Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,470,654:1	+T	6.2%	coding (1251/1890 nt)	mrdA‑II ←	transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,470,654	1	.	T	6.2%	322.7 / 13.7	80	coding (1251/1890 nt)	mrdA‑II	transpeptidase
Reads supporting (aligned to +/- strand):  ref base . (32/43);  new base T (2/3);  total (34/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

AGTGGCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGACCCAGCCATCCCCGGA  >  NC_002947/5470507‑5470797                                                                                                                                                      |                                                                                                                                                agtgGCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑t                                                                                                                                                >  4:283933/1‑149 (MQ=255)   tgGCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCGAATGCGGAGAATCAGGGTTTCGCCAGGGAACCAGGCGTGGCGACGGGTGGCCCGCTGCCATTCGCGCGACGGCATCAACCGGGCAGCCTCCTCGAACATG‑Tc                                                                                                                                               >  1:80243/1‑149 (MQ=255)     gCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAg                                                                                                                                            <  7:197588/149‑1 (MQ=255)     gCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAg                                                                                                                                            <  5:67096/149‑1 (MQ=255)     gCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAg                                                                                                                                            >  8:197588/1‑149 (MQ=255)      ccTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAgg                                                                                                                                           <  2:243606/149‑1 (MQ=255)      ccTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCG‑ACATG‑ttcgag                                                                                                                                           >  4:204431/1‑143 (MQ=255)       cTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCG‑ACATGTTCGAgga                                                                                                                                          <  6:222707/149‑2 (MQ=255)           gCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCG‑ACATGTTCGAggagtct                                                                                                                                      <  3:204431/149‑6 (MQ=255)            ccAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAAcc                                                                                                                                     >  1:188505/1‑149 (MQ=255)            ccAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCG‑ACATGTTCGAggag                                                                                                                                         <  1:199107/145‑3 (MQ=255)            ccAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCG‑ACATGTTCGAggag                                                                                                                                         >  2:199107/1‑143 (MQ=255)                ttGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCt                                                                                                                                 >  7:147186/1‑149 (MQ=255)                ttGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCt                                                                                                                                 <  4:183535/149‑1 (MQ=255)                 tGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTg                                                                                                                                >  6:295834/1‑149 (MQ=255)                        gcgTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATa                                                                                                                         <  3:283933/149‑1 (MQ=255)                          gTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATAcc                                                                                                                       >  3:170018/1‑149 (MQ=255)                             gCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGaa                                                                                                                    >  7:250724/1‑149 (MQ=255)                                tGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGTTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACtt                                                                                                                 >  6:131282/1‑149 (MQ=255)                                  cATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACtt                                                                                                                 <  6:94267/147‑1 (MQ=255)                                    tataGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTc                                                                                                             <  7:213471/149‑1 (MQ=255)                                    tataGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTc                                                                                                             <  2:80243/149‑1 (MQ=255)                                      taGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCATACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCAt                                                                                                           <  5:228735/149‑1 (MQ=255)                                      taGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCAt                                                                                                           <  1:238038/149‑1 (MQ=255)                                        gCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATg                                                                                                          <  8:13332/148‑1 (MQ=255)                                        gCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGt                                                                                                         <  8:250724/149‑1 (MQ=255)                                         cccTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATg                                                                                                          <  2:10114/147‑1 (MQ=255)                                         cccTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATg                                                                                                          >  1:10114/1‑147 (MQ=255)                                          ccTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAg                                                                                                       >  6:42991/1‑149 (MQ=255)                                           cTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGc                                                                                                      <  5:170587/149‑1 (MQ=255)                                                cG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGa                                                                                                  >  5:179129/1‑148 (MQ=255)                                                    tGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAg                                                                                              <  3:124171/149‑1 (MQ=255)                                                     gCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGTCACGCTTCCATTCGCGCGACTGCATCAACCCGGCAGACTCCTCGAACATC‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAg                                                                                              <  4:273929/148‑1 (MQ=255)                                                       cGAGAATCAGGGTTTCGCCAGTGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcga                                                                                           <  6:93258/149‑1 (MQ=255)                                                       cGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCt                                                                                                                                 >  8:80781/1‑111 (MQ=255)                                                       cGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCt                                                                                                                                 <  7:80781/111‑1 (MQ=255)                                                       cGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcg                                                                                            >  8:117860/1‑148 (MQ=255)                                                        gagaATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcgat                                                                                          >  6:147518/1‑149 (MQ=255)                                                        gagaATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcgat                                                                                          <  6:179129/149‑1 (MQ=255)                                                        gagaATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcgat                                                                                          <  5:227576/149‑1 (MQ=255)                                                            aTCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcgatcgat                                                                                      <  7:166630/149‑1 (MQ=255)                                                            aTCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGcgatcgat                                                                                      <  8:201823/149‑1 (MQ=255)                                                             tCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCAt                                                                                                           <  1:156736/127‑1 (MQ=255)                                                             tCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCAt                                                                                                           >  2:156736/1‑127 (MQ=255)                                                             tCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATg                                                                                     >  4:244193/1‑149 (MQ=255)                                                               aGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGc                                                                                    <  6:332127/148‑1 (MQ=255)                                                                 ggTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCa                                                                                 >  2:180525/1‑149 (MQ=255)                                                                 ggTTTCGCCAGGGAACAAGGCCTGGCGACGGGTGGCACGCTTCCATTAGCGCGACGGCATCAACCCGGCAGACTCCTCGAACCTG‑TCGAGGGAAACCCGCTGGCCGAAACCGAACTTGTTAATGTAGCTGGACAGGCGATCGATGCCCa                                                                                 >  8:140552/1‑149 (MQ=255)                                                                      cGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCtt                                                                             <  3:244193/148‑1 (MQ=255)                                                                         cAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTgc                                                                         <  5:42991/149‑1 (MQ=255)                                                                          aGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGACCTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTAGTgcg                                                                        >  2:330786/1‑149 (MQ=255)                                                                             gAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCaa                                                                     <  1:180525/149‑1 (MQ=255)                                                                                ccAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAgg                                                                   >  8:227868/1‑148 (MQ=255)                                                                                  aGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCa                                                                >  6:354748/1‑149 (MQ=255)                                                                                        ggCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAg                                                          >  6:277401/1‑149 (MQ=255)                                                                                         gCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAAGATG‑TGCTGGGAAAGCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCTAGGTCATAGAAGt                                                         <  7:40256/149‑1 (MQ=255)                                                                                         gCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGt                                                         <  6:238736/149‑1 (MQ=255)                                                                                                gTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTc                                                  >  2:75423/1‑149 (MQ=255)                                                                                                gTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGATGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTc                                                  >  5:104248/1‑149 (MQ=255)                                                                                                   gCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGtt                                               >  8:82497/1‑149 (MQ=255)                                                                                                   gCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGt                                                <  8:147186/148‑1 (MQ=255)                                                                                                       gCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGATCATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAg                                           <  7:82497/149‑1 (MQ=255)                                                                                                        cTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAgc                                          <  1:75423/149‑1 (MQ=255)                                                                                                        cTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAgc                                          <  5:354748/149‑1 (MQ=255)                                                                                                            cATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCa                                       <  6:231123/148‑1 (MQ=255)                                                                                                             aTTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAg                                                       >  8:74409/1‑131 (MQ=255)                                                                                                             aTTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAg                                                       <  7:74409/131‑1 (MQ=255)                                                                                                              ttCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGc                                                                                             >  5:200246/1‑92 (MQ=255)                                                                                                              ttCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGc                                                                                             <  6:200246/92‑1 (MQ=255)                                                                                                                 gcgcgACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCatgatg                                  <  7:117860/148‑1 (MQ=255)                                                                                                                   gcgACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCg                               <  2:188505/149‑1 (MQ=255)                                                                                                                        ggCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTc                          >  3:104018/1‑149 (MQ=255)                                                                                                                         gCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCGGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTcc                         <  5:295834/149‑1 (MQ=255)                                                                                                                           aTCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAg                       >  5:320662/1‑149 (MQ=255)                                                                                                                                 ccGGCAGACTCCTCGAACATG‑TCGAGGGAAA‑CCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGAcc                >  8:10200/1‑149 (MQ=255)                                                                                                                                    gCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGACCCa              <  6:104248/149‑1 (MQ=255)                                                                                                                                       tttccctcG‑ACATGTTCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGACCCAGc            >  7:82837/5‑148 (MQ=255)                                                                                                                                            ctcGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAg                                                       >  2:286009/1‑100 (MQ=255)                                                                                                                                            ctcGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAg                                                       <  1:286009/100‑1 (MQ=255)                                                                                                                                             tcGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGt                                                         <  2:334374/97‑1 (MQ=255)                                                                                                                                             tcGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGt                                                         >  1:334374/1‑97 (MQ=255)                                                                                                                                                aaCATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGACCCAGCCATCCCCGGa  <  4:170018/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                AGTGGCCTGGGCCAGTTGCAGCGGCGTGGCCTGCATATAGCCCTGGCCG‑ATGCCGAGAATCAGGGTTTCGCCAGGGAACCAGGCCTGGCGACGGGTGGCACGCTTCCATTCGCGCGACGGCATCAACCCGGCAGACTCCTCGAACATG‑TCGAGGGAAACCCGCTGGCCGATACCGAACTTGTTCATGTAGCTGGACAAGCGATCGATGCCCATCTTGTGCGCAAGGTCATAGAAGTAGGTGTCGTTCGAGCGCATGATGGCGGTGTCCAGGTCGACCCAGCCATCCCCGGA  >  NC_002947/5470507‑5470797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑