| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,656,016:1 | +C | 8.6% | coding (753/4527 nt) | PP_1449 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,656,016 | 1 | . | C | 8.6% | 214.6 / 16.4 | 58 | coding (753/4527 nt) | PP_1449 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (22/31); new base C (2/3); total (24/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01 | |||||||||||
CTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGCCTTTGCTGTCCACGCGCGGT > NC_002947/1655869‑1656163 | cTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATg‑g < 1:188568/149‑1 (MQ=255) gggCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGC‑CATGCGCGCCgg < 6:21404/148‑2 (MQ=255) ggCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAAc < 6:64822/149‑1 (MQ=255) cGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAg < 7:104878/149‑1 (MQ=255) tCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTTCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCt < 6:308406/149‑1 (MQ=255) gCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTgg < 5:301895/149‑1 (MQ=255) gCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTg < 1:104298/148‑1 (MQ=255) gCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTg < 3:66125/148‑1 (MQ=255) gCCACTGCAGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTg > 4:66125/1‑148 (MQ=255) ccACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCCCACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGATgg > 3:91604/1‑148 (MQ=255) ccACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGc < 5:244851/149‑1 (MQ=255) ccGCTGACATGCAGGCCCCCGGCCGATCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCa < 3:199203/147‑1 (MQ=255) gCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGC‑CATGCGCGCCgg < 7:243710/123‑2 (MQ=255) gCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGC‑CATGCGCGCCgg > 8:243710/1‑122 (MQ=255) aGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAggg > 7:298379/1‑149 (MQ=255) gCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGC‑CATGCGCGCCgg < 8:182051/119‑2 (MQ=255) gCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGC‑CATGCGCGCCgg > 7:182051/1‑118 (MQ=255) cGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGc < 2:108376/149‑1 (MQ=255) gCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAg < 5:146774/149‑1 (MQ=255) tGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCATACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGgt > 4:134141/1‑144 (MQ=255) tGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCATACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGgt < 3:134141/144‑1 (MQ=255) aTGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGTACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGac < 8:319723/149‑1 (MQ=255) aTGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGac > 1:78584/1‑149 (MQ=255) gACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTc > 8:289322/1‑118 (MQ=255) gACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTc < 7:289322/118‑1 (MQ=255) gACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCt > 7:48573/1‑149 (MQ=255) cgccTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATg‑g < 6:147613/88‑1 (MQ=255) ccccTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATg‑g > 5:147613/1‑90 (MQ=255) cccTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCtt < 2:131363/127‑2 (MQ=255) cccTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTg > 1:131363/1‑127 (MQ=255) gTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGGAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCg < 3:63839/149‑1 (MQ=255) ccAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTg > 3:282477/1‑149 (MQ=255) ccAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTg > 7:137541/1‑149 (MQ=255) cAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCgg > 8:1754/1‑145 (MQ=255) cAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCgg < 7:1754/145‑1 (MQ=255) gAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCg < 5:250209/149‑1 (MQ=255) aTCTGGGAGGCAACCTCTCGCAGACCGGCGGATTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCTCCGAACAGGGTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGa < 4:91604/148‑1 (MQ=255) cGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATa < 7:315005/145‑1 (MQ=255) cGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACgg < 2:78584/148‑1 (MQ=255) ggCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACAACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGTCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGc < 8:137541/149‑1 (MQ=255) gCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCg > 3:157172/1‑121 (MQ=255) gCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCg < 4:157172/121‑1 (MQ=255) cgcCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGc > 5:23092/1‑149 (MQ=255) gtagtaCTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCt < 8:48643/149‑1 (MQ=255) gtaCTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGc < 1:310455/138‑1 (MQ=255) gtaCTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGc > 2:310455/1‑138 (MQ=255) ttACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCAt > 5:336705/1‑148 (MQ=255) ccACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAg < 4:227113/148‑1 (MQ=255) cacaCGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCa < 6:324784/149‑1 (MQ=255) acacGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGc > 8:2912/1‑147 (MQ=255) cacGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGc > 5:226021/1‑146 (MQ=255) acGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGc > 4:332807/1‑149 (MQ=255) cGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCaa < 6:226021/146‑1 (MQ=255) aTACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGCCttt < 3:332807/149‑1 (MQ=255) aTACGCCCGGCGCGCATG‑GCGCCGAACACGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGCCttt < 4:282477/149‑1 (MQ=255) tACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGCCTTTg < 8:48573/149‑1 (MQ=255) gCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTAGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGCCTTTGCTg > 4:256703/1‑149 (MQ=255) ccGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGCCTTTGCTGTc < 3:145635/149‑1 (MQ=255) gcATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGCCTTTGCTGTCCAcgcgc > 4:317943/1‑149 (MQ=255) gcATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGCCTTTGCTGTCCAcgcgc > 4:127422/1‑149 (MQ=255) tG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGCCTTTGCTGTCCACGCGCGGt > 4:41622/1‑149 (MQ=255) | CTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTGCGAGGCCCCCACACGCACACCCGCGCCTTCGGTAGTACTTACCACACGGATACGCCCGGCGCGCATG‑GCGCCGAACAGGCTGGCATCAATGCTCGTGGAGGGGCTGGGCAGGTGTTCGACCACCTCGCCGCTGCGGCTGTCGATACGGTTGCGGCCGACAGTGATGTCGAGCGTTTCGCTGGCCATGAGCAAGCCTTTGCTGTCCACGCGCGGT > NC_002947/1655869‑1656163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |