Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,853,772 G→A 12.0% intergenic (+55/+27) PP_3401 → / ← PP_3402 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,853,7720GA12.0% 125.6 / 7.6 50intergenic (+55/+27)PP_3401/PP_3402hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base A (4/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

TTCGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAC  >  NC_002947/3853625‑3853918
                                                                                                                                                   |                                                                                                                                                  
ttCGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTgg                                                                                                                                                   >  1:346567/1‑149 (MQ=255)
  cttCTGGGATGGGTTGAGTTATCACGTCTTGGTTCGCGAGAAGGTAAAGTGCGGCGACTGTGGCCTGAACGTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTggg                                                                                                                                                  <  4:246639/146‑1 (MQ=255)
  cGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTgg                                                                                                                                                   <  2:192480/147‑1 (MQ=255)
    tCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCt                                                                                                                                                >  8:263692/1‑148 (MQ=255)
        ggATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGAc                                                                                                                                             <  2:22958/147‑1 (MQ=255)
        ggATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCg                                                                                                                                           >  8:117867/1‑149 (MQ=255)
         gATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAGGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgg                                                                                                                                          <  7:232175/149‑1 (MQ=255)
             ggCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGc                                                                                                                                       <  5:331789/148‑1 (MQ=255)
             ggCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCa                                                                                                                                      <  8:268654/149‑1 (MQ=255)
              gCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCAc                                                                                                                                     >  6:342103/1‑149 (MQ=255)
                      aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgcg                                                                                                                             >  8:221681/1‑149 (MQ=255)
                      aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgc                                                                                                                              <  4:166334/148‑1 (MQ=255)
                      aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgc                                                                                                                              <  5:286734/148‑1 (MQ=255)
                       tCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCggg                                                                                                                                         <  6:236722/136‑1 (MQ=255)
                       tCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCggg                                                                                                                                         >  5:236722/1‑136 (MQ=255)
                           ggCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTc                                                                                                                        <  4:19891/149‑1 (MQ=255)
                                 gTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCaa                                                                                                                  >  7:82676/1‑149 (MQ=255)
                                    cgcgAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATc                                                                                                                <  7:117867/148‑1 (MQ=255)
                                       gagaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgggtataa                                                                                                                                    <  6:171751/125‑6 (MQ=255)
                                       gagaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgggtataa                                                                                                                                    >  5:171751/1‑120 (MQ=255)
                                              gAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTgg                                                                                                     <  1:294140/149‑1 (MQ=255)
                                                      ggcgACGGGGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGt                                                                                             <  3:308281/149‑1 (MQ=255)
                                                        cgACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTgg                                                                                           <  5:342103/149‑1 (MQ=255)
                                                          aCGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCt                                                                                         <  8:82676/149‑1 (MQ=255)
                                                            ggTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTAt                                                                                       <  7:221681/149‑1 (MQ=255)
                                                               ggCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCac                                                                                    <  2:346567/149‑1 (MQ=255)
                                                                  cTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCacgc                                                                                  >  2:270221/1‑148 (MQ=255)
                                                                             gctgGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                      >  3:299911/1‑149 (MQ=255)
                                                                              ctgGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                      <  7:263692/148‑1 (MQ=255)
                                                                                        aaTAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAg                                                           <  3:284268/149‑1 (MQ=255)
                                                                                          tAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                      >  5:112222/1‑136 (MQ=255)
                                                                                          tAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                      <  6:112222/136‑1 (MQ=255)
                                                                                             gCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGa                                                      >  2:177962/1‑149 (MQ=255)
                                                                                                 aTCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGCCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAg                                                  >  5:63783/1‑149 (MQ=255)
                                                                                                 aTCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAg                                                  <  1:270221/149‑1 (MQ=255)
                                                                                                     gcggcgGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCa                                              >  7:203008/1‑149 (MQ=255)
                                                                                                                   cACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAg                                >  6:280340/1‑149 (MQ=255)
                                                                                                                               tgctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAgcgc                    >  6:274686/1‑149 (MQ=255)
                                                                                                                                 ctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGa                  >  4:293317/1‑149 (MQ=255)
                                                                                                                                 ctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGa                  <  5:274686/149‑1 (MQ=255)
                                                                                                                                  ttATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAg                                <  3:284272/134‑1 (MQ=255)
                                                                                                                                  ttATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAg                                >  4:284272/1‑134 (MQ=255)
                                                                                                                                      aCCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATAACGCAACGCATGCAGCTGATCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTTCAGCGCTACCCCg             >  2:263952/1‑149 (MQ=255)
                                                                                                                                       cccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGt            >  4:191403/1‑149 (MQ=255)
                                                                                                                                        ccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCCTGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  6:254915/1‑149 (MQ=255)
                                                                                                                                        ccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCCCCAGGTTGCCCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  4:228394/1‑149 (MQ=255)
                                                                                                                                        ccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  3:171813/1‑149 (MQ=255)
                                                                                                                                        ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  5:157005/1‑149 (MQ=255)
                                                                                                                                        ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  7:291729/1‑149 (MQ=255)
                                                                                                                                        ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  1:26212/1‑149 (MQ=255)
                                                                                                                                           gCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAg        <  4:299911/149‑1 (MQ=255)
                                                                                                                                                ccTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCa   >  7:82257/1‑149 (MQ=255)
                                                                                                                                                 cTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAc  <  5:280340/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAC  >  NC_002947/3853625‑3853918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑