Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	307,865:1	+C	91.3%	intergenic (+880/‑989)	hslO → / → PP_0254	molecular chaperone HslO/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	307,865	1	.	C	91.3%	218.8 / 14.7	69	intergenic (+880/‑989)	hslO/PP_0254	molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (3/3);  new base C (35/28);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

AAAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑G‑C‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTA  >  NC_002947/307717‑308006                                                                                                                                                         |                                                                                                                                               aaaGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑g                                                                                                                                                 <  6:233118/149‑1 (MQ=255)   aGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑G‑cc                                                                                                                                              <  8:8272/149‑2 (MQ=255)     ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTTTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑G‑ccgt                                                                                                                                            <  7:3710/149‑4 (MQ=255)     ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGTCAG‑C‑CGAAA‑G‑G‑Gta                                                                                                                                           <  2:300111/149‑2 (MQ=255)             tcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGAt                                                                                                                                    >  5:205512/1‑149 (MQ=255)                   cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTg                                                                                                                               <  7:196605/148‑1 (MQ=255)                           ccTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGAcgacga                                                                                                                        <  2:14440/147‑1 (MQ=255)                           ccTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAg                                                                                                                       <  6:147738/148‑1 (MQ=255)                             tgctgcCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGCAA‑GCC‑GTTACCTGATCGCTGACGACGACCAc                                                                                                                    >  6:246605/1‑149 (MQ=255)                                tgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGt                                                                                                                 >  6:196969/1‑149 (MQ=255)                                  ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGtt                                                                                                                <  5:196969/148‑1 (MQ=255)                                       tgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGc                                                                                                           >  4:26943/1‑148 (MQ=255)                                       cgccgatgttggccgcgcagtgcataggcagtacatcggcGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTga                                                                                                                              <  3:53694‑M2/89‑1 (MQ=255)                                       cgccgatgttggccgcgcagtgcataggcagtacatcggcGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTga                                                                                                                              >  4:53694‑M2/41‑129 (MQ=255)                                        gccgatgttggccgcgcagtgcataggcagtacatcggcGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCg                                                                                                          >  1:300662‑M2/40‑148 (MQ=255)                                          CGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑G‑CCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg                                                                                                       >  3:176410‑M1/1‑149 (MQ=255)                                               TACTGCCTATGGACTGCGCGGCCAACATCGGCTAAGAATGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑G‑CCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                  >  8:99723‑M1/1‑149 (MQ=255)                                                 cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTg                                                                                                 >  3:71553/1‑148 (MQ=255)                                                    ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑G‑CAGTTACATGATCGGTGAAGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt                                                                                             >  8:124748/1‑149 (MQ=255)                                                    ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACAATATGGGGTGCTGCAGTGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                               >  1:111468/1‑149 (MQ=255)                                                     cTATGCACTGCGCGGCCAACATCGGCGAAGTAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTT‑A‑CGTAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc                                                                                            <  5:246605/149‑1 (MQ=255)                                                     cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                  >  7:7241/1‑143 (MQ=255)                                                     cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                  <  8:7241/143‑1 (MQ=255)                                                      tATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc                                                                                            >  5:194002/1‑148 (MQ=255)                                                         gCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACa                                                                                        >  1:6010/1‑149 (MQ=255)                                                          cACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt                                                                                       <  4:71553/149‑1 (MQ=255)                                                           aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATg                                                                                      <  6:205512/149‑1 (MQ=255)                                                              ataggcagtacatcggcGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa                                                                                   <  2:300662‑M2/132‑1 (MQ=255)                                                                gcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAgg                                                                                 <  4:312737/149‑1 (MQ=255)                                                                   gCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcgg                                                                              >  5:97981/1‑149 (MQ=255)                                                                      aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                               <  5:122137/129‑1 (MQ=255)                                                                      aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                               >  6:122137/1‑129 (MQ=255)                                                                      aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgctg                                                                           >  3:322025/1‑149 (MQ=255)                                                                         atcggcGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTa                                                                        >  4:148440‑M2/7‑149 (MQ=255)                                                                            ggcGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                     >  4:263643‑M2/4‑149 (MQ=255)                                                                                     ggCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCAt                                                            <  3:263643/149‑1 (MQ=255)                                                                                        gATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGa                                                         >  5:265122/1‑149 (MQ=255)                                                                                         aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                        <  6:265122/149‑1 (MQ=255)                                                                                         aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                        <  6:194002/149‑1 (MQ=255)                                                                                         aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                        <  6:97981/149‑1 (MQ=255)                                                                                                   gTTCTTCGGCCTGTCCTGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCt                                                      <  8:27447/141‑1 (MQ=255)                                                                                                   gTTCTTCGGCCTGTCCTGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCt                                                      >  7:27447/1‑141 (MQ=255)                                                                                                    ttcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggc                                                                                >  8:213142/1‑114 (MQ=255)                                                                                                    ttcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggc                                                                                <  7:213142/114‑1 (MQ=255)                                                                                                    ttcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgag                                               >  3:93717/1‑147 (MQ=255)                                                                                                      cttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaag                                            >  5:258989/1‑148 (MQ=255)                                                                                                         cGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                         <  6:167482/148‑1 (MQ=255)                                                                                                           gCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt                                                                >  8:139435/1‑123 (MQ=255)                                                                                                           gCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt                                                                <  7:139435/123‑1 (MQ=255)                                                                                                              tGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                   >  7:162782/1‑149 (MQ=255)                                                                                                                tCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCgg                                  <  4:322025/148‑1 (MQ=255)                                                                                                                tCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGt                                 >  7:301370/1‑149 (MQ=255)                                                                                                                 ccGGCACTGGCCACACAACCCTTTCGGCTG‑A‑CGAAACC‑C‑GTTACCTCAGCGGTGACGGCGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCgggc                                <  4:255448/149‑3 (MQ=255)                                                                                                                  cGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGGTACGCCAAGTGCATCGACCTGTGCGAGAAGAACGAGCCGGTcg                               >  8:189122/1‑148 (MQ=255)                                                                                                                     cACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCt                            <  6:258989/149‑1 (MQ=255)                                                                                                                          gCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg                                                                                                       >  3:209419/1‑69 (MQ=255)                                                                                                                          gCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg                                                                                                       <  4:209419/69‑1 (MQ=255)                                                                                                                          gCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGaaa                       <  8:162782/149‑1 (MQ=255)                                                                                                                              gACTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAgg                                                                                 >  4:197746/1‑87 (MQ=255)                                                                                                                                cTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTTACGACGAGCACGGTTGGGGCTAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc                 <  7:189122/149‑1 (MQ=255)                                                                                                                                cTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                  >  3:227371/1‑148 (MQ=255)                                                                                                                                cTACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                  >  3:69162/1‑148 (MQ=255)                                                                                                                                 tACCCTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc                 >  7:282849/1‑148 (MQ=255)                                                                                                                                   cccTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa                                                                  >  6:81848/1‑97 (MQ=255)                                                                                                                                   cccTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg              >  7:280748/1‑149 (MQ=255)                                                                                                                                    ccTTTCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGc             <  8:301370/149‑1 (MQ=255)                                                                                                                                     cTTTCGGCTG‑A‑CGAAATC‑C‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCa            <  7:124748/149‑1 (MQ=255)                                                                                                                                        tCGGCTG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCAc           <  8:280748/147‑1 (MQ=255)                                                                                                                                             tG‑A‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg    >  5:60562/1‑149 (MQ=255)                                                                                                                                                a‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg              <  8:233546/137‑1 (MQ=255)                                                                                                                                                a‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg              >  7:233546/1‑137 (MQ=255)                                                                                                                                                a‑CGAAA‑GCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTa  >  6:310532/1‑149 (MQ=255)                                                                                                                                                         |                                                                                                                                               AAAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTG‑A‑CGAAA‑G‑C‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTA  >  NC_002947/307717‑308006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑