Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,620,299 C→A 7.7% A18A (GCG→GCT)  PP_3190 ← ornithine cyclodeaminase/mu‑crystallin family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,620,2990CA 7.7% 163.9 / 4.0 52A18A (GCG→GCT) PP_3190ornithine cyclodeaminase/mu‑crystallin family protein
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base A (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

TGAAGGTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCGGCGAGCCTCTG  >  NC_002947/3620160‑3620444
                                                                                                                                            |                                                                                                                                                  
tGAAGGTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTcc                                                                                                                                           <  6:67287/149‑1 (MQ=255)
  aaGGTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATTGGCGATGAAGCCTTCCTCCAGTTGGCGGTCAGCCAACtgga                                                                                                                                          <  6:178728/148‑4 (MQ=255)
  aaGGTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCGGTCAGCCAACtgga                                                                                                                                          <  5:69283/148‑4 (MQ=255)
     gTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGtt                                                                                                                                      >  2:117200/1‑149 (MQ=255)
              ccTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGc                                                                                                                             <  3:276449/149‑1 (MQ=255)
                ttCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGcga                                                                                                                           >  2:132369/1‑149 (MQ=255)
                ttCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGcga                                                                                                                           >  8:105028/1‑149 (MQ=255)
                  cGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTTCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGcga                                                                                                                           <  7:321198/147‑1 (MQ=255)
                     tGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATc                                                                                                                      >  3:291331/1‑149 (MQ=255)
                        aGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCg                                                                                                                                          <  8:215732/126‑1 (MQ=255)
                                   aCGCAACAGTCCCCGTTCGCGCCCTTGAATGCGACGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCCACTCCGGGTTGAAATGGGCGCAGATCTGCGCCTTGCTg                                                                                                          >  5:69859/1‑147 (MQ=255)
                                     gCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑Ga                                                                                                     >  2:216680/1‑149 (MQ=255)
                                       aaCAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GAcc                                                                                                   <  1:304735/149‑1 (MQ=255)
                                           gTCACCGTTCGCGCCCTTGAATGAGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCa                                                                                               <  6:69859/149‑1 (MQ=255)
                                            tCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCAt                                                                                              >  5:165631/1‑149 (MQ=255)
                                            tCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCAt                                                                                              <  2:328269/149‑1 (MQ=255)
                                             cACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCAtt                                                                                             >  5:174191/1‑149 (MQ=255)
                                             cACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCAt                                                                                              <  8:7073/148‑1 (MQ=255)
                                                    cgcgCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGc                                                                                      >  7:145106/1‑149 (MQ=255)
                                                     gcgcCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGcc                                                                                     >  8:262204/1‑149 (MQ=255)
                                                      cgcCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCt                                                                                    <  7:105028/149‑1 (MQ=255)
                                                         ccTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGCCCGCCCACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTc                                                                                   >  6:151988/1‑147 (MQ=255)
                                                         ccTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGa                                                                                 <  6:150500/149‑1 (MQ=255)
                                                          cTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAg                                                                                >  1:101943/1‑149 (MQ=255)
                                                           ttGAATGCGAAGCCCTGCACGGGCGGCACGCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGaa                                                                                                                                   >  1:26396/1‑99 (MQ=255)
                                                             gAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGaa                                                                             >  3:178775/1‑149 (MQ=255)
                                                                   gAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTc                                                                       >  8:149635/1‑149 (MQ=255)
                                                                     aGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGGACCCTCATTGAACGGCCTCGAGGAACTTTTcc                                                                      <  1:216680/149‑1 (MQ=255)
                                                                      gCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCcag                                                                    <  7:149635/149‑1 (MQ=255)
                                                                        ccTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCcagca                                                                  <  1:132369/149‑1 (MQ=255)
                                                                        ccTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCcagca                                                                  <  6:165631/149‑1 (MQ=255)
                                                                                 gcggcAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTAc                                                         <  6:174191/149‑1 (MQ=255)
                                                                                  cggcAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACg                                                        <  4:291331/149‑1 (MQ=255)
                                                                                  cggcAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACg                                                        <  2:108756/149‑1 (MQ=255)
                                                                                           gcacctTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTg                                                                                                             >  1:167311/1‑89 (MQ=255)
                                                                                           gcacctTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTg                                                                                                             <  2:167311/89‑1 (MQ=255)
                                                                                           gcacctTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCcaca                                                  >  1:173492/1‑147 (MQ=255)
                                                                                                     ttCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGc                                      >  5:143051/1‑149 (MQ=255)
                                                                                                            tAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGc                               <  2:101943/149‑1 (MQ=255)
                                                                                                             aGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCt                              >  4:70182/1‑149 (MQ=255)
                                                                                                                cGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGc                           <  1:117200/149‑1 (MQ=255)
                                                                                                                       gCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGa                      >  7:15969/1‑147 (MQ=255)
                                                                                                                             ccggaGTTGGCGGTCAGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCATGATAGCGTTc              >  4:80524/5‑149 (MQ=255)
                                                                                                                              tccAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGa                      <  6:276922/140‑1 (MQ=255)
                                                                                                                              tccAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGa                      >  5:276922/1‑140 (MQ=255)
                                                                                                                              tccAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGATCg             >  6:152981/1‑149 (MQ=255)
                                                                                                                              cggaGTTGGCGGTCAGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCTTTCg             >  4:15889/4‑149 (MQ=255)
                                                                                                                                cAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGaa                                                                                          <  4:35742/70‑1 (MQ=255)
                                                                                                                                cAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGaa                                                                                          >  3:35742/1‑70 (MQ=255)
                                                                                                                                cAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCg             <  6:143051/147‑1 (MQ=255)
                                                                                                                                       cTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCGGCGAGCctc    <  2:173492/149‑1 (MQ=255)
                                                                                                                                         gACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCGGCGAGCCTCTg  >  2:51698/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
TGAAGGTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCAC‑CTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAA‑GACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCGGCGAGCCTCTG  >  NC_002947/3620160‑3620444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑