Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	338,065	G→T	8.8%	A168A (GCC→GCA)	PP_0280 ←	amino acid ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	338,065	0	G	T	8.8%	177.1 / 6.8	57	A168A (GCC→GCA)	PP_0280	amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (29/23);  new base T (3/2);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

CAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCGCCT  >  NC_002947/337918‑338213                                                                                                                                                    |                                                                                                                                                      cagcagCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAggg                                                                                                                                                      >  7:177356/1‑149 (MQ=255) cagcagCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAggg                                                                                                                                                      >  4:228331/1‑149 (MQ=255)   gcagcTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCGCGGTACTGGCCAGGGCa                                                                                                                                                    >  4:179517/1‑149 (MQ=255)   gcagcTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCa                                                                                                                                                    >  5:84153/1‑149 (MQ=255)        ttGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGc                                                                                                                                               >  5:64457/1‑149 (MQ=255)          gAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGcc                                                                                                                                              <  5:108018/148‑1 (MQ=255)          gAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGc                                                                                                                                               <  8:177356/147‑1 (MQ=255)           aaGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGcca                                                                                                                                             <  6:64457/148‑2 (MQ=255)             gCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                          <  5:171515/149‑1 (MQ=255)             gCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                          <  5:111201/149‑1 (MQ=255)                ttGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCa                                                                                                                                       >  1:168307/1‑149 (MQ=255)                   cACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATc                                                                                                                                     <  6:344894/148‑1 (MQ=255)                      cAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGTGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGa                                                                                                                                 >  6:280374/1‑149 (MQ=255)                      cAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGa                                                                                                                                 <  7:182127/149‑1 (MQ=255)                           tGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑t                                                                                                                           >  6:177141/1‑149 (MQ=255)                             aaCGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTc                                                                                                                         >  1:163419/1‑149 (MQ=255)                                   aTCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCGCGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCt                                                                                                                   <  3:179517/149‑1 (MQ=255)                                    tCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCt                                                                                                                   <  3:228331/148‑1 (MQ=255)                                        cAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGt                                                                                                                 >  3:321640/1‑146 (MQ=255)                                        cAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGt                                                                                                                 <  4:321640/146‑1 (MQ=255)                                          ggTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGCCAGGTAGGTGCGGCCGAGTATGTTACGGGCCATGCCGGAAATTTCCAGAAGGGGAACGTTACTGGCAAGGGACATGCCCTTGAGCATCAGGATCCG‑TGCGTTGCttaccccc                                                                                                            >  8:225478/1‑142 (MQ=255)                                            gatcacccGCCTGCGGCGAATCATCTTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCgg                                                                                                          <  7:225478/142‑1 (MQ=255)                                             aGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGccagta                                                                                                                                          >  6:268939/1‑113 (MQ=255)                                             aGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGccagta                                                                                                                                          <  5:268939/117‑5 (MQ=255)                                                 cacGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGc                                                                                                     >  5:274732/1‑149 (MQ=255)                                                    gCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGa                                                                                                  >  2:17657/1‑149 (MQ=255)                                                             gaagaaGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAg                                                                                         >  6:148385/1‑149 (MQ=255)                                                              aagaagATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAgc                                                                                        <  2:168307/149‑1 (MQ=255)                                                               agaagaTTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCa                                                                                                        <  6:191417/132‑1 (MQ=255)                                                               agaagaTTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCa                                                                                                        >  5:191417/1‑132 (MQ=255)                                                                ttagaTTTCCACCGGCAGTTAGGTGCGGTCGATGATGTTAAGGTCCATGCCGGTCAGTTCCAGCAGTGTCACGTTACTGGCCATGTCACTGGCCTTGAGCATCATGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGAt                                                                                                 <  1:315439/136‑1 (MQ=255)                                                                gaagaTTTCCACAGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCAAGCAGGGTCACGGTACTGTCCAGGGCACTGGACTTGAGAATCAGGATCAC‑TTCGTTGCTGTACGCAGGAAGGCCTAt                                                                                                 >  2:315439/1‑138 (MQ=255)                                                                   gaTTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAggg                                                                                                                                                      >  3:155963/1‑83 (MQ=255)                                                                   gaTTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAggg                                                                                                                                                      <  4:155963/83‑1 (MQ=255)                                                                   gaTTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTg                                                                                   >  3:205809/1‑149 (MQ=255)                                                                     tttCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTgg                                                                                  <  2:163419/148‑1 (MQ=255)                                                                       tCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgcag                                                                               >  5:125492/1‑149 (MQ=255)                                                                       tCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgcag                                                                               >  5:118932/1‑149 (MQ=255)                                                                       tCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgca                                                                                <  4:205809/148‑1 (MQ=255)                                                                         cACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgcagca                                                                             >  8:23589/1‑149 (MQ=255)                                                                         cACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgcagc                                                                              <  6:87727/148‑1 (MQ=255)                                                                               cAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGt                                                                       <  7:23589/149‑1 (MQ=255)                                                                                          gggCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGTCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCAGGATGTAGAACAGCGtt                                                            >  7:55676/1‑149 (MQ=255)                                                                                          gggCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGtt                                                            <  6:84153/149‑1 (MQ=255)                                                                                            gCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGtttt                                                          <  5:177141/149‑1 (MQ=255)                                                                                                      tACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTTCGTTGCTGTACGCCGGCAGGCCGATgcgc                                                                                             <  1:31789/105‑1 (MQ=255)                                                                                                      tACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATgcgc                                                                                             >  2:31789/1‑104 (MQ=255)                                                                                                                         ttCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCg                             >  4:28472/1‑149 (MQ=255)                                                                                                                            cagcagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAgcgc                                     <  4:40928/138‑1 (MQ=255)                                                                                                                            cagcagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAgcgc                                     >  3:40928/1‑138 (MQ=255)                                                                                                                            cagcagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCtt                          >  1:317356/1‑149 (MQ=255)                                                                                                                            cagcagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCt                           >  7:62343/1‑148 (MQ=255)                                                                                                                             agcagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCtt                          >  4:70497/1‑148 (MQ=255)                                                                                                                             agcagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTc                         <  6:274732/149‑1 (MQ=255)                                                                                                                              gcagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTc                         >  5:261257/1‑148 (MQ=255)                                                                                                                                      aCGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCatgat                                                                         >  5:249607/1‑92 (MQ=255)                                                                                                                                      aCGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCatgat                                                                         <  6:249607/92‑1 (MQ=255)                                                                                                                                          tcaaggCCAGTGCCCTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCtt              >  5:64546/5‑147 (MQ=255)                                                                                                                                             tGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAgg          >  6:88977/1‑148 (MQ=255)                                                                                                                                                 cAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGAGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCg     >  7:189770/1‑149 (MQ=255)                                                                                                                                                  aGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCGGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGTGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCGc    >  7:298300/1‑149 (MQ=255)                                                                                                                                                    ggCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACCCGACATGCCCAGCGCCCGTGCCGCTACAATCTCACCTTAAGGGATCGCCt  >  1:116457/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      CAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAC‑TTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCGCCT  >  NC_002947/337918‑338213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑