| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,027,691 | A→C | 8.5% | S247A (TCG→GCG) | PP_3552 ← | two‑component system sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,027,691 | 0 | A | C | 8.5% | 132.9 / 5.3 | 47 | S247A (TCG→GCG) | PP_3552 | two‑component system sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base A (21/22); new base C (2/2); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01 | |||||||||||
CTGCTCGCTGATACGCTGCAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAGGTCTCACGGATCGCCCGGCTGGCGATTTCGAA > NC_002947/4027548‑4027838 | cTGCTCGCTGATACGCTGCAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGc > 4:224090/1‑147 (MQ=255) cGCTGATACGCTGCAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCtcg < 1:229761‑M2/149‑4 (MQ=255) gCTGATACGCTGCAGGCCCTGGCTTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCtcgc > 2:83712‑M2/1‑145 (MQ=255) tGCAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGc > 7:223029/1‑149 (MQ=255) cAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGc > 1:120966/1‑147 (MQ=255) cTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCggg < 8:84804/149‑1 (MQ=255) gCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCAc > 2:234699/1‑149 (MQ=255) cccGCTCGTGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGAGCTGGCCTCGGGCA‑cgcccacgagatcaatcag > 1:264183‑M2/1‑120 (MQ=255) cccGCTCGTGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGAGCTGGCCTCGGGCA‑cgcccacgagatcaatcag < 2:264183‑M2/139‑20 (MQ=255) cccGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTgc < 3:224090/149‑1 (MQ=255) cccGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTCACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTgc < 1:6471/149‑1 (MQ=255) cTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGAGCTGGCCTCGGGCA‑cgcccacgagatcaatcagccactgg < 6:276834‑M2/142‑27 (MQ=255) cTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGAGCTGGCCTCGGGCA‑cgcccacgagatcaatcagccactgg > 5:276834‑M2/1‑116 (MQ=255) cgcgcgCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAgg > 7:171726/1‑149 (MQ=255) ccTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTcc < 2:69007/149‑1 (MQ=255) gggATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTgcg < 2:120966/149‑1 (MQ=255) gATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAg > 2:142607/1‑118 (MQ=255) gATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAg < 1:142607/118‑1 (MQ=255) ccAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATgcg > 4:103172/1‑148 (MQ=255) cAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATgcgc > 6:342516/1‑148 (MQ=255) cccTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGt < 5:342516/149‑1 (MQ=255) cTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGa > 4:281892/1‑149 (MQ=255) cTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGa > 6:305617/1‑149 (MQ=255) gTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTcgc < 5:305617/149‑1 (MQ=255) gTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTcgc < 3:281892/149‑1 (MQ=255) cGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGAt < 5:248644/140‑1 (MQ=255) cGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGAt > 6:248644/1‑140 (MQ=255) cTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGaca < 8:223029/149‑1 (MQ=255) tGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCt > 8:255522/1‑148 (MQ=255) gTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCCGAAGGTGAGTTCGCGGGACACGCTGacca > 4:204263/1‑149 (MQ=255) ggCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGaccacc < 7:255522/148‑1 (MQ=255) ggCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGaccacc < 1:234699/148‑1 (MQ=255) cATAGTTGACCACCGCGGCCAGTGGCTGATTGATCCTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTc > 1:221492/1‑149 (MQ=255) aTAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTcca > 1:130891/1‑149 (MQ=255) aTAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTcca > 2:232601/1‑149 (MQ=255) aTAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTcca > 1:323678/1‑149 (MQ=255) gACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCaccacc < 6:323548/148‑1 (MQ=255) ccGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACg < 6:347953/125‑1 (MQ=255) ccGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACg > 5:347953/1‑125 (MQ=255) cgGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGgtg < 2:229761/149‑1 (MQ=255) cgGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGgtg < 3:103172/149‑1 (MQ=255) ccAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAg > 7:160762/1‑149 (MQ=255) tcGTGGGCGA‑‑TGCCCGAGGCCAGCtcgcccacgagatcaatcagccactggccgcggtggtcaactatgccaacgccagtcagcgttacctgcagggcctggaacgtgatccccaggcgcgcgagcgggtaggccagggcctgcagcg < 6:64795‑M2/148‑125 (MQ=255) gTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAGGTCTCACGGATCGc < 2:323678/144‑1 (MQ=255) cccGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAGGTCTCACGGATCGCCAGCCGGGCGAtcc < 2:130891/148‑3 (MQ=255) gAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAGGTCTCACGGATCGCCCGGCTGGCGATTTCGaa < 1:247560/149‑1 (MQ=255) gAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAGGTCTCACGGATCGCCCGGCTGGCGATTTCGaa > 2:247560/1‑149 (MQ=255) | CTGCTCGCTGATACGCTGCAGGCCCTGGCCTACCCGCTCGCGCGCCTGGGGATCACGTTCCAGGCCCTGCAGGTAACGCTGACTGGCGTTGGCATAGTTGACCACCGCGGCCAGTGGCTGATTGAT‑CTCGTGGGCGA‑‑TGCCCGAGGCCAGCTCGCCCAGGGTAGCGAGGCGGGCACTGTGCGCCAGGCTTTCCTGCGCTTCGATGCGCAAGGTGATGTCGCGGGACACGCTGACCACCTCCACCACCGCACCGGTGTAGGTCTCACGGATCGCCCGGCTGGCGATTTCGAA > NC_002947/4027548‑4027838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |