| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,727,128 | T→A | 10.0% | L114Q (CTG→CAG) | PP_3292 → | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,727,128 | 0 | T | A | 10.0% | 158.4 / 8.4 | 60 | L114Q (CTG→CAG) | PP_3292 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (25/29); new base A (2/4); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01 | |||||||||||
GCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGG > NC_002947/3726983‑3727271 | gCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTggg < 6:13611/149‑1 (MQ=255)gCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTggg < 2:289529/149‑1 (MQ=255) ggTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGca < 1:243834/149‑1 (MQ=255) ggTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGca < 5:121115/149‑1 (MQ=255) ggTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGca < 1:122310/149‑1 (MQ=255) cGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAc < 4:148988/149‑1 (MQ=255) cGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAc < 8:253741/149‑1 (MQ=255) gaggCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCAcc < 1:50673/147‑1 (MQ=255) gCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCttt < 6:249791/149‑1 (MQ=255) gAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGc < 7:285123/139‑1 (MQ=255) gAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGc > 8:285123/1‑139 (MQ=255) cgctgcACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtggct < 5:125266/145‑1 (MQ=255) cAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGAGTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGcac > 7:209830/1‑120 (MQ=255) cAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGcac < 8:209830/120‑1 (MQ=255) aaaGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGt > 5:58047/1‑149 (MQ=255) aaaGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGt > 3:30089/1‑149 (MQ=255) gCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCGCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCCCCTTTTGGCTGGCAGGGCGCTCAGTGAc > 5:160149/1‑149 (MQ=255) aTTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACg > 6:310760/1‑147 (MQ=255) aaCGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGt > 8:258250/1‑149 (MQ=255) agaCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGc < 5:310760/149‑1 (MQ=255) aCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGc > 1:67064/1‑147 (MQ=255) aCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGa < 7:258250/149‑1 (MQ=255) cAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAg < 8:209923/149‑1 (MQ=255) aGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCtt > 3:328821/1‑102 (MQ=255) aGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCtt < 4:328821/102‑1 (MQ=255) gCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGcc < 2:67064/149‑1 (MQ=255) tGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCaa > 1:84193/1‑149 (MQ=255) ggCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCa < 6:268728/116‑1 (MQ=255) ggCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCa > 5:268728/1‑116 (MQ=255) gCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAAcc > 7:268452/1‑149 (MQ=255) gCAGGCCAGACTGGCGTTCATTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGATGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAAATGCGAGGCCAAcc > 2:259986/1‑149 (MQ=255) cAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCt < 2:84193/149‑1 (MQ=255) ggCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGc < 6:58047/149‑1 (MQ=255) gCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGc < 4:180134/82‑1 (MQ=255) gCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGc > 3:180134/1‑82 (MQ=255) gCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCa < 8:268452/149‑1 (MQ=255) gCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTc > 7:285849/1‑145 (MQ=255) gCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTc < 8:285849/145‑1 (MQ=255) gTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTgg < 4:30089/149‑1 (MQ=255) ttCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGca < 6:197667/70‑1 (MQ=255) ttCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGca > 5:197667/1‑70 (MQ=255) cGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGAtata < 6:263303/147‑1 (MQ=255) gTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCg > 4:33428/1‑145 (MQ=255) gCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATg > 4:127895/1‑148 (MQ=255) gCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATg > 5:105939/1‑148 (MQ=255) gggAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGaaaaa > 7:250300/1‑149 (MQ=255) gggAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGaaa < 3:33428/147‑1 (MQ=255) aTCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCa < 8:250300/149‑1 (MQ=255) aaCATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACcgc > 1:282383/1‑149 (MQ=255) ctgGTGGTGCTGTTGTGCCTG‑GCCCTGCTTGGCACAGGAACCTTTTGGCTGGCAGGGCGCTCAGTGAAGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGTCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTtctc < 1:259986/147‑1 (MQ=255) tggtggTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGaa < 7:20851/122‑1 (MQ=255) tggtggTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGaa > 8:20851/1‑122 (MQ=255) gCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCgg > 3:175287/1‑149 (MQ=255) tgttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGgtg < 6:105939/149‑1 (MQ=255) gttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAgg > 4:76604/1‑80 (MQ=255) gttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAgg < 3:76604/80‑1 (MQ=255) gtgCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGc < 6:160149/148‑1 (MQ=255) cccTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTg > 3:128601/1‑149 (MQ=255) ccTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTgg > 2:185424/1‑149 (MQ=255) ccTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTgg > 2:322607/1‑149 (MQ=255) | GCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGG > NC_002947/3726983‑3727271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |