Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,844,924 A→T 8.9% L237Q (CTG→CAG)  PP_3394 ← 3‑hydroxy‑3‑methylglutaryl‑CoA lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,844,9240AT 8.9% 127.2 / 2.6 45L237Q (CTG→CAG) PP_33943‑hydroxy‑3‑methylglutaryl‑CoA lyase
Reads supporting (aligned to +/- strand):  ref base A (21/20);  new base T (2/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCTTAGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGC  >  NC_002947/3844791‑3845069
                                                                                                                                     |                                                                                                                                                 
gcttAGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAg                                                                                                                                     <  5:110976/148‑1 (MQ=255)
     gTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATcc                                                                                                                               <  1:167643/149‑1 (MQ=255)
      tACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCa                                                                                                                              <  5:147548/149‑1 (MQ=255)
              gggTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTcccc                                                                                                                       >  7:310782/1‑148 (MQ=255)
                gTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCatg                                                                                                                    >  2:209925/1‑147 (MQ=255)
                 tttCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATg                                                                                                                   >  5:95252/1‑149 (MQ=255)
                          gcgcAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCctgctg                                                                                                           >  2:282434/1‑148 (MQ=255)
                                  aaGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGccac                                                                                                  <  6:95252/149‑1 (MQ=255)
                                                 tCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCaa                                                                                   <  8:310782/149‑1 (MQ=255)
                                                   gTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAagg                                                                                 >  1:299505/1‑149 (MQ=255)
                                                     aGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGtt                                                                                         <  6:177034/139‑1 (MQ=255)
                                                     aGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGtt                                                                                         >  5:177034/1‑139 (MQ=255)
                                                      gCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAaggcca                                                                              <  3:143679/149‑1 (MQ=255)
                                                         cATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCA‑CAGGCTGTTGGCCAaggccaggcc                                                                          >  5:95384/1‑149 (MQ=255)
                                                             ctgcaaagcaTGGACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGcccc                                                                        <  4:235856/142‑1 (MQ=255)
                                                               ttGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCggg                                                                     >  5:230528/1‑149 (MQ=255)
                                                                 gcagcaTGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAgg                                                                                              >  8:26102/1‑122 (MQ=255)
                                                                 gcagcaTGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAgg                                                                                              <  7:26102/122‑1 (MQ=255)
                                                                 ccagcaTGAACACCAGGACCTCGGTCACGGTATTACCAGACGCGACAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGATCCCCAATGCCCTGCTGCACCGCCACAAGACTGTTGGCCAAGTCAAGGcccccgc                                                                     >  8:302373/2‑144 (MQ=255)
                                                                  cagcaTGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGgtgt                                                                  >  8:282046/1‑149 (MQ=255)
                                                                         aaCACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGaa                                                           <  6:230528/149‑1 (MQ=255)
                                                                         aaCACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGaa                                                           <  2:75966/149‑1 (MQ=255)
                                                                         aaCACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCACCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGaa                                                           <  1:282434/149‑1 (MQ=255)
                                                                          acacCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAg                                                          <  2:299505/149‑1 (MQ=255)
                                                                           cacCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGt                                                         <  2:295493/149‑1 (MQ=255)
                                                                           cacCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGt                                                         >  4:276183/1‑149 (MQ=255)
                                                                           cacCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGCAAg                                                          >  1:295493/1‑148 (MQ=255)
                                                                                   ccTCGGTCACGGTATTACCAGACGAGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGcc                                                 >  5:271492/1‑149 (MQ=255)
                                                                                       ggTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTg                                              <  6:87079/148‑1 (MQ=255)
                                                                                         tCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTgaga                                           >  1:308431/1‑149 (MQ=255)
                                                                                           aCGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACg                                         >  4:333725/1‑149 (MQ=255)
                                                                                                   aCCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCt                                  <  7:282046/148‑1 (MQ=255)
                                                                                                    ccAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGc                                <  3:305796/149‑1 (MQ=255)
                                                                                                            gcCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTccc                        >  3:36280/1‑149 (MQ=255)
                                                                                                                        cGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCA‑CAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAg           <  6:95384/149‑1 (MQ=255)
                                                                                                                          ggCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGc          <  6:271492/149‑1 (MQ=255)
                                                                                                                           gatgCCTCCCTGGCGGGCCTGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCa         >  4:241385/4‑149 (MQ=255)
                                                                                                                           gCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCa         >  4:73308/1‑149 (MQ=255)
                                                                                                                             aGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCAc        >  8:163090/1‑148 (MQ=255)
                                                                                                                                cTCCCTGGCGGGCCTGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCtt                                               >  7:295086/1‑106 (MQ=255)
                                                                                                                                cTCCCTGGCGGGCCTGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCtt                                               <  8:295086/106‑1 (MQ=255)
                                                                                                                                cTCCCTGGCGGGCCTGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCAtt    <  3:241385/149‑1 (MQ=255)
                                                                                                                                cTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCAtt    <  3:276183/149‑1 (MQ=255)
                                                                                                                                 tCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAACCCCCATTg   >  8:220401/1‑149 (MQ=255)
                                                                                                                                   ccAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCCTTGc  >  4:261534/1‑148 (MQ=255)
                                                                                                                                     |                                                                                                                                                 
GCTTAGTACCTGCCGGGTTTCGATTAGCGCAGGCAAGTCGATGCCGGTGTCGTAGCCCATGCTTTGCAGCATGAACACCAGGTCCTCGGTCACGGTATTACCAGACGCGCCAGGGGCGAACGGGCAGCCTCCCAGGCCCGCCAGGGAGGCATCCAGTTCCCCAATGCCCTGCTGCACCGCCACCAGGCTGTTGGCCAAGGCCAGGCCCCGGGTGTCATGGAAGTGCGCGGCCTTGAGACGGTCGCCTGCAATCTCCCGTACGGCCTGAAGCACCATTGC  >  NC_002947/3844791‑3845069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑