Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,718,088 C→G 11.2% E169Q (GAG→CAG)  paaG ← 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,718,0880CG11.2% 127.9 / 5.5 44E169Q (GAG→CAG) paaG2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base C (21/18);  new base G (2/3);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGC  >  NC_002947/3717940‑3718232
                                                                                                                                                     |                                                                                                                                                 
ttGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTc                                                                                                                                                   <  7:36226/149‑1 (MQ=255)
   aGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTGGc                                                                                                                                                 >  4:322134/1‑148 (MQ=255)
      cTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGTGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAg                                                                                                                                             <  4:105218/149‑1 (MQ=255)
                   gtgtCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTGGCCATGCTGGGCGAGCGcc                                                                                                                                <  3:322134/149‑1 (MQ=255)
                   gtgtCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGcc                                                                                                                                >  4:206661/1‑149 (MQ=255)
                    tgtCAGGCCGTAGGTGGGCTGGGCGGCGAGGGGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTGGCCATGCTGGGCGAGCGCCt                                                                                                                               <  5:341647/149‑1 (MQ=255)
                                 gTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCAt                                                                                                                  >  2:26209/1‑149 (MQ=255)
                                   gggctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGc                                                                                                                >  2:185558/1‑149 (MQ=255)
                                                    ggcgggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGgcag                                                                                                >  8:97531/1‑148 (MQ=255)
                                                          cgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCaa                                                                                         <  6:147931/149‑1 (MQ=255)
                                                          cgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCaa                                                                                         <  8:179202/149‑1 (MQ=255)
                                                              ggTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTAGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTAc                                                                                     >  6:71633/1‑149 (MQ=255)
                                                                    ggCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTAccgccg                                                                                >  6:147732/1‑148 (MQ=255)
                                                                      cTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAg                                                                             >  4:235213/1‑149 (MQ=255)
                                                                             aCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCa                                                                      <  3:206661/149‑1 (MQ=255)
                                                                             aCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCa                                                                      >  2:258166/1‑149 (MQ=255)
                                                                              cGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAc                                                                     <  3:235213/149‑1 (MQ=255)
                                                                                      tGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGGCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTAccgccg                                                                                <  1:110442/130‑1 (MQ=255)
                                                                                      tGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTAccgccg                                                                                >  2:110442/1‑130 (MQ=255)
                                                                                        catcatCCACCACGCGGTAGATCAGCTCCCATTGCTCGGCCTGTTCGGACGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGa                                                             >  1:255119/1‑148 (MQ=255)
                                                                                          tcatcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCtt                                                         <  1:26209/149‑1 (MQ=255)
                                                                                             tcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                        >  4:66692/1‑147 (MQ=255)
                                                                                                       ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                            <  5:147732/149‑1 (MQ=255)
                                                                                                       ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTg                                             >  2:46043/1‑148 (MQ=255)
                                                                                                       ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTg                                             >  5:195323/1‑148 (MQ=255)
                                                                                                                  ccccATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGc                                <  7:97531/149‑1 (MQ=255)
                                                                                                                   cccATTGCTCGGCCTGTTCGG‑CGCCAACGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGcc                               >  4:51296/1‑149 (MQ=255)
                                                                                                                      aTTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAg                            >  3:314536/1‑149 (MQ=255)
                                                                                                                             ggCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAgcgggcgg                     <  3:51296/149‑1 (MQ=255)
                                                                                                                              gCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGggcggc                    <  1:185558/149‑1 (MQ=255)
                                                                                                                               ccTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGcc                   <  4:314536/149‑1 (MQ=255)
                                                                                                                               ccTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGcc                   >  5:258124/1‑149 (MQ=255)
                                                                                                                                cTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCa                  <  6:195323/149‑1 (MQ=255)
                                                                                                                                 tGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAg                 >  8:299753/1‑149 (MQ=255)
                                                                                                                                      gg‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCa                  <  5:180814/143‑1 (MQ=255)
                                                                                                                                      gg‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCa                  >  6:180814/1‑143 (MQ=255)
                                                                                                                                      gg‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCa            <  3:317856/149‑1 (MQ=255)
                                                                                                                                           ccAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGCATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGt         <  1:46043/149‑1 (MQ=255)
                                                                                                                                            cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCa                  <  4:47375/138‑1 (MQ=255)
                                                                                                                                            cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCa                  >  3:47375/1‑138 (MQ=255)
                                                                                                                                            cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg       >  2:116538/1‑149 (MQ=255)
                                                                                                                                              aGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGc      <  5:5961/148‑1 (MQ=255)
                                                                                                                                              aGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGc      >  6:5961/1‑148 (MQ=255)
                                                                                                                                              aGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCa     <  2:255119/149‑1 (MQ=255)
                                                                                                                                                  gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGc  >  8:98745/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGG‑CGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGC  >  NC_002947/3717940‑3718232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑