Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,638,821 G→C 8.1% G273R (GGC→CGC)  PP_3205 → fumarylacetoacetate hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,638,8210GC 8.1% 231.7 / 8.1 73G273R (GGC→CGC) PP_3205fumarylacetoacetate hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (36/31);  new base C (3/3);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

GAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGCCATTGACCAGCGCGT  >  NC_002947/3638676‑3638969
                                                                                                                                                 |                                                                                                                                                    
gAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCt                                                                                                                                                   >  2:311544/1‑149 (MQ=255)
   cGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCgg                                                                                                                                                <  8:256294/149‑1 (MQ=255)
     gCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCCCACGCTGCGCTCGGCg                                                                                                                                              <  4:225421/149‑1 (MQ=255)
       gAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGTGCAGCGTGGGCTCGGCGTg                                                                                                                                            >  5:68482/1‑149 (MQ=255)
         gTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCa                                                                                                                                          >  1:6826/1‑149 (MQ=255)
            gTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCg                                                                                                                                       >  8:84407/1‑149 (MQ=255)
                   cATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAgcg                                                                                                                                >  6:161602/1‑149 (MQ=255)
                    aagtgcATGGCGCCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAgcgc                                                                                                                               >  3:321845/6‑149 (MQ=255)
                        ccATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAgcgccgcg                                                                                                                           >  4:130038/1‑149 (MQ=255)
                             gcggcgCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGTGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATc                                                                                                                      <  6:68482/149‑1 (MQ=255)
                                      tGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATc                                                                                                             <  3:130038/149‑1 (MQ=255)
                                        cACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCg                                                                                                            <  7:229677/148‑1 (MQ=255)
                                          cTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCTACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCACCGAGCGCCGCGCCATCGAAACCATCGCCt                                                                                                         <  1:30408/149‑1 (MQ=255)
                                           ttCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTg                                                                                                        >  6:20727/1‑149 (MQ=255)
                                           ttCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCAGCGAAACCATCGCCTg                                                                                                        >  6:339964/1‑149 (MQ=255)
                                             cGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTg                                                                                                        <  6:114431/147‑1 (MQ=255)
                                             cGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTg                                                                                                        <  7:84407/147‑1 (MQ=255)
                                                    caccaGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCgatgc                                                                                                                                          <  1:341904/106‑5 (MQ=255)
                                                    caccaGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCgatgc                                                                                                                                          >  2:341904/1‑102 (MQ=255)
                                                    caccaGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATc                                                                                                             >  1:22742/1‑135 (MQ=255)
                                                    caccaGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATc                                                                                                             <  2:22742/135‑1 (MQ=255)
                                                    caccaGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGcc                                                                                               >  6:12740/1‑149 (MQ=255)
                                                    caccaGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGcc                                                                                               >  7:128462/1‑149 (MQ=255)
                                                         gTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCcacgca                                                                                          >  2:138845/1‑149 (MQ=255)
                                                            gATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATTGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACgg                                                                                       >  6:249939/1‑149 (MQ=255)
                                                            gATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACgg                                                                                       >  4:138742/1‑149 (MQ=255)
                                                                 cccATGCGGCGCTGACGCGACGCTTGGGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTc                                                                                  <  6:128375/149‑1 (MQ=255)
                                                                 cccATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTc                                                                                  <  5:12740/149‑1 (MQ=255)
                                                                  ccATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCa                                                                                 >  8:53770/1‑149 (MQ=255)
                                                                       cggcgCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATgcgc                                                                            >  2:303968/1‑149 (MQ=255)
                                                                         gcgcTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCtt                                                                          <  1:303968/149‑1 (MQ=255)
                                                                           gcTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTg                                                                        <  5:20727/149‑1 (MQ=255)
                                                                            cTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCCCACGCTGCGCTCGGCGttg                                                                                                                                           <  4:248078/81‑3 (MQ=25)
                                                                            cTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCCCACGCTGCGCTCGGCGttg                                                                                                                                           >  3:248078/1‑79 (MQ=25)
                                                                               acgcgacgcTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGa                                                                    >  3:26784/1‑149 (MQ=255)
                                                                                 gcgacgcTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGTAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGAcc                                                                  >  4:267425/1‑149 (MQ=255)
                                                                                 gcgacgcTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGAcc                                                                  >  3:228975/1‑149 (MQ=255)
                                                                                     cgcTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGt                                                              <  8:128462/149‑1 (MQ=255)
                                                                                           aGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCAt                                                        <  6:87790/149‑1 (MQ=255)
                                                                                               cGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCTGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCgg                                                                >  5:50054/1‑137 (MQ=255)
                                                                                               cGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCTGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCgg                                                                <  6:50054/137‑1 (MQ=255)
                                                                                               cGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGa                                                     <  1:311544/148‑1 (MQ=255)
                                                                                               cGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGa                                                     <  3:138742/148‑1 (MQ=255)
                                                                                               cGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAg                                                    <  7:53770/149‑1 (MQ=255)
                                                                                                    aCGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTcg                                               >  5:256213/1‑149 (MQ=255)
                                                                                                      gCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCa                                                         <  4:38194/137‑1 (MQ=255)
                                                                                                      gCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCa                                                         >  3:38194/1‑137 (MQ=255)
                                                                                                       cTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCgg                                            <  5:161602/149‑1 (MQ=255)
                                                                                                              gCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAg                                     <  4:26784/149‑1 (MQ=255)
                                                                                                              gCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAg                                     <  4:321845/149‑1 (MQ=255)
                                                                                                                tCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAggg                                   >  8:165190/1‑149 (MQ=255)
                                                                                                                    gCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGTAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAg                               <  3:267425/149‑1 (MQ=255)
                                                                                                                       ccGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGGGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTg                            >  2:61672/1‑149 (MQ=255)
                                                                                                                         gtgtCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCt                          <  6:256213/149‑1 (MQ=255)
                                                                                                                           gtCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGt                        >  8:182320/1‑149 (MQ=255)
                                                                                                                            tCCAACGCCGAGCGCAGCGTGGGCTCGGCGTTCATCGCCGAGCGCCGCTCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCATTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGATGtt                       >  6:284060/1‑149 (MQ=255)
                                                                                                                            tCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATTGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGtt                       <  5:249939/149‑1 (MQ=255)
                                                                                                                            tCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGa                                <  2:233280/140‑1 (MQ=255)
                                                                                                                            tCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGa                                >  1:233280/1‑140 (MQ=255)
                                                                                                                              cAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAg                               >  8:298579/1‑139 (MQ=255)
                                                                                                                              cAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAg                               <  7:298579/139‑1 (MQ=255)
                                                                                                                                 cGCCGAGCCCACGCTGCGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTgggg                  <  5:125659/149‑1 (MQ=255)
                                                                                                                                 cGCCGAGCCCACGCTGCGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTgggg                  >  6:125659/1‑149 (MQ=255)
                                                                                                                                  gCCGAGCCCACGCTGCGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGc                 >  8:317803/1‑149 (MQ=255)
                                                                                                                                   ccGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGc                 >  2:36229/1‑148 (MQ=255)
                                                                                                                                    cGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGc                 >  8:131435/1‑147 (MQ=255)
                                                                                                                                    cGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGc                 <  7:131435/147‑1 (MQ=255)
                                                                                                                                     gAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCGTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGa                                      >  1:109718/1‑125 (MQ=255)
                                                                                                                                     gAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGa                                      <  2:109718/125‑1 (MQ=255)
                                                                                                                                     gAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGCCAt              >  8:228745/1‑149 (MQ=255)
                                                                                                                                      aGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAACCCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGCCAtt             <  4:40907/149‑1 (MQ=255)
                                                                                                                                         gcAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGCCATTGAc          >  4:168500/1‑149 (MQ=255)
                                                                                                                                               tGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGCCATTGACCAgcgc    >  5:314254/1‑149 (MQ=255)
                                                                                                                                                 ggCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGCCATTGACCAGcgcg   <  7:182320/148‑1 (MQ=255)
                                                                                                                                                 ggCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGCCATTGACCAGCGcgt  >  8:152815/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGAGCGCAGCGTGGGCTCGGCGTGCATCGCCGAGCGCCGCGCCATCGAAACCATCGCCTGCGGTGCGCCACGCACGGCCTTCATGCGCTTTGGCGACCGGGTGCGCATGGAGGCTCGCGGGCCCGAGGGCGAGGTGCTGTTTGGGGCCATTGACCAGCGCGT  >  NC_002947/3638676‑3638969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑