Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,641,822 T→C 7.4% A461A (GCA→GCG)  PP_3209 ← isoxanthopterin deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,641,8220TC 7.4% 154.2 / 2.7 54A461A (GCA→GCG) PP_3209isoxanthopterin deaminase
Reads supporting (aligned to +/- strand):  ref base T (24/26);  new base C (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

GAAGGGCAGGGGAGCAGGCCCCGGCGGGGCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCG  >  NC_002947/3641679‑3641968
                                                                                                                                               |                                                                                                                                                  
gAAGGGCAGGGGAGCAGGCCCCGGCGGGGCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTg                                                                                                                                               >  3:234556/1‑149 (MQ=255)
 aaGGGCAGGGGAGCAGGCCCCGGCGGGGCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTgg                                                                                                                                              <  5:202081/149‑1 (MQ=255)
  aGGGCAGGGGAGCAGGCCCCGGCGGGGCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGc                                                                                                                                             <  6:312587/149‑1 (MQ=255)
   gggCAGGGGAGCAGGCCCCGGCGGGGCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCAGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCAGGCACGCCAGGCt                                                                                                                                            <  8:223621/149‑1 (MQ=255)
             gCAGGCCCCGGCGGGGCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCg                                                                                                                                  >  8:98946/1‑149 (MQ=255)
             gCAGGCCCCGGCGGGGCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCg                                                                                                                                  <  3:148500/149‑1 (MQ=255)
              cAGGCCCCGGCGGGGCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCgg                                                                                                                                 <  7:295177/149‑1 (MQ=255)
                 gCCCCGGCGGGGCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGc                                                                                                                                >  3:191186/1‑147 (MQ=255)
                         ggggCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAg                                                                                                                      <  8:185028/149‑1 (MQ=255)
                          gggCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGATGTACAGCCAGGCACGCCAGGCTgta                                                                                                                                         <  1:65971/129‑3 (MQ=255)
                          gggCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGATGTACAGCCAGGCACGCCAGGCTgta                                                                                                                                         >  2:65971/1‑127 (MQ=255)
                            gCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCa                                                                                                                   <  5:154216/149‑1 (MQ=255)
                                   ccACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATc                                                                                                             <  3:149681/148‑1 (MQ=255)
                                    cacaCCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCg                                                                                                            >  7:57604/1‑148 (MQ=255)
                                         ccAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGt                                                                                                      >  5:179173/1‑149 (MQ=255)
                                          cAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGtt                                                                                                     >  3:283094/1‑149 (MQ=255)
                                           aGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGttt                                                                                                    >  1:45833/1‑149 (MQ=255)
                                            gCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGtttt                                                                                                   <  2:45833/149‑1 (MQ=255)
                                              aCGTCGTCTGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTcc                                                                                                 <  3:293995/149‑1 (MQ=255)
                                              aCGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTcc                                                                                                 <  8:315170/149‑1 (MQ=255)
                                                gtcgtcGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCAc                                                                                               <  4:234556/149‑1 (MQ=255)
                                                         ttCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAggcg                                                                                      >  2:180877/1‑149 (MQ=255)
                                                                  cAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAg                                                                                         <  6:70227/137‑1 (MQ=255)
                                                                  cAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAg                                                                                         >  5:70227/1‑137 (MQ=255)
                                                                      gCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAg                                                                          >  2:264113/1‑148 (MQ=255)
                                                                       cccGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGc                                                                         >  8:156884/1‑148 (MQ=255)
                                                                          gCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGCACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAg                                                                                                                             <  4:279271/93‑1 (MQ=255)
                                                                          gCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGCACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAg                                                                                                                             >  3:279271/1‑93 (MQ=255)
                                                                            aCCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTACAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGcc                                                                   >  3:160079/1‑149 (MQ=255)
                                                                              cTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTcc                                                                                                 <  4:255760/117‑1 (MQ=255)
                                                                              cTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTcc                                                                                                 >  3:255760/1‑117 (MQ=255)
                                                                              cTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCg                                                                 >  6:300287/1‑149 (MQ=255)
                                                                                gCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCg                                                                 >  3:115575/1‑147 (MQ=255)
                                                                                  agagGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGc                                                             <  7:98946/149‑1 (MQ=255)
                                                                                  agagGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGc                                                             >  4:285585/1‑149 (MQ=255)
                                                                                       cGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCgg                                                        <  8:314728/149‑1 (MQ=255)
                                                                                                     gcgTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCaa                                          <  7:209680/149‑1 (MQ=255)
                                                                                                       gTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAAc                                         >  5:225773/1‑148 (MQ=255)
                                                                                                              cgcgGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTgggg                                 <  5:300287/149‑1 (MQ=255)
                                                                                                                     tGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCg                               >  3:284750/1‑144 (MQ=255)
                                                                                                                     tGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCg                               <  4:284750/144‑1 (MQ=255)
                                                                                                                           aGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCa                        >  5:149788/1‑145 (MQ=255)
                                                                                                                           aGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCa                        <  6:149788/145‑1 (MQ=255)
                                                                                                                            ggCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCCCGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCa                   >  6:79855/1‑149 (MQ=255)
                                                                                                                            ggCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCa                   <  4:115575/149‑1 (MQ=255)
                                                                                                                            ggCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCa                   <  3:285585/149‑1 (MQ=255)
                                                                                                                             gCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCAt                  <  4:283094/149‑1 (MQ=255)
                                                                                                                                  gggcAGCCAGGCACGCCAGGCTGCCCAGTTCGGCGAGGTCCAAGACAGGAATCGCCACGTGTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGACGTACGGACACCGCCAACCAATTGGGCCATGCCCAGATCAAGCAGa             >  7:285398/4‑149 (MQ=255)
                                                                                                                                   tACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGAc            <  6:225773/149‑1 (MQ=255)
                                                                                                                                   tACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGAc            >  4:147590/1‑149 (MQ=255)
                                                                                                                                        ccTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAg       >  8:126977/1‑149 (MQ=255)
                                                                                                                                          tGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAg       >  5:318749/1‑147 (MQ=255)
                                                                                                                                             cGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCg  <  3:147590/149‑1 (MQ=255)
                                                                                                                                             cGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCg  <  6:179173/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GAAGGGCAGGGGAGCAGGCCCCGGCGGGGCCAGTTCCACACCCAGCACGTCGTCGGCTTCAAGGCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCG  >  NC_002947/3641679‑3641968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑