Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,942,943 G→A 8.8% A317V (GCG→GTG)  PP_3478 ← secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,942,9430GA 8.8% 191.3 / 7.0 68A317V (GCG→GTG) PP_3478secretion protein
Reads supporting (aligned to +/- strand):  ref base G (32/30);  new base A (2/4);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

CGCTCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGG  >  NC_002947/3942800‑3943086
                                                                                                                                               |                                                                                                                                               
cgCTCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTcc                                                                                                                                            >  4:213849/1‑149 (MQ=255)
 gCTCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTcc                                                                                                                                            >  2:153245/1‑148 (MQ=255)
     aGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACgt                                                                                                                                       <  8:153160/149‑1 (MQ=255)
     aGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACgt                                                                                                                                       <  1:92602/149‑1 (MQ=255)
        aCGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATACGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTc                                                                                                                                    <  8:140680/149‑1 (MQ=255)
          gCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTcgc                                                                                                                                  >  3:3156/1‑149 (MQ=255)
            cGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTACATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTagcg                                                                                                                                 >  1:222408/1‑148 (MQ=255)
             gAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCa                                                                                                                               >  4:251053/1‑149 (MQ=255)
               aGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCGACACGTCGGACGCGGTGCGCATg                                                                                                                             <  5:183880/149‑1 (MQ=255)
                  tgtTTGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTc                                                                                                                          <  2:138985/149‑1 (MQ=255)
                    ttGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCGACACGTCGGACGCGGTGCGCATggccg                                                                                                                         <  6:346860/148‑4 (MQ=38)
                      gggCAATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATGACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGTTCAgg                                                                                                                       <  2:222408/148‑1 (MQ=255)
                       ggCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAgg                                                                                                                       >  5:17673/1‑147 (MQ=255)
                       ggCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAgg                                                                                                                       <  6:17673/147‑1 (MQ=255)
                          cATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGtt                                                                                                                  <  3:213849/149‑1 (MQ=255)
                            ttGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGa                                                                                                                <  3:66340/149‑1 (MQ=255)
                              gCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAg                                                                                                               <  1:86506/148‑1 (MQ=255)
                                    cAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCg                                                                                                        >  1:277225/1‑149 (MQ=255)
                                        gTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTc                                                                                                                                    >  6:161914/1‑117 (MQ=255)
                                        gTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTc                                                                                                                                    <  5:161914/117‑1 (MQ=255)
                                                      aGGTGGCCACCGCCATCACCTCCACTTCCAGCACCACCTCGGGGGTGGACTGGGCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCgg                                                                                      <  4:202309/149‑1 (MQ=255)
                                                       ggTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCggg                                                                                     >  6:342706/1‑149 (MQ=255)
                                                              aCCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAg                                                                              <  1:153245/149‑1 (MQ=255)
                                                                cTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCa                                                                            >  8:4273/1‑149 (MQ=255)
                                                                  ccATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAgg                                                                                                                       >  4:297926/1‑104 (MQ=255)
                                                                   cATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATgg                                                                         >  7:205169/1‑149 (MQ=255)
                                                                   cATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATg                                                                          <  4:3156/148‑1 (MQ=255)
                                                                   cATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATAGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATgg                                                                         >  2:55260/1‑149 (MQ=255)
                                                                       aCCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTGCTGCGACTGCAGCAGCTTCTCGGCCATGCTCACCGCGTCCGACGTGTCGAGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCtt                                                                     <  3:249350/149‑1 (MQ=255)
                                                                       aCCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCtt                                                                     >  6:58195/1‑149 (MQ=255)
                                                                        ccTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGaa                                                                                                <  4:196496/121‑1 (MQ=255)
                                                                        ccTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGaa                                                                                                >  3:196496/1‑121 (MQ=255)
                                                                        ccTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCtt                                                                     >  3:186890/1‑148 (MQ=255)
                                                                         cTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAg                                                                                                               >  7:124530/1‑105 (MQ=255)
                                                                         cTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAg                                                                                                               <  8:124530/105‑1 (MQ=255)
                                                                         cTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAAc                                                                                                   >  7:271694/1‑117 (MQ=255)
                                                                         cTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAAc                                                                                                   <  8:271694/117‑1 (MQ=255)
                                                                            cACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTc                                                                                                         >  4:111343/1‑108 (MQ=255)
                                                                            cACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTc                                                                                                         <  3:111343/108‑1 (MQ=255)
                                                                             aCTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCAt                                                               >  3:225803/1‑149 (MQ=255)
                                                                               ttCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGt                                                             >  2:236535/1‑149 (MQ=255)
                                                                                 ccAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGtt                                                            >  6:307997/1‑148 (MQ=255)
                                                                                      accaccTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAAcgc                                                      >  7:109892/1‑149 (MQ=255)
                                                                                        caccTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCgg                                                    >  3:31325/1‑149 (MQ=255)
                                                                                            tCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGa                                                                   >  2:64747/1‑130 (MQ=255)
                                                                                            tCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGa                                                                   <  1:64747/130‑1 (MQ=255)
                                                                                            tCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGtt                                                >  7:240119/1‑149 (MQ=255)
                                                                                              ggggTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTTAGCATGGTCTTGACCATGTTCAACGCGGTGTTgg                                              >  4:181351/1‑149 (MQ=255)
                                                                                              ggggTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTg                                               <  7:153644/148‑1 (MQ=255)
                                                                                                                cGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAg                              <  8:109892/147‑1 (MQ=255)
                                                                                                                cGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGt                            <  8:205169/149‑1 (MQ=255)
                                                                                                                   cTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTc                                          <  6:294038/132‑1 (MQ=255)
                                                                                                                   cTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTc                                          >  5:294038/1‑132 (MQ=255)
                                                                                                                          agcTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTgcg                  <  4:186890/149‑1 (MQ=255)
                                                                                                                               ctcGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCAt               <  3:251053/147‑1 (MQ=255)
                                                                                                                                  ggCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGAc            >  1:200775/1‑147 (MQ=255)
                                                                                                                                   gCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATg                                                              >  6:81984/1‑96 (MQ=255)
                                                                                                                                   gCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATg                                                              <  5:81984/96‑1 (MQ=255)
                                                                                                                                    ccATGCGCGACACGTCGGACGCGGTGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCa                               <  7:290822/126‑1 (MQ=38)
                                                                                                                                    ccATGCGCGACACGTCGGACGCGGTGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCa                               >  8:290822/1‑126 (MQ=38)
                                                                                                                                     cATGCGCGACACGTCGGACGCGGTGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGt                                                             >  6:13880/1‑95 (MQ=25)
                                                                                                                                     cATGCGCGACACGTCGGACGCGGTGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGt                                                             <  5:13880/95‑1 (MQ=25)
                                                                                                                                      aTGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTc      <  5:307997/149‑1 (MQ=255)
                                                                                                                                         cgcACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTg   >  6:323287/1‑149 (MQ=255)
                                                                                                                                         cgcACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTg   <  5:58195/149‑1 (MQ=255)
                                                                                                                                         cgcACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTg   >  5:101512/1‑149 (MQ=255)
                                                                                                                                         cgcACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTg   <  4:31325/149‑1 (MQ=255)
                                                                                                                                          gcACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTgg  <  1:236535/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CGCTCAGCACGCCGAAGGTGTTGGGCCATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGG  >  NC_002947/3942800‑3943086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑