Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,762,942 A→T 9.4% N62I (AAC→ATC)  PP_3326 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,762,9420AT 9.4% 138.9 / 3.5 52N62I (AAC→ATC) PP_3326hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (19/27);  new base T (2/3);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCG  >  NC_002947/3762795‑3763080
                                                                                                                                                     |                                                                                                                                          
tGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGaa                                                                                                                                            >  3:126767/1‑148 (MQ=255)
    ggTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATg                                                                                                                                        >  1:251244/1‑149 (MQ=255)
      tCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATc                                                                                                                                     <  5:199009/149‑1 (MQ=255)
      tCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATc                                                                                                                                     <  8:166479/149‑1 (MQ=255)
      tCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATc                                                                                                                                     <  6:126100/149‑1 (MQ=255)
      tCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATc                                                                                                                                     >  6:332851/1‑149 (MQ=255)
          cGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCgg                                                                                                                                 <  7:3884/149‑1 (MQ=255)
              aaCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCC‑AGGCGCTCGACCGTGACCGGGATCATGTTCCCGGTCa                                                                                                                              <  1:241160/148‑1 (MQ=38)
                    ggCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGa                                                                                                                       <  2:114507/149‑1 (MQ=255)
                       gcCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTCCCGGTCACgtcgaacg                                                                                                                     >  4:75965/1‑142 (MQ=38)
                        cccACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTCCCGGTCAC‑GTCGAacgc                                                                                                                   <  2:251244/149‑1 (MQ=38)
                            cGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTCCCGGTCAC‑GTCGAACGcccag                                                                                                               <  3:75965/149‑4 (MQ=37)
                             ggCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGc                                                                                                              <  2:203370/149‑1 (MQ=255)
                                cTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGa                                                                                                           <  4:126767/149‑1 (MQ=255)
                                   tgGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGAt                                                                                                          <  5:280056/147‑1 (MQ=255)
                                     gACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATg                                                                                                      >  7:34612/1‑149 (MQ=255)
                                     gACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATg                                                                                                      <  5:332851/149‑1 (MQ=255)
                                          aggagCGACGTGGCAATATCGAGGCCGTGGACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtg                                                                                                 <  3:280017/149‑1 (MQ=255)
                                           ggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtg                                                                                                 <  1:295843/148‑1 (MQ=255)
                                            gatcgtcGTTGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGTGAACATGATCCCGGTCACGGTCGAGCGCGTGGCGGATCATGCCGtgct                                                                                               <  5:192089/143‑1 (MQ=255)
                                            gagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgct                                                                                               >  2:253640/1‑149 (MQ=255)
                                            gagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgct                                                                                               <  6:48784/149‑1 (MQ=255)
                                             agCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgctg                                                                                              <  1:253640/149‑1 (MQ=255)
                                             agCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtg                                                                                                 >  3:81548/1‑146 (MQ=255)
                                             agCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtg                                                                                                 <  4:81548/146‑1 (MQ=255)
                                                  cGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAAcc                                                                                         <  1:132275/149‑1 (MQ=255)
                                                       cAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAGCATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCACCCACTTa                                                                                    >  1:153195/1‑149 (MQ=255)
                                                            tCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCg                                                                               >  6:2324/1‑149 (MQ=255)
                                                             cgtggCCGTACACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGc                                                                              >  8:136470/4‑149 (MQ=255)
                                                                    gtgtACGGCCACGGCGCCGAGGATAACGCCTACAGAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGATCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCgg                                                                        >  1:47095/1‑149 (MQ=255)
                                                                        aCGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACtt                                                                   >  4:236357/1‑149 (MQ=255)
                                                                         cGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTg                                                                  >  2:48356/1‑149 (MQ=255)
                                                                                 gcgcCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgctg                                                          <  7:136470/149‑1 (MQ=255)
                                                                                    ccGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGctgctg                                                       <  1:229105/149‑1 (MQ=255)
                                                                                    ccGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGctgctg                                                       >  8:103797/1‑149 (MQ=255)
                                                                                     cGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGctgctg                                                       >  2:199421/1‑148 (MQ=255)
                                                                                     cGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTgg                                                      <  5:2324/149‑1 (MQ=255)
                                                                                      gAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgctgc                                                         <  8:157070/145‑1 (MQ=255)
                                                                                      gAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgctgc                                                         >  7:157070/1‑145 (MQ=255)
                                                                                      gAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGa                                                     <  7:100931/149‑1 (MQ=255)
                                                                                       aGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGAc                                                    <  1:48356/149‑1 (MQ=255)
                                                                                         gATAACGCCTACA‑AGGCGGAAAAGATCAAGCTCGCCTGGGCGTTCGA‑CGTGACCGGGAACCTGATACCGGTCACGGTCGAGCGCCTTGCGGATCATGCCGTGCTGGAACCACTTACCCCGACTGCGGCACTTGCCGTGCTGCTGGACaa                                                  >  7:231958/1‑149 (MQ=255)
                                                                                           tAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACg                                                <  3:236357/149‑1 (MQ=255)
                                                                                                    cA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGt                                       <  2:243540/149‑1 (MQ=255)
                                                                                                    cA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGATCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                         <  2:47095/147‑1 (MQ=255)
                                                                                                           ggAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGCGAACAAGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACACCGCCTGCGTCACTTGCCCTGATGCTGGACAACGGGCCTTGGTCACAgc                                 >  3:85172/1‑149 (MQ=255)
                                                                                                            gAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATc                                                                                                         >  7:175137/1‑76 (MQ=255)
                                                                                                            gAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATc                                                                                                         <  8:175137/76‑1 (MQ=255)
                                                                                                             aaaaGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAgcgc                               >  6:137111/1‑149 (MQ=255)
                                                                                                             aaaaGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAgcgc                               <  8:34612/149‑1 (MQ=255)
                                                                                                              aaaGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGGACATGATACCGGTCACGGTGGAGCGCCTGGCGGGTCATGGCGTGCTGGAACCACTTACCCCGCCTGCGGCCCTTGCCGGGCGGCTGGCCAACGGGGCGTGGTCACAgcggc                              >  8:308908/1‑147 (MQ=255)
                                                                                                                         gcgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCaa                   >  2:200796/1‑149 (MQ=255)
                                                                                                                                           gTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCg  >  7:232060/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                          
TGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACA‑AGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCG  >  NC_002947/3762795‑3763080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑