Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,853,778 A→G 12.0% intergenic (+61/+21) PP_3401 → / ← PP_3402 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,853,7780AG12.0% 121.0 / 8.1 51intergenic (+61/+21)PP_3401/PP_3402hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/23);  new base G (4/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

GGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCACGCCTTGGT  >  NC_002947/3853633‑3853926
                                                                                                                                                 |                                                                                                                                                     
ggATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGAc                                                                                                                                                      <  2:22958/147‑1 (MQ=255)
ggATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCg                                                                                                                                                    >  8:117867/1‑149 (MQ=255)
 gATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAGGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgg                                                                                                                                                   <  7:232175/149‑1 (MQ=255)
     ggCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGc                                                                                                                                                <  5:331789/148‑1 (MQ=255)
     ggCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCa                                                                                                                                               <  8:268654/149‑1 (MQ=255)
      gCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCAc                                                                                                                                              >  6:342103/1‑149 (MQ=255)
              aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgcg                                                                                                                                      >  8:221681/1‑149 (MQ=255)
              aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgc                                                                                                                                       <  4:166334/148‑1 (MQ=255)
              aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgc                                                                                                                                       <  5:286734/148‑1 (MQ=255)
               tCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCggg                                                                                                                                                  <  6:236722/136‑1 (MQ=255)
               tCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCggg                                                                                                                                                  >  5:236722/1‑136 (MQ=255)
                   ggCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTc                                                                                                                                 <  4:19891/149‑1 (MQ=255)
                         gTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCaa                                                                                                                           >  7:82676/1‑149 (MQ=255)
                            cgcgAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATc                                                                                                                         <  7:117867/148‑1 (MQ=255)
                               gagaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgggtataa                                                                                                                                             <  6:171751/125‑6 (MQ=255)
                               gagaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgggtataa                                                                                                                                             >  5:171751/1‑120 (MQ=255)
                                      gAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTgg                                                                                                              <  1:294140/149‑1 (MQ=255)
                                              ggcgACGGGGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGt                                                                                                      <  3:308281/149‑1 (MQ=255)
                                                cgACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTgg                                                                                                    <  5:342103/149‑1 (MQ=255)
                                                  aCGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCt                                                                                                  <  8:82676/149‑1 (MQ=255)
                                                    ggTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTAt                                                                                                <  7:221681/149‑1 (MQ=255)
                                                       ggCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCac                                                                                             <  2:346567/149‑1 (MQ=255)
                                                          cTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCacgc                                                                                           >  2:270221/1‑148 (MQ=255)
                                                                     gctgGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                               >  3:299911/1‑149 (MQ=255)
                                                                      ctgGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                               <  7:263692/148‑1 (MQ=255)
                                                                                aaTAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAg                                                                    <  3:284268/149‑1 (MQ=255)
                                                                                  tAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                               <  6:112222/136‑1 (MQ=255)
                                                                                  tAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgc                                                                               >  5:112222/1‑136 (MQ=255)
                                                                                     gCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGa                                                              >  2:177962/1‑149 (MQ=255)
                                                                                         aTCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGCCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAg                                                          >  5:63783/1‑149 (MQ=255)
                                                                                         aTCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAg                                                          <  1:270221/149‑1 (MQ=255)
                                                                                             gcggcgGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCa                                                      >  7:203008/1‑149 (MQ=255)
                                                                                                           cACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAg                                        >  6:280340/1‑149 (MQ=255)
                                                                                                                       tgctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAgcgc                            >  6:274686/1‑149 (MQ=255)
                                                                                                                         ctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGa                          <  5:274686/149‑1 (MQ=255)
                                                                                                                         ctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGa                          >  4:293317/1‑149 (MQ=255)
                                                                                                                          ttATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAg                                        <  3:284272/134‑1 (MQ=255)
                                                                                                                          ttATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAg                                        >  4:284272/1‑134 (MQ=255)
                                                                                                                              aCCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATAACGCAACGCATGCAGCTGATCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTTCAGCGCTACCCCg                     >  2:263952/1‑149 (MQ=255)
                                                                                                                               cccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGt                    >  4:191403/1‑149 (MQ=255)
                                                                                                                                ccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCCTGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt                   >  6:254915/1‑149 (MQ=255)
                                                                                                                                ccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCCCCAG‑GTTGCCCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt                   >  4:228394/1‑149 (MQ=255)
                                                                                                                                ccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt                   >  3:171813/1‑149 (MQ=255)
                                                                                                                                ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt                   >  7:291729/1‑149 (MQ=255)
                                                                                                                                ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt                   >  5:157005/1‑149 (MQ=255)
                                                                                                                                ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt                   >  1:26212/1‑149 (MQ=255)
                                                                                                                                   gCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAg                <  4:299911/149‑1 (MQ=255)
                                                                                                                                        ccTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCa           >  7:82257/1‑149 (MQ=255)
                                                                                                                                         cTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAc          <  5:280340/149‑1 (MQ=255)
                                                                                                                                               tGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGCGTTGACCAGGTCACGACAACTGTTGa                                         <  1:3953/113‑1 (MQ=255)
                                                                                                                                               tGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGa                                         >  2:3953/1‑112 (MQ=255)
                                                                                                                                                 aCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCACGCCTTGGt  <  3:293317/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAG‑GTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCACGCCTTGGT  >  NC_002947/3853633‑3853926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑