Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,853,771 T→C 11.3% intergenic (+54/+28) PP_3401 → / ← PP_3402 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,853,7710TC11.3% 137.0 / 8.3 53intergenic (+54/+28)PP_3401/PP_3402hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base C (4/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

CCTTCGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAC  >  NC_002947/3853623‑3853918
                                                                                                                                                    |                                                                                                                                                   
ccTTCGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCt                                                                                                                                                     >  2:294140/1‑149 (MQ=255)
ccTTCGTCTGGGATGGGCGGAGTAATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGCCTGCTTATACCCGGCCAGCCt                                                                                                                                                     >  3:246639/1‑149 (MQ=255)
  ttCGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTgg                                                                                                                                                   >  1:346567/1‑149 (MQ=255)
    cttCTGGGATGGGTTGAGTTATCACGTCTTGGTTCGCGAGAAGGTAAAGTGCGGCGACTGTGGCCTGAACGTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTggg                                                                                                                                                  <  4:246639/146‑1 (MQ=255)
    cGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTgg                                                                                                                                                   <  2:192480/147‑1 (MQ=255)
      tCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCt                                                                                                                                                >  8:263692/1‑148 (MQ=255)
          ggATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGAc                                                                                                                                             <  2:22958/147‑1 (MQ=255)
          ggATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCg                                                                                                                                           >  8:117867/1‑149 (MQ=255)
           gATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAGGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgg                                                                                                                                          <  7:232175/149‑1 (MQ=255)
               ggCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGc                                                                                                                                       <  5:331789/148‑1 (MQ=255)
               ggCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCa                                                                                                                                      <  8:268654/149‑1 (MQ=255)
                gCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCAc                                                                                                                                     >  6:342103/1‑149 (MQ=255)
                        aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgcg                                                                                                                             >  8:221681/1‑149 (MQ=255)
                        aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgc                                                                                                                              <  5:286734/148‑1 (MQ=255)
                        aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgc                                                                                                                              <  4:166334/148‑1 (MQ=255)
                         tCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCggg                                                                                                                                         >  5:236722/1‑136 (MQ=255)
                         tCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCggg                                                                                                                                         <  6:236722/136‑1 (MQ=255)
                             ggCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTc                                                                                                                        <  4:19891/149‑1 (MQ=255)
                                   gTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCaa                                                                                                                  >  7:82676/1‑149 (MQ=255)
                                      cgcgAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATc                                                                                                                <  7:117867/148‑1 (MQ=255)
                                         gagaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgggtataa                                                                                                                                    <  6:171751/125‑6 (MQ=255)
                                         gagaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgggtataa                                                                                                                                    >  5:171751/1‑120 (MQ=255)
                                                gAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTgg                                                                                                     <  1:294140/149‑1 (MQ=255)
                                                        ggcgACGGGGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGt                                                                                             <  3:308281/149‑1 (MQ=255)
                                                          cgACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTgg                                                                                           <  5:342103/149‑1 (MQ=255)
                                                            aCGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCt                                                                                         <  8:82676/149‑1 (MQ=255)
                                                              ggTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTAt                                                                                       <  7:221681/149‑1 (MQ=255)
                                                                 ggCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCac                                                                                    <  2:346567/149‑1 (MQ=255)
                                                                    cTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCacgc                                                                                  >  2:270221/1‑148 (MQ=255)
                                                                               gctgGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                      >  3:299911/1‑149 (MQ=255)
                                                                                ctgGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                      <  7:263692/148‑1 (MQ=255)
                                                                                          aaTAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAg                                                           <  3:284268/149‑1 (MQ=255)
                                                                                            tAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                      >  5:112222/1‑136 (MQ=255)
                                                                                            tAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                      <  6:112222/136‑1 (MQ=255)
                                                                                               gCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGa                                                      >  2:177962/1‑149 (MQ=255)
                                                                                                   aTCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGCCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAg                                                  >  5:63783/1‑149 (MQ=255)
                                                                                                   aTCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAg                                                  <  1:270221/149‑1 (MQ=255)
                                                                                                       gcggcgGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCa                                              >  7:203008/1‑149 (MQ=255)
                                                                                                                     cACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAg                                >  6:280340/1‑149 (MQ=255)
                                                                                                                                 tgctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAgcgc                    >  6:274686/1‑149 (MQ=255)
                                                                                                                                   ctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGa                  >  4:293317/1‑149 (MQ=255)
                                                                                                                                   ctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGa                  <  5:274686/149‑1 (MQ=255)
                                                                                                                                    ttATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAg                                <  3:284272/134‑1 (MQ=255)
                                                                                                                                    ttATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAg                                >  4:284272/1‑134 (MQ=255)
                                                                                                                                        aCCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATAACGCAACGCATGCAGCTGATCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTTCAGCGCTACCCCg             >  2:263952/1‑149 (MQ=255)
                                                                                                                                         cccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGt            >  4:191403/1‑149 (MQ=255)
                                                                                                                                          ccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCCTGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  6:254915/1‑149 (MQ=255)
                                                                                                                                          ccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCCCCAGGTTGCCCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  4:228394/1‑149 (MQ=255)
                                                                                                                                          ccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  3:171813/1‑149 (MQ=255)
                                                                                                                                          ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  5:157005/1‑149 (MQ=255)
                                                                                                                                          ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  7:291729/1‑149 (MQ=255)
                                                                                                                                          ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  1:26212/1‑149 (MQ=255)
                                                                                                                                             gCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAg        <  4:299911/149‑1 (MQ=255)
                                                                                                                                                  ccTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCa   >  7:82257/1‑149 (MQ=255)
                                                                                                                                                   cTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAc  <  5:280340/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCTTCGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAC  >  NC_002947/3853623‑3853918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑