Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,042,321	C→A	6.9%	L137I (CTC→ATC) ‡	PP_3563 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,042,321	0	C	A	6.9%	182.7 / 3.6	58	L137I (CTC→ATC) ‡	PP_3563	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (24/30);  new base A (2/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

CCTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCGGGGTCGC  >  NC_002947/4042179‑4042469                                                                                                                                                |                                                                                                                                                     ccTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCg                                                                                                                                                <  6:137558/149‑1 (MQ=255)  cTGTCGGTGCCACGCGACGCACGCGCCGGGATGCATGCCATCGAGGAAAGCTTCTGCCAGGTCGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCgg                                                                                                                                               <  6:243674/149‑1 (MQ=255)     tCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGgc                                                                                                                                              <  7:6497/147‑1 (MQ=255)       ggggCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGCAAGCATCTGCCACGGGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGAGCAGCGgccgt                                                                                                                                           <  1:176300/148‑2 (MQ=255)        gggCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGAGCAGCGgccgtgc                                                                                                                                         <  4:284031/149‑4 (MQ=255)           ccACTCGACGCACGCGCCGCCATGCATGCCATCGATGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCTTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTggg                                                                                                                                      <  3:100241/149‑1 (MQ=255)               gcgACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGAc                                                                                                                                  >  8:81907/1‑149 (MQ=255)                          gccgccATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGcc                                                                                                                         >  7:62500/1‑147 (MQ=255)                                  gcatgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGa                                                                                                                       <  5:345584/141‑1 (MQ=255)                                  gcatgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGa                                                                                                                       >  6:345584/1‑141 (MQ=255)                                  gcatgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAg                                                                                                                >  3:69247/1‑148 (MQ=255)                                   catgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACGGag                                                                                                              <  7:108626/149‑2 (MQ=255)                                    atgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGAc                                                                                                                  <  5:25182/144‑1 (MQ=255)                                    atgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGAc                                                                                                                  >  6:25182/1‑144 (MQ=255)                                       ccATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGtg                                                                                                          <  3:321919/149‑1 (MQ=255)                                         aTCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGAATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGtgc                                                                                                         >  1:76056/1‑149 (MQ=255)                                          tCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCCTCCGCCTGGAGGGCGGCGCCTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCGTGGGCGACTGGCAGGCCGAGGGTGGGAAGGtgctg                                                                                                       >  6:211487/1‑149 (MQ=255)                                               gAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCag                                                                                                    >  2:295663/1‑147 (MQ=255)                                                aaaGCGTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGGGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCaggag                                                                                                 <  8:237752/149‑1 (MQ=255)                                                 aaGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCaggagg                                                                                                <  8:62500/149‑1 (MQ=255)                                                       cTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAAc                                                                                           <  2:56846/148‑1 (MQ=255)                                                         gCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAgg                                                                                                                     <  8:120049/120‑1 (MQ=255)                                                         gCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAgg                                                                                                                     >  7:120049/1‑120 (MQ=255)                                                           cAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGc                                                                                                                                     <  4:36844/102‑1 (MQ=255)                                                           cAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGc                                                                                                                                     >  3:36844/1‑102 (MQ=255)                                                              gtggtgCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAAcgcc                                                                                        >  4:84323/1‑144 (MQ=255)                                                              gtggtgCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAAcgcc                                                                                        <  3:84323/144‑1 (MQ=255)                                                              gtggtgCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCcgcgcg                                                                                   >  8:263827/1‑149 (MQ=255)                                                                      cGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGc                                                                           >  2:161972/1‑149 (MQ=255)                                                                        aCCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCa                                                                         <  5:4282/149‑1 (MQ=255)                                                                           cGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGc                                                                                                                                     >  1:153769/1‑86 (MQ=255)                                                                           cGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGc                                                                                                                                     <  2:153769/86‑1 (MQ=255)                                                                             cAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGt                                                                             <  6:100692/140‑1 (MQ=255)                                                                             cAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGt                                                                             >  5:100692/1‑140 (MQ=255)                                                                              aaTTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCaa                                                                   <  4:112804/149‑1 (MQ=255)                                                                               aTTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAAt                                                                  <  8:293037/149‑1 (MQ=255)                                                                                   gtcg‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATgcgg                                                              <  1:295663/149‑1 (MQ=255)                                                                                   gtcg‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATgcgg                                                              >  7:5182/1‑149 (MQ=255)                                                                                   gtcg‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATgcg                                                               <  1:135932/148‑1 (MQ=255)                                                                                          atCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCggcggc                                                          >  2:44016/1‑147 (MQ=255)                                                                                            cGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGcggcgg                                                           <  4:51399/144‑1 (MQ=255)                                                                                            cGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGcggcgg                                                           >  3:51399/1‑144 (MQ=255)                                                                                             ggCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCAAGcc                                                     >  7:298578/1‑149 (MQ=255)                                                                                                 tCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGttt                                                 <  4:69247/149‑1 (MQ=255)                                                                                                              ccGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGccgccccgc                                    >  8:109869/1‑149 (MQ=255)                                                                                                              ccGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGccgccccgc                                    >  6:206431/1‑149 (MQ=255)                                                                                                               cgcgACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGccgccccgc                                    >  2:209555/1‑148 (MQ=255)                                                                                                                   aCTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACgg                                                                      >  5:131337/1‑110 (MQ=255)                                                                                                                   aCTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACgg                                                                      <  6:131337/110‑1 (MQ=255)                                                                                                                          cATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCa                                  >  7:131018/1‑139 (MQ=255)                                                                                                                          cATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCa                                  <  8:131018/139‑1 (MQ=255)                                                                                                                          cATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGa                        <  5:206431/149‑1 (MQ=255)                                                                                                                               tGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGcaca                   <  7:263827/149‑1 (MQ=255)                                                                                                                                  ccaggcGGCCGCTGAGCAGCGGCCGTCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTg                >  5:148488/6‑149 (MQ=255)                                                                                                                                     ggcGGCCGCTGAGCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTg             >  8:227881/3‑149 (MQ=255)                                                                                                                                      aCGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTgg            <  1:44016/149‑1 (MQ=255)                                                                                                                                           cGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCggg       <  3:149621/149‑1 (MQ=255)                                                                                                                                           cGCTGCTCAGCGGCAGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCggg       <  8:298578/149‑1 (MQ=255)                                                                                                                                                ctCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCGGGGTcgc  <  1:161972/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     CCTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCG‑ATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCGGGGTCGC  >  NC_002947/4042179‑4042469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑