| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,126,645:1 | +C | 100% | intergenic (‑99/+50) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 82.4% | 90.8 / 16.5 | 34 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (6/0); new base C (8/20); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGC > NC_002947/1126498‑1126779 | tGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCCCGAATGCGGAGGTATATCCACCATCGCATTAGC‑GGG‑T‑AAA‑c > 1:195302/1‑149 (MQ=255) gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑cc < 8:21894/148‑1 (MQ=255) gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGATGGTGGCTATACCTCCGCATTCGC‑GGG‑T‑AAA‑cc < 2:197587/148‑1 (MQ=25) ccgatgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTcc > 6:313797/1‑148 (MQ=255) atCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCCCCATCGCAATCGC‑GGG‑TAAAA‑CCGCTCCTACACAGAGc > 8:255223/1‑149 (MQ=255) aCAGGTCGATGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCAc < 1:147923/149‑1 (MQ=255) cAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGCAATCGGAGGTATAGCCACCATCGCAATCGC‑GGG‑G‑AAACCCGCTCCTAACCCCAGCCCCCCAcc > 7:83215/1‑149 (MQ=255) gTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCaa < 8:3279/149‑1 (MQ=255) gTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCCCGAAAGAGGAGGAATAGCCACCAACGCATTCCCGGGG‑A‑AAA‑CCGCGACTACACACAACCCcccaccca > 6:78466/1‑148 (MQ=255) tCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCCCGCAAGGGGGGGTGTTGCCACCAACGCCATAGCGGGG‑T‑AAA‑CCCCGCCGACACAGCTCCCGCCCCCCaac > 3:100422/1‑148 (MQ=255) tCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCCCGAATGCGGAGGTATAGCCACCATAGCATTAGC‑GGG‑TAAAA‑CCGCTCCGACAAAGAGcccccccccccac > 2:97160/1‑146 (MQ=255) tCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAg < 5:313797/149‑1 (MQ=255) cTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGc < 7:197381/149‑1 (MQ=255) cAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCgg < 8:100974/149‑1 (MQ=255) aCCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg < 5:78466/149‑1 (MQ=255) gCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGccc < 2:22509/108‑1 (MQ=255) gCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGccc > 1:22509/1‑108 (MQ=255) gCCAGGGTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCt < 7:255223/149‑1 (MQ=255) cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGCGGAGGTATAGCCACCATCGCATTAGC‑GGGTT‑AAC‑CCGCGCCGACACAGAGCCCGCCCCCCAAGCCAGGTGGCGGGGAGGGCTATGAGGGGTCCTGa > 6:167502/1‑149 (MQ=255) ggTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCACATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAggg > 6:265642/1‑131 (MQ=255) ggTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCACATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAggg < 5:265642/131‑1 (MQ=255) ttgGGTGCACATTGAGCCCTGTCGGCGCGGGTTTACCCGCGAATGCGGAGTTATCGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGctct < 3:202359/149‑1 (MQ=38) aTTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACgg < 5:167502/149‑1 (MQ=255) ccTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgcg < 6:322139/149‑1 (MQ=255) ggCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTAGC‑GGGTT‑AAC‑CCGCTCCTACACAGAGCCCCCCACCCAAGCCAGGTGGCGGGGAGGGCTATAAGGCGGGCTGATAGCTCGCGATCGCCGGGCAGGCGCacac > 2:283394/1‑148 (MQ=38) gcgcGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCa < 4:100422/149‑1 (MQ=255) cgcgGGTTTACCCGCTAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGT‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAg < 3:153225/149‑1 (MQ=255) gcgGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAgg > 4:21919/1‑149 (MQ=255) ggTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTg < 1:97160/149‑1 (MQ=255) tttACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg < 2:195302/149‑1 (MQ=255) cccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTc < 1:293199/148‑1 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc < 8:83215/149‑1 (MQ=255) ccaccaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 3:165463/1‑149 (MQ=255) caccaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc > 1:116556/1‑149 (MQ=255) cATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc < 2:116556/149‑1 (MQ=255) cATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGATGTCGACCCGGcc < 6:115388/142‑1 (MQ=255) cATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGATGTCGACCCGGcc > 5:115388/1‑142 (MQ=255) | TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGC > NC_002947/1126498‑1126779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |