Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,768,947 G→T 9.5% L178L (CTG→CTT)  PP_3330 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,768,9470GT 9.5% 151.4 / 4.4 51L178L (CTG→CTT) PP_3330ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (24/22);  new base T (2/3);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

AATGGCATCAATTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGACTACAGCCGCACCAG  >  NC_002947/3768804‑3769090
                                                                                                                                                |                                                                                                                                                
aaTGGCATCAATTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCGGGATCTATGACCGCGTCGAATTGCTAGGCGGGGCGGCCTCCTTGCTGCACGGCAGCGGCGGGGTGGGGGGCCG‑CCGGAATTTGATCCCCCGGCGGGCAAg                                                                                                                                             >  8:209869/1‑149 (MQ=255)
  tGGCATCAATTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGcc                                                                                                                                           >  2:41387/1‑149 (MQ=255)
     cATCAATTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGt                                                                                                                                         >  8:23586/1‑148 (MQ=255)
      aTCAATTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATTACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTACTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAAATTAATAACCCGGCTGGCAAGCCGt                                                                                                                                         >  2:144273/1‑147 (MQ=255)
      aTCAATTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGt                                                                                                                                         <  1:144273/147‑1 (MQ=255)
      aTCAATTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGa                                                                                                                                       >  6:255845/1‑149 (MQ=255)
         aaTTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACg                                                                                                                                     <  2:269228/148‑1 (MQ=255)
         aaTTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGa                                                                                                                                    <  1:169107/149‑1 (MQ=255)
          aTTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACACGGCTTGCCAGCCgggtgatg                                                                                                                                   <  4:103163/149‑8 (MQ=255)
               cAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGacac                                                                                                                              >  8:104658/1‑149 (MQ=255)
                      gcggcgcggcGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGaa                                                                                                                       <  4:137055/149‑1 (MQ=255)
                           gcggcgcgCCCGGTGGGCGCCTTGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAGCCCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCtt                                                                                                                           <  1:71592/141‑1 (MQ=255)
                           gcggcgcgCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCtt                                                                                                                           >  2:71592/1‑140 (MQ=255)
                                gcgcCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTTGCCAGCCgggtg                                                                                                                                      >  5:55401/1‑120 (MQ=255)
                                gcgcCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTTGCCAGCCgggtg                                                                                                                                      <  6:55401/124‑5 (MQ=255)
                                  gcCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGt                                                                                                          <  3:243435/149‑1 (MQ=255)
                                    ccaccgggCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTa                                                                                                        <  6:243318/144‑1 (MQ=255)
                                       gTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGc                                                                                                     <  5:255845/149‑1 (MQ=255)
                                              ccTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTAc                                                                                              <  3:173138/149‑1 (MQ=255)
                                                  gATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGaca                                                                                          <  7:104658/149‑1 (MQ=255)
                                                  gATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGa                                                                                            <  7:23586/147‑1 (MQ=255)
                                                           ccGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGGCCGCGTCAGTTATGCACGCTACGACACCATGGa                                                                                   <  1:41387/148‑1 (MQ=255)
                                                                 cGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGa                                                                                                                                       <  5:182964/90‑1 (MQ=255)
                                                                 cGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGa                                                                                                                                       >  6:182964/1‑90 (MQ=255)
                                                                   aaTTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGtt                                                                         >  3:170593/1‑149 (MQ=255)
                                                                       gCTCGGCGAGCCATCCTCCTTCCTCAACGGAAGCGACGCTATCAGTAGCAG‑ACTCAATTTCATCACCCGACTTGCAAGCAGTAACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGAAAACATGGAAACGTCGGTTGcc                                                                      >  8:50531/1‑148 (MQ=255)
                                                                        cTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCt                                                                     >  5:71345/1‑148 (MQ=255)
                                                                                 ccGTCCTCCTTACTCAACGGCAGCGGCGCTGTCGTTGGCAG‑CCTCAATTTCATAACCCGGCTTGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATTCACGATACGACACCATGGAAACGTCGGTTGCCTTCAACAAGgc                                                           <  7:50531/149‑1 (MQ=255)
                                                                                      ctcctTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTg                                                       >  3:222471/1‑148 (MQ=255)
                                                                                      ctcctTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGa                                                      <  4:170593/149‑1 (MQ=255)
                                                                                       tccttcctCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTg                                                       >  7:11341/1‑147 (MQ=255)
                                                                                       tccttcctCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTg                                                       <  8:11341/147‑1 (MQ=255)
                                                                                         cttcctCAACGGCAGCAGCACTATCGGTGGCAG‑CCTCAATTTCATCACCCGACTGACAAGCCGTGACGAAGACACATTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATTGAAACGTCGGTTGCATTAAACAAGGCGCTTAaca                                                   >  8:50868/1‑149 (MQ=255)
                                                                                              tCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGc                                              >  3:96431/1‑149 (MQ=255)
                                                                                              tCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGc                                              >  4:106675/1‑149 (MQ=255)
                                                                                                    gCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGc                                        >  3:132267/1‑149 (MQ=255)
                                                                                                    gCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGc                                        >  7:42487/1‑149 (MQ=255)
                                                                                                    gCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGc                                        >  8:255734/1‑149 (MQ=255)
                                                                                                           cgcTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGcc                                 >  7:262147/1‑149 (MQ=255)
                                                                                                             cTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGccac                               <  8:42487/149‑1 (MQ=255)
                                                                                                                    tGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATgcgc                        <  8:262147/149‑1 (MQ=255)
                                                                                                                         g‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTc                   >  5:295136/1‑149 (MQ=255)
                                                                                                                            cTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGa                 <  3:106675/149‑1 (MQ=255)
                                                                                                                            cTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGa                 >  4:80292/1‑149 (MQ=255)
                                                                                                                               aaTTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGACt               <  3:80292/148‑1 (MQ=255)
                                                                                                                               aaTTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGACTa              <  6:295136/149‑1 (MQ=255)
                                                                                                                                     atcaCCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGCACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGACTACAGCCg        >  6:94172/1‑149 (MQ=255)
                                                                                                                                     atcaCCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGACTACAGCCg        >  1:233813/1‑149 (MQ=255)
                                                                                                                                          ccGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGACTACAGc          <  8:317029/142‑1 (MQ=255)
                                                                                                                                          ccGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGACTACAGc          >  7:317029/1‑142 (MQ=255)
                                                                                                                                           cGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGACTACAGCCgcaccag  <  6:71345/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AATGGCATCAATTTGCAGTATGGCGGCGCGGCGCGCCCGGTGGGCGCCTGGATCTATGACCGCGTCGAATTGCTCGGCGGGCCGTCCTCCTTCCTCAACGGCAGCGGCGCTGTCGGTGGCAG‑CCTCAATTTCATCACCCGGCTGGCAAGCCGTGACGAAGACACCTTCGAAGGG‑CGCGTCAGTTATGCACGCTACGACACCATGGAAACGTCGGTTGCCTTCAACAAGGCGCTGAACACGGGCGACGGCCCACGCCACCATGCGCGCCTCGACTACAGCCGCACCAG  >  NC_002947/3768804‑3769090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑