAGACCCGGAACTGTTCGAAATCCAAATTACCCGTAGACACTACTGCCGCCTCTTTTCAATCACTGGTGCCGGAGGGCATGCCC‑CCGGCTGCTTCAATGCGCTGCCTTGATCCGGTCGACCACCCGCTGGGCCAGGTCATCCGGCTTGAACTTGGCCAGGAAGTCATCGGCACCGACCTTCTTGACCATTGCCTGGTTGAACACCCCGGAAAGCGAAGTATGCAGG  >  NC_002947/4984605‑4984884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑