Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,050,675 C→T 9.6% G236G (GGC→GGT)  oprB‑III → carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,050,6750CT 9.6% 173.5 / 6.4 62G236G (GGC→GGT) oprB‑IIIcarbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base C (30/26);  new base T (3/3);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACCCCAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCT  >  NC_002947/4050531‑4050818
                                                                                                                                                |                                                                                                                                               
ttACCCCAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCg                                                                                                                                             <  1:73525/149‑1 (MQ=255)
     caaaCGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGAGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCAGGGGCCCAAGCACACCACCGGCACCGTGGTa                                                                                                                                        >  6:249668/3‑149 (MQ=255)
            ccACCTCGGCGTGCGCGTGAAGTACGACCTGCCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCgg                                                                                                                                    >  6:27337/1‑146 (MQ=255)
              aCCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGaa                                                                                                                               >  3:187843/1‑149 (MQ=255)
               ccTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGTACCACGGTGCCGGtggtgt                                                                                                                              <  7:105618/149‑6 (MQ=255)
                   ggCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTgg                                                                                                                           <  5:315903/148‑1 (MQ=255)
                    gCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGta                                                                                                                         >  4:141048/1‑149 (MQ=255)
                                aGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAg                                                                                                             <  3:188443/149‑1 (MQ=255)
                                aGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAg                                                                                                             <  2:66836/149‑1 (MQ=255)
                                 gTACGACCTGCCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAgg                                                                                                            <  5:27337/149‑1 (MQ=255)
                                   aCGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAggg                                                                                                           <  3:245641/148‑1 (MQ=255)
                                          gTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAg                                                                                                                   <  8:81664/133‑1 (MQ=255)
                                          gTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAg                                                                                                                   >  7:81664/1‑133 (MQ=255)
                                                 gcctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGc                                                                                              >  1:197271/1‑147 (MQ=255)
                                                  cctgGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGa                                                                                            <  3:337095/148‑1 (MQ=255)
                                                              gcgTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGt                                                                               >  2:171773/1‑149 (MQ=255)
                                                                gTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTg                                                                                 <  2:186698/145‑1 (MQ=255)
                                                                gTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTg                                                                                 >  1:186698/1‑145 (MQ=255)
                                                                    cggcgTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtactac                                                                         >  2:188795/1‑149 (MQ=255)
                                                                     ggcgTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtactac                                                                         >  2:18242/1‑148 (MQ=255)
                                                                         ttCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGa                                                                    >  8:104263/1‑149 (MQ=255)
                                                                           cAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGa                                                                    >  4:286920/1‑147 (MQ=255)
                                                                           cAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGa                                                                    >  8:9514/1‑147 (MQ=255)
                                                                           cAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCACGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGa                                                                    >  1:225390/1‑147 (MQ=255)
                                                                            aaCGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtactactac                                                                      >  4:131526/1‑144 (MQ=255)
                                                                            aaCGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtactactac                                                                      <  3:131526/144‑1 (MQ=255)
                                                                              cGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGct                                                               <  3:141048/149‑1 (MQ=255)
                                                                              cGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGct                                                               <  2:197271/149‑1 (MQ=255)
                                                                              cGTCCACCCCGAAACCAACGGCAACTCCACCCGCCCCTGCCACCTGCGGCCCAAGCACACCACCGGCACCGAGCTACCCCTAAAACACGTAAACAAGCAGCAGGGTGAACTGCCAGGCCAGTACAAGCATGCTTACAACAACCACAGct                                                               >  8:244229/1‑149 (MQ=255)
                                                                               gTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGctc                                                              >  7:223836/1‑149 (MQ=255)
                                                                               gTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGctc                                                              >  4:313892/1‑149 (MQ=255)
                                                                                   aCCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGAt                                                          <  8:223836/149‑1 (MQ=255)
                                                                                      ccGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGAtgt                                                        >  3:54622/1‑148 (MQ=255)
                                                                                       cGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGAtgtg                                                       >  3:56450/1‑148 (MQ=255)
                                                                                         aaaGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGaaa                                                    <  1:18242/149‑1 (MQ=255)
                                                                                              aaCGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCAt                                               >  4:311228/1‑149 (MQ=255)
                                                                                              aaCGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCAt                                               >  7:241821/1‑149 (MQ=255)
                                                                                                  gCAACTCCAGCCGCGCCTGGCACATGGGGCCCAAGCACACCACCGGCACCCTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACAACTACGACAGCTCTGATGTGAAACGCATCgg                                            >  2:110929/1‑148 (MQ=255)
                                                                                                     aCTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGc                                        <  4:54622/149‑1 (MQ=255)
                                                                                                         cAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgatg                                    <  1:188795/149‑1 (MQ=255)
                                                                                                            ccGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCgatgat                                  >  2:8330/1‑148 (MQ=255)
                                                                                                            ccGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGatgatg                                 <  3:286920/149‑1 (MQ=255)
                                                                                                              gcgcCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGaa                               <  1:8330/149‑1 (MQ=255)
                                                                                                                          tGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGcc                     >  7:4332/1‑147 (MQ=255)
                                                                                                                            gggACCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGa                   <  7:244229/147‑1 (MQ=255)
                                                                                                                             ggCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGt                <  7:9514/149‑1 (MQ=255)
                                                                                                                              gCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGc                                           <  5:126335/121‑1 (MQ=255)
                                                                                                                              gCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGc                                           >  6:126335/1‑121 (MQ=255)
                                                                                                                               cccAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCTGGGTGAACTGCCAGGCGTCTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTgg              <  1:110929/149‑1 (MQ=255)
                                                                                                                                  aaGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtacta                                                                          >  7:181406/1‑86 (MQ=255)
                                                                                                                                  aaGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTtacta                                                                          <  8:181406/86‑1 (MQ=255)
                                                                                                                                    gCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCga                                      >  6:346800/1‑120 (MQ=255)
                                                                                                                                    gCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCga                                      <  5:346800/120‑1 (MQ=255)
                                                                                                                                        tctaccGGTACCACGGTGCCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGcc            >  3:82928/4‑142 (MQ=255)
                                                                                                                                        tctaccGGTACCACGGTGCCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGcc            <  4:82928/139‑1 (MQ=255)
                                                                                                                                         ccaccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACctg    <  8:4332/149‑1 (MQ=255)
                                                                                                                                          taccGGTACCACGGTGCCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACctga   >  7:266832/2‑148 (MQ=255)
                                                                                                                                          taccGGTACCACGGTGCAGTTAGAACTGGTATACATGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGTCCGAGGTGGCCATTAcat     <  8:266832/146‑3 (MQ=255)
                                                                                                                                          caccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACctgc   >  2:26288/1‑149 (MQ=255)
                                                                                                                                          caccGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGATGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACctgc   <  4:187843/149‑1 (MQ=255)
                                                                                                                                          caccGGCACCGTAGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACctgc   >  5:230895/1‑149 (MQ=255)
                                                                                                                                           accGGTACCACGGTGCCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACctgct  >  4:65769/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TTACCCCAACGCCCACCTCGGCGTGCGCGTGAAGTACGACCTGTCACCTGCCTGGCAGTTGCGCGTGGCGGCGTTCAACGTCGACCCCGAAAGCAACGGCAACTCCAGCCGCGCCTGGCACCTGGGGCCCAAGCACACCACCGGCACCGTGGTACCGGTAGAACTGGTATACAAGCTGCAGGGTGAACTGCCAGGCGAGTACAAGCTTGGTTACTACTACGACAGCTCTGATGTGAAACGCATCGGCAGCGATGATGAAGTGTCCGGCCGAGGTGGCCATTACCTGCT  >  NC_002947/4050531‑4050818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑