Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 344,032 A→G 8.1% intergenic (+121/+68) PP_5440 → / ← PP_t72 hypothetical protein/tRNA‑Phe

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947344,0320AG 8.1% 133.4 / 2.7 49intergenic (+121/+68)PP_5440/PP_t72hypothetical protein/tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base A (19/26);  new base G (2/2);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

TCTTCGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGAC  >  NC_002947/343887‑344178
                                                                                                                                                 |                                                                                                                                                   
tcTTCGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACaaaaaa                                                                                                                                                  >  1:63831/1‑149 (MQ=255)
  ttCGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAAcc                                                                                                                                                >  4:189555/1‑149 (MQ=255)
   tCGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAAccc                                                                                                                                               >  5:104549/1‑149 (MQ=255)
         cATGTCGGACGATTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCAc                                                                                                                                           >  3:216866/1‑147 (MQ=255)
           tGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAg‑g                                                                                                                                      <  6:125558/149‑1 (MQ=255)
               ggACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCggg                                                                                                                                  >  6:330383/1‑149 (MQ=255)
                 aCGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGtt                                                                                                                                >  1:88327/1‑149 (MQ=255)
                     gTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCtt                                                                                                                            <  1:300796/149‑1 (MQ=255)
                     gTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCtt                                                                                                                            <  3:140183/149‑1 (MQ=255)
                        aGGTTTGTCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAGAAACCCGC‑CTAGTGCGGGTTTTttgtt                                                                                                                         <  7:122659/149‑1 (MQ=39)
                        aGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCttgtt                                                                                                                         <  6:216942/149‑1 (MQ=255)
                        aGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCttgt                                                                                                                          <  2:63831/148‑1 (MQ=255)
                         ggTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGAGTGCATGGCTAGCCTTCAGGTGGCGTTGTGAGGGCTCGTGGTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCttgttg                                                                                                                        <  7:243341/149‑1 (MQ=255)
                          gTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTtgttgt                                                                                                                       <  4:216866/149‑1 (MQ=255)
                           tttGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCtt                                                                                                                            <  7:293413/143‑1 (MQ=255)
                           tttGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCtt                                                                                                                            >  8:293413/1‑143 (MQ=255)
                               gCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCt                                                                                                                   <  7:231341/148‑1 (MQ=255)
                                cccGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTc                                                                                                                 <  8:289380/149‑1 (MQ=255)
                                  cGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCgg                                                                                                               <  3:180576/149‑1 (MQ=255)
                                              aTGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCaa                                                                                                   >  3:89239/1‑149 (MQ=255)
                                                  cTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTTTTCTTCGACGCCGACTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑TCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAaccac                                                                                               <  4:237876/149‑1 (MQ=255)
                                                  cTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAaccac                                                                                               <  5:330383/149‑1 (MQ=255)
                                                      gggTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAg                                                                                            >  4:170166/1‑148 (MQ=255)
                                                            ctcGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCtt                                                                                      >  7:17387/1‑148 (MQ=255)
                                                               gATTGCATGGCTAGCCTTCAGGGTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGACGCTTGaa                                                                                   <  6:184688/148‑1 (MQ=255)
                                                                        gCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGccc                                                                         <  2:88327/149‑1 (MQ=255)
                                                                         cTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCa                                                                        <  2:169913/149‑1 (MQ=255)
                                                                           aGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCa                                                                        >  4:235204/1‑147 (MQ=255)
                                                                             ccTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCttg                                                                                                                           <  8:55379/94‑1 (MQ=255)
                                                                             ccTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCttg                                                                                                                           >  7:55379/1‑94 (MQ=255)
                                                                                   ggTTGCGTTGGGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGt                                                              <  7:213193/149‑1 (MQ=255)
                                                                                            gtgAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGTAGTCGAAc                                                         >  3:220191/1‑145 (MQ=255)
                                                                                            gtgAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAAc                                                         <  4:220191/145‑1 (MQ=255)
                                                                                               aGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGa                                                   <  8:17387/148‑1 (MQ=255)
                                                                                                    tcGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAg                                             >  7:250550/1‑149 (MQ=255)
                                                                                                    tcGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGAACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAg                                             >  1:91123/1‑149 (MQ=255)
                                                                                                     cGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAg                                             >  2:167197/1‑148 (MQ=255)
                                                                                                      gtgtTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGa                                           >  8:27706/1‑149 (MQ=255)
                                                                                                            ttcGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTc                                      >  3:320772/1‑148 (MQ=255)
                                                                                                              cGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGAtttt                                       <  1:137027/145‑1 (MQ=255)
                                                                                                               gACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATc                                  <  4:89239/149‑1 (MQ=255)
                                                                                                                  gccgccTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCctc                               <  3:189555/149‑1 (MQ=255)
                                                                                                                  gccgccTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCctc                               <  3:235204/149‑1 (MQ=255)
                                                                                                                       cTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGctc                          >  8:215926/1‑149 (MQ=255)
                                                                                                                                       caGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATc          <  8:250550/149‑1 (MQ=255)
                                                                                                                                         gACAACAAGAAACCCGC‑CTAGTGCGGGTTTTTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTg        >  3:230559/1‑149 (MQ=39)
                                                                                                                                          acaacaAGAAACCCGC‑CTAGTGCGGGTTTTTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATcc                                 <  3:207019/123‑1 (MQ=38)
                                                                                                                                          acaacaAGAAACCCGC‑CTAGTGCGGGTTTTTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATcc                                 >  4:207019/1‑123 (MQ=38)
                                                                                                                                          acaacaAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTgg       >  6:89322/1‑149 (MQ=255)
                                                                                                                                            aacaaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCGCTACCGACTGAGCTATCTGGGc     <  7:215926/149‑1 (MQ=255)
                                                                                                                                                 aaaaCCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGcgac  >  2:311511/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCTTCGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGTTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGAC  >  NC_002947/343887‑344178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑