Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,792,941	T→A	6.3%	Y126F (TAC→TTC)	PP_3354 ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,792,941	0	T	A	6.3%	191.2 / 2.5	63	Y126F (TAC→TTC)	PP_3354	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (27/32);  new base A (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CAGGCTGAGATCGCTGCCGGCCTGGGGTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGGCGCGATAAGCCGCCGGGAATCC  >  NC_002947/3792793‑3793089                                                                                                                                                     |                                                                                                                                                      cAGGCTGAGATCGCTGCCGGCCTGGGGTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGgtgt                                                                                                                                                       <  2:254154/149‑1 (MQ=255)   ggCTGAGATCGCTGCCGGCCTGGGGTTCGGTCATGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTAc                                                                                                                                                     <  5:186099/149‑1 (MQ=255)   ggCAGAGATCGCTGCCGGCCTGGGGTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGCCGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGAGGGCGATGCCGGTGTa                                                                                                                                                      >  8:321656/1‑148 (MQ=255)      tGAGATCGCTGCCGGCCTGGGGTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCATGGGCGATGCCGGTGTACATg                                                                                                                                                  >  8:12971/1‑149 (MQ=255)            cGCTGCCGGCCTGGGGTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAg                                                                                                                                            >  8:284730/1‑149 (MQ=255)                 ccGGCCTGGGGTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTg                                                                                                                                       <  1:25838/149‑1 (MQ=255)                  cGGCCTGGGGTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTgg                                                                                                                                      <  3:67750/149‑1 (MQ=255)                    gCCTGGGGTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGtt                                                                                                                                    <  4:155477/149‑1 (MQ=255)                        ggggTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTtgctg                                                                                                                                >  2:125873/1‑149 (MQ=255)                         gggTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTtgctgc                                                                                                                               <  7:284730/149‑1 (MQ=255)                           gTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTAc                                                                                                                                                     >  4:153635/1‑125 (MQ=255)                           gTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTAc                                                                                                                                                     <  3:153635/125‑1 (MQ=255)                           gTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGcc                                                                                                                              >  2:268179/1‑148 (MQ=255)                              cGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGaa                                                                                                                           <  1:268179/148‑1 (MQ=255)                               ggTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGaa                                                                                                                           >  3:103971/1‑147 (MQ=255)                                 tCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGc                                                                                                                       <  3:123260/149‑1 (MQ=255)                                    ggCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCAtc                                                                                                                    <  5:143615/149‑1 (MQ=255)                                       acaTGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACGGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATctct                                                                                                                 >  7:144697/1‑149 (MQ=255)                                          tGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTg                                                                                                               >  5:175572/1‑148 (MQ=255)                                          tGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGc                                                                                                              <  1:125873/149‑1 (MQ=255)                                                gCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACGGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGgcc                                                                                                        <  8:144697/149‑1 (MQ=255)                                                 cAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGgccg                                                                                                       >  2:272427/1‑149 (MQ=255)                                                         caccacTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGc                                                                                               <  1:280927/149‑1 (MQ=255)                                                                 aCGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AAc                                                                                        <  1:14488/147‑1 (MQ=255)                                                                        ccGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCt                                                                               >  5:178694/1‑149 (MQ=255)                                                                         cGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCTTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCtt                                                                              <  7:12971/149‑1 (MQ=255)                                                                         cGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAg                                                                                                >  2:317472/1‑132 (MQ=255)                                                                         cGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAg                                                                                                <  1:317472/132‑1 (MQ=255)                                                                         cGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCtt                                                                              <  3:320567/149‑1 (MQ=255)                                                                               aTAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGTCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGgccgc                                                                        >  8:100385/1‑149 (MQ=255)                                                                               aTAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGAACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGgccgc                                                                        >  7:201372/1‑149 (MQ=255)                                                                               aTAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGAACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGgccgc                                                                        >  7:110014/1‑149 (MQ=255)                                                                                   cgcgcCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAg                                                                    >  7:270009/1‑149 (MQ=255)                                                                                   cgcgcCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAg                                                                    >  5:147486/1‑149 (MQ=255)                                                                                        cTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCg                                                               <  6:147486/149‑1 (MQ=255)                                                                                           cAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCgggg                                                            >  1:116170/1‑149 (MQ=255)                                                                                           cAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGAACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCggg                                                             <  8:201372/148‑1 (MQ=255)                                                                                           cAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGAACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCggg                                                             <  8:110014/148‑1 (MQ=255)                                                                                            aGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGgc                                                           <  2:125979/149‑1 (MQ=255)                                                                                            aGCTCGGCGGATGCAGGGGCGTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGgc                                                           <  7:321656/149‑1 (MQ=255)                                                                                             gCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGgcg                                                          >  8:236524/1‑149 (MQ=255)                                                                                              cTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGgcg                                                          >  3:121858/1‑148 (MQ=255)                                                                                               tCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGc                                                                                                              <  2:236425/96‑1 (MQ=255)                                                                                               tCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCa                                                        >  6:216931/1‑149 (MQ=255)                                                                                                 ggcggATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCAt                                                       <  8:270009/148‑1 (MQ=255)                                                                                                 ggcggATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGACGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCAt                                                       <  6:248014/148‑1 (MQ=255)                                                                                                          aTGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCaa                                                                     <  1:200816/125‑1 (MQ=255)                                                                                                          aTGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCaa                                                                     >  2:200816/1‑125 (MQ=255)                                                                                                             ggCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGgc                                           <  3:278975/148‑1 (MQ=255)                                                                                                                  tCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGgccagc                                       <  6:178694/147‑1 (MQ=255)                                                                                                                        acaGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTTGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCg                                  >  4:86172/1‑146 (MQ=255)                                                                                                                            gTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACa                            >  4:133849/1‑148 (MQ=255)                                                                                                                                gCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGgc                       >  3:91468/1‑149 (MQ=255)                                                                                                                                gCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGgc                       >  8:219001/1‑149 (MQ=255)                                                                                                                                  aCCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACa                            <  5:37387/142‑1 (MQ=255)                                                                                                                                  aCCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACa                            >  6:37387/1‑142 (MQ=255)                                                                                                                                    cGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACTCAACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCAt                                                       <  1:230453/114‑1 (MQ=255)                                                                                                                                    cGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCAt                                                       >  2:230453/1‑113 (MQ=255)                                                                                                                                     gTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGGCGCGAt                  <  4:103971/149‑1 (MQ=255)                                                                                                                                        ggCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGa                              <  2:268268/134‑1 (MQ=255)                                                                                                                                        ggCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAAGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGa                              >  1:268268/1‑134 (MQ=255)                                                                                                                                               ccGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGGCGCGATAAGccgccg         <  6:175572/148‑1 (MQ=255)                                                                                                                                               ccGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGGCGCGATAAGCCGCCgg        <  5:216931/149‑1 (MQ=255)                                                                                                                                               ccGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGGCGCGATAAGCCGCCgg        <  5:165047/149‑1 (MQ=255)                                                                                                                                                   tgtACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCTGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGGCGCGATAAGCCGCCGGGAAt    <  1:272427/149‑1 (MQ=255)                                                                                                                                                   tgtACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGGCGCGATAAGCCGCCGGGAAt    <  2:116170/149‑1 (MQ=255)                                                                                                                                                     tACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGGCGCGATAAGCCGCCGGGAAtcc  >  1:44786/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                      CAGGCTGAGATCGCTGCCGGCCTGGGGTTCGGTCAGGCACATGGTAGGCAGGGTGGCACCACTGACGATGCCCGGCAGATAGCGCGCCTGCAGCTCGGCGGATGCATGGGCTTTCAGGCACAGGTAGGCACCGTGGGCGATGCCGGTGTACATGGCCCAGCCGTGGTTGCTGCCGAACAGCATCTCTTGCAAGGCCGCCTCCAGCACT‑AACGGCAGCCCTTGGCCGCCAAGCGCCGGGGCGCATGCCAGCGACGGCCAGCCACCGTCGACATAGGCGCGATAAGCCGCCGGGAATCC  >  NC_002947/3792793‑3793089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑