Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,747,271 T→A 6.1% K497M (AAG→ATG)  kefB‑II ← glutathione‑regulated potassium/H+ antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,747,2710TA 6.1% 195.8 / 2.8 65K497M (AAG→ATG) kefB‑IIglutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base T (28/33);  new base A (2/2);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

AGCTTCAACGCTCGAGGCTCCCAAGGTCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGGCAGCATGCAACAGGTCATGCCGGGCACCATCACCGTAATAGACCTTGA  >  NC_002947/3747126‑3747418
                                                                                                                                                 |                                                                                                                                                   
aGCTTCAACGCTCGAGGCTCCCAAGGTCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCa                                                                                                                                                  >  7:296974/1‑149 (MQ=255)
      aaCGCTCGAGGCTCCCAAGGTCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGc                                                                                                                                             <  2:142591/148‑1 (MQ=255)
         gCTCGAGGCTCCCAAGGTCAGCACGGCCTGGCGTCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGc                                                                                                                                         <  7:186276/149‑1 (MQ=255)
             gAGGCTCCCAAGGTCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTTAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAAt                                                                                                                                      >  8:80666/1‑148 (MQ=255)
             gAGGCTCCCAAGGTCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATa                                                                                                                                     >  5:328618/1‑149 (MQ=255)
               ggCTCCCAAGGTCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACt                                                                                                                                   <  2:129803/149‑1 (MQ=255)
                      aaGGTCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCATCACCTGCGGGCCATCCTGCCGCGt                                                                                                                            >  8:30045/1‑149 (MQ=255)
                      aaGGTCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCATCACCTGCGGGCAATACTGCCGCGt                                                                                                                            >  8:24991/1‑149 (MQ=255)
                          tCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGc                                                                                                                                 <  6:201605/140‑1 (MQ=255)
                          tCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGc                                                                                                                                 >  5:201605/1‑140 (MQ=255)
                            aGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTc                                                                                                                      >  6:70830/1‑149 (MQ=255)
                             gCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCaa                                                                                                                                       <  3:13324/131‑1 (MQ=255)
                             gCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCaa                                                                                                                                       >  4:13324/1‑131 (MQ=255)
                             gCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTc                                                                                                                      >  8:118159/1‑148 (MQ=255)
                              cACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTcga                                                                                                                    <  8:296974/149‑1 (MQ=255)
                               aCGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTcgac                                                                                                                   <  6:284664/149‑1 (MQ=255)
                                 ggCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGCTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATAATGACGCGTGCTGTcgacga                                                                                                                 >  4:229347/1‑149 (MQ=255)
                                         ggcCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTc                                                                                                         <  8:263990/149‑1 (MQ=255)
                                              agcagcAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCCGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGc                                                                                                    >  1:114678/1‑149 (MQ=255)
                                              agcagcAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCATCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGc                                                                                                    <  7:24991/149‑1 (MQ=255)
                                              agcagcAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGGCAAAGGCGCGTACCAGCACCATCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGc                                                                                                    <  7:30045/149‑1 (MQ=255)
                                               gcagcaAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGc                                                                                                                                             >  3:258886/1‑107 (MQ=255)
                                               gcagcaAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGc                                                                                                                                             <  4:258886/107‑1 (MQ=255)
                                               gcagcaAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGcc                                                                                                   <  7:118159/149‑1 (MQ=255)
                                                 agcaAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCctt                                                                                                 <  2:114678/149‑1 (MQ=255)
                                                  gcaAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCcttc                                                                                                <  6:162157/149‑1 (MQ=255)
                                                        atgaTTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGCTCCATCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTc                                                                                           <  3:229347/146‑1 (MQ=255)
                                                        aggaTTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTTACCAGTTCCAGAGCGTGCTCGCGGTCAAAGGCGCTTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCa                                                                                          <  7:24746/149‑1 (MQ=255)
                                                           aTTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTc                                                                                       <  8:247215/149‑1 (MQ=255)
                                                            ttCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCa                                                                                      <  5:70830/149‑1 (MQ=255)
                                                               aaaGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACg                                                                                    >  7:250772/1‑148 (MQ=255)
                                                               aaaGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACg                                                                                    >  7:174938/1‑148 (MQ=255)
                                                               aaaGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACg                                                                                    >  6:136950/1‑148 (MQ=255)
                                                               aaaGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTGCTTGTCATTCACg                                                                                    >  3:21352/1‑148 (MQ=255)
                                                                aaGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGca                                                                                  <  8:250772/149‑1 (MQ=255)
                                                                aaGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGca                                                                                  <  5:259691/149‑1 (MQ=255)
                                                                aaGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGca                                                                                  >  1:260124/1‑149 (MQ=255)
                                                                   gTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGcacac                                                                               <  6:328618/149‑1 (MQ=255)
                                                                    tttCCCGCACGATGTAGTCGGCATCGTGATTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCTTGTCGCGGCCTTCTTGTCATTCACGcacac                                                                               >  8:324400/1‑148 (MQ=255)
                                                                         cGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAatgat                                                                         >  8:150656/1‑149 (MQ=255)
                                                                         cGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAatgag                                                                         >  1:294761/1‑148 (MQ=255)
                                                                          gctcGATGTAGTCGGGATCGTGCTTGACCAGTTCCTGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGTGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAatgatg                                                                        <  4:21352/146‑1 (MQ=255)
                                                                          gCACGATGTAGTCGGCATCGGGCTTGACGAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCCCCTGCGGGCAATACTGCCGCGGGGTGTCGAGGGTGCGTGTCGCGGCGTTCTTGACCTTCCCGCACAGCatgatg                                                                        >  8:159362/1‑149 (MQ=255)
                                                                                tGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCgg                                                                  <  5:334115/149‑1 (MQ=255)
                                                                                tGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCgg                                                                  >  6:334115/1‑149 (MQ=255)
                                                                                        gCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACg                                                          <  5:136950/149‑1 (MQ=255)
                                                                                              tGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGcccc                                                    >  5:77610/1‑149 (MQ=255)
                                                                                               gCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGTTGAGGGCGTGACGGGCCCCg                                                   >  8:146172/1‑149 (MQ=255)
                                                                                                cTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCgg                                                  >  5:116960/1‑149 (MQ=255)
                                                                                                cTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCgg                                                  <  7:80666/149‑1 (MQ=255)
                                                                                                    aCCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGgcagc                                              <  6:116960/149‑1 (MQ=255)
                                                                                                    aCCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGgcag                                               <  7:324400/148‑1 (MQ=255)
                                                                                                       aGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGGCAGCATg                                           <  7:150656/149‑1 (MQ=255)
                                                                                                                gcgTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGGCAGCATGCAACAGGTc                                  <  5:109000/149‑1 (MQ=255)
                                                                                                                      tCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTcgacga                                                                                                                 <  7:28995/64‑1 (MQ=255)
                                                                                                                      tCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTcgacga                                                                                                                 >  8:28995/1‑64 (MQ=255)
                                                                                                                      tCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGGCAGCATGCAACAGGTCATGCCg                            >  6:289286/1‑149 (MQ=255)
                                                                                                                                  gcgcGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGGCAGCATGCAACAGGTCATGCCGGGCACCATCAcc                <  6:77610/149‑1 (MQ=255)
                                                                                                                                      gTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGgca                                                <  6:212076/113‑1 (MQ=255)
                                                                                                                                      gTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGgca                                                >  5:212076/1‑113 (MQ=255)
                                                                                                                                        aCCAGCACCTTCACCTGCGGGCATTACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGGCTTCTTGTCATTCACGCACACAATGATGGTGCGTGCGTGACGGGCCCCGGCAGCATGCAACAGGTCATGCCGGGCACCATCACCGTAAt           <  3:113033/148‑1 (MQ=255)
                                                                                                                                          cAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGGCAGCATGCAACAGGTCATGCCGGGCACCATCACCGTAATAGa        >  8:54189/1‑149 (MQ=255)
                                                                                                                                            gCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGGCAGCATGCAACAGGTCATGCCGGGCACCATCACCGTAATAGAcc      <  5:289286/149‑1 (MQ=255)
                                                                                                                                                cTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGgcagc                                              <  2:232534/105‑1 (MQ=255)
                                                                                                                                                cTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGgcagc                                              >  1:232534/1‑105 (MQ=255)
                                                                                                                                                 ttCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGGCAGCATGCAACAGGTCATGCCGGGCACCATCACCGTAATAGACCTTGa  >  3:106799/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGCTTCAACGCTCGAGGCTCCCAAGGTCAGCACGGCCTGGCGGCCAAGCAGCAAGGAGGATTCAAAGGTTTCCCGCACGATGTAGTCGGCATCGTGCTTGACCAGTTCCAGCGCGTGCTCGCGGTCAAAGGCGCGTACCAGCACCTTCACCTGCGGGCAATACTGCCGCGTGCTTTCGACGATGCGTGTCGCGGCCTTCTTGTCATTCACGCACACAATGATGGTGCGGGCGTGACGGGCCCCGGCAGCATGCAACAGGTCATGCCGGGCACCATCACCGTAATAGACCTTGA  >  NC_002947/3747126‑3747418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑