Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,806,743 G→A 7.1% L453L (TTG→TTA) ‡ PP_3364 → two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,806,7430GA 7.1% 163.5 / 2.1 56L453L (TTG→TTA) ‡PP_3364two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (25/27);  new base A (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

CGTATTTTAATGATGTGTCTGGTGTGCTGATTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGTGCTGCACAATAGCGAA  >  NC_002947/3806601‑3806887
                                                                                                                                              |                                                                                                                                                
cGTATTTTAATGATGTGTCTGGTGTGCTGATTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGAc                                                                                                                                            <  6:180486/149‑1 (MQ=255)
    ttttAATGATGTGTCTGGTGTGCTGATTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGc                                                                                                                                         <  3:73009/148‑1 (MQ=255)
        aaTGATGTGTCTGGTGTGCTGATTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGa                                                                                                                                     <  7:79658/148‑1 (MQ=255)
                 tCTGGTGTGCTGATTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCaa                                                                                                                           <  8:42117/149‑1 (MQ=255)
                 tCTGGTGTGCTGATTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCaa                                                                                                                           <  7:32065/149‑1 (MQ=255)
                  cTGTTGTGCTGACTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCaa                                                                                                                           <  8:47319/148‑1 (MQ=255)
                        tgCTGATTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTc                                                                                                                    <  3:330149/149‑1 (MQ=255)
                          cTGATTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCt                                                                                                                                       <  8:127474/128‑1 (MQ=255)
                          cTGATTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCt                                                                                                                                       >  7:127474/1‑128 (MQ=255)
                             attattCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCAt                                                                                                               >  8:173405/1‑149 (MQ=255)
                                attCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCt                                                                                                             <  3:104827/148‑1 (MQ=255)
                                            cAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGG‑CGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGtt                                                                                               <  2:166994/149‑1 (MQ=255)
                                             aaCACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGtt                                                                                               >  6:39010/1‑149 (MQ=255)
                                                  ccAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGcc                                                                                                                                 >  5:131360/1‑110 (MQ=255)
                                                  ccAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGcc                                                                                                                                 <  6:131360/110‑1 (MQ=255)
                                                   cAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCTATCTGGAAAGAAACCGTTCACcag                                                                                         >  4:92637/1‑149 (MQ=255)
                                                         aTTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCg                                                                                   >  4:169003/1‑149 (MQ=255)
                                                             cTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTAc                                                                               >  7:205785/1‑149 (MQ=255)
                                                             cTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTAc                                                                               >  5:133504/1‑149 (MQ=255)
                                                              ttGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTAcc                                                                              >  5:308124/1‑149 (MQ=255)
                                                                gATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGgaaa                                                                                                        >  2:228277/1‑121 (MQ=255)
                                                                gATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGgaaa                                                                                                        <  1:228277/121‑1 (MQ=255)
                                                                       ttcCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGa                                                                     >  8:148369/1‑149 (MQ=255)
                                                                               ggaggTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGAcc                                                             <  8:205785/149‑1 (MQ=255)
                                                                               ggaggTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGAcc                                                             <  6:133504/149‑1 (MQ=255)
                                                                                     ggtCGAGACACTCGATTGGGCCGGGGACGCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACcagc                                                                                        >  1:194861/1‑116 (MQ=255)
                                                                                     ggtCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACcagc                                                                                        <  2:194861/116‑1 (MQ=255)
                                                                                       tCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGttt                                                     >  7:325026/1‑149 (MQ=255)
                                                                                         gagaCACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTgg                                                   >  5:201152/1‑149 (MQ=255)
                                                                                              acTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATg                                              >  2:148005/1‑149 (MQ=255)
                                                                                                  gATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCAt                                          <  7:254576/149‑1 (MQ=255)
                                                                                                     tGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCg                                       >  8:22349/1‑149 (MQ=255)
                                                                                                         ccGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCAcc                                   <  3:169003/149‑1 (MQ=255)
                                                                                                             ggACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTAcc                                                                              <  4:206443/102‑1 (MQ=255)
                                                                                                             ggACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTAcc                                                                              >  3:206443/1‑102 (MQ=255)
                                                                                                               aCCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTAtc                             <  8:325026/149‑1 (MQ=255)
                                                                                                                    aaCAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGa                        <  6:308124/149‑1 (MQ=255)
                                                                                                                         gACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCCGGACCGCCAGTTTGGCGATGCGATCCGCGCCGCGATCGTCGAggtgg                   >  8:178512/1‑149 (MQ=255)
                                                                                                                            cTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCAc                                    <  6:282818/129‑1 (MQ=255)
                                                                                                                            cTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCAc                                    >  5:282818/1‑129 (MQ=255)
                                                                                                                            cTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGtgc                >  4:320612/1‑149 (MQ=255)
                                                                                                                            cTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGtgc                >  3:26480/1‑149 (MQ=255)
                                                                                                                             tATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGc                                                                   >  1:40776/1‑97 (MQ=255)
                                                                                                                             tATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGc                                                                   <  2:40776/97‑1 (MQ=255)
                                                                                                                               ctcttgcggggCGTCAGGCGGTCGCCCAACGCGCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCg                                  >  2:13082/6‑128 (MQ=37)
                                                                                                                               ctcttgcggggCGTCAGGCGGTCGCCCAACGCGCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCg                                  <  1:13082/123‑1 (MQ=37)
                                                                                                                                 aCAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGTGCTGca            <  7:148369/148‑1 (MQ=255)
                                                                                                                                 aCAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGTGCTGca            >  6:156501/1‑148 (MQ=255)
                                                                                                                                 aCAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGTGCTGca            >  4:214263/1‑148 (MQ=255)
                                                                                                                                   tgcggggCGTCAGGCGGTCGCCCAACGCGCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGc                                                  >  2:245933/2‑108 (MQ=37)
                                                                                                                                   tgcggggCGTCAGGCGGTCGCCCAACGCGCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGc                                                  <  1:245933/107‑1 (MQ=37)
                                                                                                                                     cggcgCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGTGCTGCACaa         >  5:52823/1‑147 (MQ=255)
                                                                                                                                      ggcgCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGTGCTGCACAATAg      <  3:320612/149‑1 (MQ=255)
                                                                                                                                       gcgcGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGTGCTGCACAATAGc     <  7:173405/149‑1 (MQ=255)
                                                                                                                                       gcgcGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGTGCTGCACAATAGc     <  4:26480/149‑1 (MQ=255)
                                                                                                                                          cgTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGTGCTGCACAATAGCGaa  <  5:39010/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
CGTATTTTAATGATGTGTCTGGTGTGCTGATTATTCCGATGTCGCAACACCCAAGGGATTACTTGATCTTCTTCCGCAAGGAGGTGGTCGAGACACTCGATTGGGCCGGGGACCCGAACAAGACCTATGACAGCGGCGCGTTGGGCGACCGCCTGACGCCCCGCAAGAGCTTCGCCATCTGGAAAGAAACCGTTCACCAGCAGTCGCTACCCTGGACCGAGCAGGACCGCCAGTTTGGCGATGCCATCCGCACCGCTATCGTCGAGGTGGTGCTGCACAATAGCGAA  >  NC_002947/3806601‑3806887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑