Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,049,910 C→G 11.6% intergenic (+43/‑58) quiA → / → oprB‑III quinate dehydrogenase/carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,049,9100CG11.6% 113.4 / 7.4 43intergenic (+43/‑58)quiA/oprB‑IIIquinate dehydrogenase/carbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base C (16/22);  new base G (2/3);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

CGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGAC  >  NC_002947/4049765‑4050050
                                                                                                                                                 |                                                                                                                                            
cGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGgcc                                                                                                                                            <  2:110589/148‑1 (MQ=255)
 gTCTCGCCGAAGACCTGCAAGCAGTACGTGGTGATCACTGCTTGCGGCGCCCGCCAGTCGACTGACCGTGTCGACTATGTGATTTCTTACGCTCTGCCGTTATACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCt                                                                                                                                          <  2:164347/149‑1 (MQ=39)
 gTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCt                                                                                                                                          <  6:187108/149‑1 (MQ=255)
      gCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAgg                                                                                                                                     >  6:344523/1‑149 (MQ=255)
        cGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTACGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAg                                                                                                                                   >  6:282002/1‑149 (MQ=255)
           aGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAGCgg                                                                                                                                <  5:180354/149‑1 (MQ=255)
            gACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAGCggg                                                                                                                               <  4:7299/149‑1 (MQ=255)
            gACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCggg                                                                                                                               <  8:12248/149‑1 (MQ=255)
            gACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCggg                                                                                                                               <  5:344523/149‑1 (MQ=255)
                 gCAAGCAGTACGTTGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGTGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGc                                                                                                                          <  8:321512/149‑1 (MQ=255)
                     gCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCcgc                                                                                                                      <  2:97691/149‑1 (MQ=255)
                                   gcACTGCGGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACAATGTGATTTCTTACGCTCTGCCGTAAAACCGCGGCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCaaa                                                                                                        <  8:90174/148‑1 (MQ=255)
                                       tgctgGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCg                                                                                                                                 <  3:195301/120‑1 (MQ=255)
                                       tgctgGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCg                                                                                                                                 >  4:195301/1‑120 (MQ=255)
                                        gctgGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCACCTACAACGCCCCTGTAGGAGCGGGCGTGCCCGCGAATGCCCCgacaagacaa                                                                                                   >  8:44128/1‑149 (MQ=255)
                                        actgGCGGGCGCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACCAGGCCCCGGTAGGCGCGGGCTTGCCCGCGAAGGCCCCTCAAAGACaa                                                                                                   >  1:92224/2‑149 (MQ=38)
                                             cggcgCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCga                                                                                              <  5:282002/149‑1 (MQ=255)
                                                       cAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTaa                                                                                    <  7:249291/149‑1 (MQ=255)
                                                             aCTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTAAAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCAt                                                                              >  5:129495/1‑149 (MQ=255)
                                                                 aCCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGc                                                                                                                              <  6:228306/97‑1 (MQ=255)
                                                                 aCCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGc                                                                                                                              >  5:228306/1‑97 (MQ=255)
                                                                        cGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGc                                                                   <  7:110959/149‑1 (MQ=255)
                                                                               gtgATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTAAAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCAccc                                                            >  3:134208/1‑149 (MQ=255)
                                                                                tgATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACCAGGCCCCTGTAGGAGCGGGCTTGCCCCGGCATGGCCCTAAAACACAACCCGAGATTCAGGAATGCCATGCGCCATTCGCaagccccc                                                           >  8:271024/1‑142 (MQ=37)
                                                                                          cGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCctgctg                                                 <  7:44128/149‑1 (MQ=255)
                                                                                           gCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCaaa                                                                                                        <  8:71008/93‑1 (MQ=255)
                                                                                           gCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCaaa                                                                                                        >  7:71008/1‑93 (MQ=255)
                                                                                                 ccttAAAACCGCGGCGCCTGCTTCGCGGGCAAGCCCGCTCATACAAGGCCCCTGTCGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGctggcc                                           <  6:51198/145‑1 (MQ=255)
                                                                                                   gTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGctggccctg                                        <  7:66248/149‑1 (MQ=255)
                                                                                                        cccGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTggccagggccag                                   <  7:250471/148‑1 (MQ=255)
                                                                                                               cGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCAGGGCCAgcagcagg                            <  4:134208/149‑3 (MQ=255)
                                                                                                                        cgagggCAAGCCCGTACCTACACGGCCCCTGGCGTAGGGGGGTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGc                    <  5:33495/145‑1 (MQ=255)
                                                                                                                          cgGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAg                 <  3:215874/149‑1 (MQ=255)
                                                                                                                                aGCCCGCTCCTACAGGGGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGcgacga           >  2:139257/1‑149 (MQ=255)
                                                                                                                                  cccGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGAtc         >  2:29895/1‑149 (MQ=255)
                                                                                                                                  cccGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGAtc         >  1:184061/1‑149 (MQ=255)
                                                                                                                                   ccGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGAtct        <  6:129495/149‑1 (MQ=255)
                                                                                                                                    cGCTCCTACAGGGGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCa                                    <  2:58877/120‑1 (MQ=255)
                                                                                                                                    cGCTCCTACAGGGGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCa                                    >  1:58877/1‑120 (MQ=255)
                                                                                                                                     gCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCa      >  1:41628/1‑149 (MQ=255)
                                                                                                                                     gCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCa      <  2:184061/149‑1 (MQ=255)
                                                                                                                                     gCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCa      >  2:113444/1‑149 (MQ=255)
                                                                                                                                        ccTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGa   >  5:232665/1‑149 (MQ=255)
                                                                                                                                         cTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGAc  >  5:271925/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
CGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGAC  >  NC_002947/4049765‑4050050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑