Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,027,687 C→G 9.1% G248A (GGC→GCC)  PP_3552 ← two‑component system sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,027,6870CG 9.1% 129.9 / 4.0 44G248A (GGC→GCC) PP_3552two‑component system sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (20/20);  new base G (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

CTGCTCGCTGATACGCTGCAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAGGTCTCACGGATCGCCCGGCTGGCGATTT  >  NC_002947/4027548‑4027834
                                                                                                                                              |                                                                                                                                                   
cTGCTCGCTGATACGCTGCAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGc                                                                                                                                              >  4:224090/1‑147 (MQ=255)
     cGCTGATACGCTGCAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCtcg                                                                                                                                       <  1:229761‑M2/149‑4 (MQ=255)
      gCTGATACGCTGCAGGCCCTGGCTTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCtcgc                                                                                                                                      >  2:83712‑M2/1‑145 (MQ=255)
                tGCAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGc                                                                                                                            >  7:223029/1‑149 (MQ=255)
                  cAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGc                                                                                                                            >  1:120966/1‑147 (MQ=255)
                        cTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCggg                                                                                                                    <  8:84804/149‑1 (MQ=255)
                           gCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCAc                                                                                                                 >  2:234699/1‑149 (MQ=255)
                                cccGCTCGTGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGAGCTGGCCTCGGGCA‑cgcccacgagatcaatcag                                                                                                                        >  1:264183‑M2/1‑120 (MQ=255)
                                cccGCTCGTGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGAGCTGGCCTCGGGCA‑cgcccacgagatcaatcag                                                                                                                        <  2:264183‑M2/139‑20 (MQ=255)
                                cccGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTgc                                                                                                            <  3:224090/149‑1 (MQ=255)
                                cccGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTCACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTgc                                                                                                            <  1:6471/149‑1 (MQ=255)
                                    cTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGAGCTGGCCTCGGGCA‑cgcccacgagatcaatcagccactgg                                                                                                                 <  6:276834‑M2/142‑27 (MQ=255)
                                    cTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGAGCTGGCCTCGGGCA‑cgcccacgagatcaatcagccactgg                                                                                                                 >  5:276834‑M2/1‑116 (MQ=255)
                                      cgcgcgCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAgg                                                                                                      >  7:171726/1‑149 (MQ=255)
                                            ccTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTcc                                                                                                <  2:69007/149‑1 (MQ=255)
                                                gggATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTgcg                                                                                            <  2:120966/149‑1 (MQ=255)
                                                  gATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAg                                                                                                                         >  2:142607/1‑118 (MQ=255)
                                                  gATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAg                                                                                                                         <  1:142607/118‑1 (MQ=255)
                                                           ccAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATgcg                                                                                  >  4:103172/1‑148 (MQ=255)
                                                            cAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATgcgc                                                                                 >  6:342516/1‑148 (MQ=255)
                                                                cccTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGt                                                                            <  5:342516/149‑1 (MQ=255)
                                                                  cTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGa                                                                          >  4:281892/1‑149 (MQ=255)
                                                                  cTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGa                                                                          >  6:305617/1‑149 (MQ=255)
                                                                        gTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTcgc                                                                    <  3:281892/149‑1 (MQ=255)
                                                                        gTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTcgc                                                                    <  5:305617/149‑1 (MQ=255)
                                                                            cGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGAt                                                                         <  5:248644/140‑1 (MQ=255)
                                                                            cGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGAt                                                                         >  6:248644/1‑140 (MQ=255)
                                                                              cTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGaca                                                              <  8:223029/149‑1 (MQ=255)
                                                                                   tGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCt                                                          >  8:255522/1‑148 (MQ=255)
                                                                                       gTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCCGAAGGTGAGTTCGCGGGACACGCTGacca                                                     >  4:204263/1‑149 (MQ=255)
                                                                                          ggCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGaccacc                                                   <  7:255522/148‑1 (MQ=255)
                                                                                          ggCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGaccacc                                                   <  1:234699/148‑1 (MQ=255)
                                                                                            cATAGTTGACCACCGCGGCCAGTGGCTGATTGATCCTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTc                                                 >  1:221492/1‑149 (MQ=255)
                                                                                             aTAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTcca                                               >  1:323678/1‑149 (MQ=255)
                                                                                             aTAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTcca                                               >  1:130891/1‑149 (MQ=255)
                                                                                             aTAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTcca                                               >  2:232601/1‑149 (MQ=255)
                                                                                                   gACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCaccacc                                          <  6:323548/148‑1 (MQ=255)
                                                                                                        ccGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACg                                                            <  6:347953/125‑1 (MQ=255)
                                                                                                        ccGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACg                                                            >  5:347953/1‑125 (MQ=255)
                                                                                                           cgGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGgtg                                 <  3:103172/149‑1 (MQ=255)
                                                                                                           cgGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGgtg                                 <  2:229761/149‑1 (MQ=255)
                                                                                                              ccAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAg                              >  7:160762/1‑149 (MQ=255)
                                                                                                                                tcGTGGGCGA‑‑TGCCCGAGGCCAGCtcgcccacgagatcaatcagccactggccgcggtggtcaactatgccaacgccagtcagcgttacctgcagggcctggaacgtgatccccaggcgcgcgagcgggtaggccagggcctgcagcg              <  6:64795‑M2/148‑125 (MQ=255)
                                                                                                                                  gTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAGGTCTCACGGATCGc                <  2:323678/144‑1 (MQ=255)
                                                                                                                                              cccGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAGGTCTCACGGATCGCCAGCCGGGCGAtcc  <  2:130891/148‑3 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CTGCTCGCTGATACGCTGCAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAGGTCTCACGGATCGCCCGGCTGGCGATTT  >  NC_002947/4027548‑4027834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑