| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 278,742:1 | +A | 100% | intergenic (‑237/+77) | PP_0224 ← / ← sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 278,742 | 1 | . | A | 90.0% | 119.7 / 6.2 | 40 | intergenic (‑237/+77) | PP_0224/sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base A (17/19); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGTCGCTACAGCTGATCGCGACAGACGTGTGCT‑GGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑A‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGC > NC_002947/278595‑278882 | aTGTCGCTACAGCTGATCGCGACAGACGTGTGCT‑GGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGt < 7:308059/148‑1 (MQ=255)aTGTCGCTACAGCTGATCGCGACAGACGTGTGCT‑GGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGt > 5:25805/1‑148 (MQ=255) gTCGCTACAGCTGATCGCGACAGACGTGTGCTGGGTCCGGTGGTCCGGTATATATACCGTAATGATTTATAAAATTTTTTTGAACTAACTTTTTGGATTATTTATTGAAGGGGGTTTTGTTACAGAGTTGCCGGGGTCTTCGCGGGT‑a > 1:220861/1‑148 (MQ=255) gTCGCTACAGCTGATCGCGACAGACGTGTGCT‑GGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑a‑aa > 4:309785/1‑148 (MQ=255) cAGCTGATCGCGACAGACGTGTGCT‑GGTCCGGTGGTCCGTTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGc < 5:318653/147‑1 (MQ=255) cTGATCGCGACAGACGTGTGCT‑GGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTa < 6:89629/149‑1 (MQ=255) aTCGCGACAGACGTGTGCT‑GGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTaca < 2:220861/148‑1 (MQ=255) cgACAGACGTGTGCT‑GGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACAt < 5:140026/149‑1 (MQ=255) aCGTGTGCT‑GGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTc > 5:146415/1‑148 (MQ=255) cT‑GGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTGCTACACACATGCCTCAAGATcgg > 6:317440/1‑147 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCtgtg > 1:285672/1‑148 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCtgt < 1:201982/147‑1 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCtgt < 2:273577/147‑1 (MQ=255) tccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTac < 3:118982/126‑1 (MQ=255) tccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTac > 4:118982/1‑126 (MQ=255) tccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑a‑aac < 6:124449/116‑3 (MQ=255) tccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑a‑aac > 5:124449/1‑114 (MQ=255) gtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCtgt < 6:350937/142‑1 (MQ=255) gtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCtgt > 5:350937/1‑142 (MQ=255) gtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTg > 6:3781/1‑148 (MQ=255) tggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa < 6:146415/149‑1 (MQ=255) gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAg < 3:178229/148‑1 (MQ=255) tccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAg > 8:39898/1‑147 (MQ=255) tccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCGGTGCACTGTAg > 8:34625/1‑147 (MQ=255) ccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTAGAGAGGTGCCGGGCTCTTCGCGGGT‑ATA‑CCCGCTCCTTCACACATGCCTCAAGATATGTGCACTGTAGGAg > 5:118217/1‑149 (MQ=255) gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCgg < 5:3781/149‑1 (MQ=255) atataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAg < 8:199900/139‑1 (MQ=255) atataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAg > 7:199900/1‑139 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCCCTACTAAACACATTCCTCAAGATCTGTGGACGGTAGGAGCGGGTTTACCcgc > 4:159384/1‑149 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTAc < 8:264197/143‑1 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGACGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTAc > 7:264197/1‑143 (MQ=255) taAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGt < 4:124931/119‑1 (MQ=255) taAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGt > 3:124931/1‑119 (MQ=255) ttattaACTAATTTTTTGTAATATTTATAGATGGGGTTTTTTTTACATCGGTGCCGGGCTCTTCGGGGGT‑A‑ACCCCGCTCCTACACACATCCCTCATGATCTGTGCACTGTCGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGtt < 2:285672/148‑1 (MQ=255) ggAATATTTATAGAAGGGGGATTTGTTACAGAGGGGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGt < 3:159384/148‑1 (MQ=255) gAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATGTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTcc > 5:2655/1‑149 (MQ=255) ggggcATTTGTAACAGAGGGGCCGGGCCCTGCCCGGGA‑A‑ACCCCGCTCCAACCCACAGGCCCCAAGATCTGGGCACGGTCGCAGGGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgcgc < 1:293611/149‑1 (MQ=255) gtaTTTGTTACAGAGGGGCCGGGCTCTTCGCGGGT‑A‑ACCCCGCTCCAACACACATGCCTCAAGATCTGTGCACTGTCGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAg < 7:34625/147‑1 (MQ=255) ggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGTTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAg < 7:39898/149‑1 (MQ=255) cacagGTGCCGGGAGCTTCGCGTGTAA‑A‑CCCGCTCCAACCAACATGCGTAAAGCTCTGTCCACTGTAGGCGCGTTTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCcgc < 6:25805/145‑1 (MQ=255) agagGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCcgcg > 7:38375/1‑148 (MQ=255) agGTGCCGGGCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGGACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTAc > 3:107433/1‑149 (MQ=255) gggCTCTTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGGCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGACCTTGCGCAAGAACTGCCGCGTACGCTcttc > 8:89775/1‑149 (MQ=255) tctTCGCGGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTcttccttc > 8:77943/1‑149 (MQ=255) cgcgGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGgc < 7:77943/148‑1 (MQ=255) cgcgGGTAA‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGgc < 6:2655/148‑1 (MQ=255) | ATGTCGCTACAGCTGATCGCGACAGACGTGTGCT‑GGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑A‑A‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGC > NC_002947/278595‑278882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |