Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 353,067 A→G 100% intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947353,0670AG100.0% 150.4 / NA 46intergenic (+34/+84)hisF/cbcVimidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/23);  total (23/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCACC  >  NC_002947/352932‑353211
                                                                                                                                       |                                                                                                                                                  
cATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                                       >  5:252632/1‑149 (MQ=255)
 aTGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTcc                                                                                                                                      <  1:344427/149‑1 (MQ=255)
   gCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCca                                                                                                                                    >  8:323498/1‑149 (MQ=255)
    ccAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCca                                                                                                                                    >  3:145887/1‑148 (MQ=255)
      aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAg                                                                                                                                 <  7:249838/149‑1 (MQ=255)
          cgGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGttt                                                                                                                             <  4:145887/149‑1 (MQ=255)
           ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTg                                                                                                                            <  5:130671/149‑1 (MQ=255)
            gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGc                                                                                                                           <  8:89637/149‑1 (MQ=255)
                 tgcggaCAGCATCTTCCACTTTGTCGAGGACACAGTGCCGGAGGCCAAGGACTACATGGTTTAGCTCTTGATCTTCTTTCTCTGAGACATTGACCTGGTCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTgag                                                                                                                       <  2:77206/147‑1 (MQ=21)
                  gcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTgag                                                                                                                       <  7:323498/147‑1 (MQ=255)
                    ggcCAGCATCTTCCACTTTGGCGAGTACACAGTTCCGGAGGCCAATGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑Atct                                                                                                                  <  7:44353/149‑1 (MQ=255)
                       cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCGCTACCACACGGTTTCTGAG‑Attagtt                                                                                                               >  5:37654/1‑144 (MQ=38)
                          cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTgg                                                                                                            <  5:231081/149‑1 (MQ=255)
                            tCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTgg                                                                                                            >  2:211205/1‑147 (MQ=255)
                              ttCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGc                                                                                                        >  5:68602/1‑149 (MQ=255)
                              ttCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCGCACCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGc                                                                                                        >  1:134764/1‑149 (MQ=255)
                               tCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCGCACCCAAAGGTTTGCTGAG‑ATATGTTTGGGAGCg                                                                                                       >  6:96041/1‑149 (MQ=39)
                                ccACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGa                                                                                                          <  6:252632/145‑1 (MQ=255)
                                 cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                                          >  1:343506/1‑129 (MQ=255)
                                   cTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCACGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCg                                                                                                   >  1:250195/1‑149 (MQ=255)
                                        gCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑cc                                                                                              <  6:141578/148‑1 (MQ=255)
                                                  aGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTGTGTGGGAGCGGGCGTG‑CCCGCGAAGGGcc                                                                                   >  1:251558/1‑149 (MQ=255)
                                                    tGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCcgc                                                                                 <  6:68602/149‑1 (MQ=255)
                                                      ccGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCcgcg                                                                                >  6:303112/1‑148 (MQ=255)
                                                         gAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCACCCCCAGGTTTGCAGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCCCGAAGc                                                                            >  8:29402/1‑149 (MQ=39)
                                                              cAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCcac                                                                                                                                   <  3:317677/91‑1 (MQ=255)
                                                              cAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCcac                                                                                                                                   >  4:317677/1‑91 (MQ=255)
                                                                       cATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGCCCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑CTCTCTGTGGGAGCGGGCGGG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCt                                                               <  7:29402/148‑1 (MQ=255)
                                                                       cATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTATGTGGGAGCGGGCGTG‑CCCCCGAAGGGCCGCCAAGCGGCCCCAATTGCt                                                               >  6:95883/1‑148 (MQ=255)
                                                                        aTGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTGCCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCtt                                                              <  1:211205/149‑1 (MQ=255)
                                                                                   cgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTc                                                  <  5:303112/149‑1 (MQ=255)
                                                                                           tcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGag                                          <  2:251558/149‑1 (MQ=255)
                                                                                             gtTGGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGGGGGGACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagca                                        <  5:96041/149‑1 (MQ=255)
                                                                                             gtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagca                                        <  7:101970/149‑1 (MQ=255)
                                                                                                   tGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCgg                                                                                                      <  6:171821/82‑1 (MQ=255)
                                                                                                   tGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCgg                                                                                                      >  5:171821/1‑82 (MQ=255)
                                                                                                   tGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGCAGGGCCGCGAAGCGGCCCCCATTGCTTGAACCGTGGTTGTTGCCGAGCAGcgcgg                                  >  5:16467/1‑148 (MQ=38)
                                                                                                     aTCCTTTGACCGGGGCTGATTTGCCGCCGTGCGCGGCCACGCCCGCTCCCACCGGTTGGCTGAG‑ATGTGTGGGGTCGGGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTg                                 <  6:37654/148‑1 (MQ=25)
                                                                                                       ccATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGt                               >  1:259908/1‑148 (MQ=255)
                                                                                                          ttGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAg                           >  7:226782/1‑149 (MQ=255)
                                                                                                              ccGGGGCTGGTTTGCAGCCCTTCGCGGGCACGCCCGCTCCGACAGGTTTGCTGAG‑ATCTCTGTGGTAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCg                       <  5:95883/149‑1 (MQ=255)
                                                                                                                     tgctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATccc                >  6:333312/1‑149 (MQ=255)
                                                                                                                           gCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGCAGCGGCCCCAATTGCTTCAACCGGGGGTGTTGCCGCGCAGCGCGGGGTAAAGCGCGGGATCCCCGAGTa          >  7:292697/1‑149 (MQ=37)
                                                                                                                                ccTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATcccc     <  2:250195/149‑1 (MQ=255)
                                                                                                                                 cTTCGCGGGCACGCCCGCTCCCACAGAGAT‑CTCAGCAAACCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCcca    >  8:59511/1‑149 (MQ=18)
                                                                                                                                   tCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCccacc  >  8:316363/1‑149 (MQ=255)
                                                                                                                                    cgcgGGCACGCCCGCTCCCACAGGTTGGCTGAG‑ATCTCTGGGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCccac   <  1:336053/147‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                                  
CATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAG‑ATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCACC  >  NC_002947/352932‑353211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑