Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,080,919	A→T	7.3%	intergenic (‑143/+20)	amaC ← / ← dpkA	D‑lysine aminotransferase/delta 1‑piperideine‑2‑carboxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,080,919	0	A	T	7.3%	190.8 / 3.2	65	intergenic (‑143/+20)	amaC/dpkA	D‑lysine aminotransferase/delta 1‑piperideine‑2‑carboxylate reductase
Reads supporting (aligned to +/- strand):  ref base A (27/33);  new base T (2/3);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACAGCGTGCAGCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATGCGCGTCAGCCCCACCGCCTGCATG  >  NC_002947/4080773‑4081065                                                                                                                                                     |                                                                                                                                                   acacAGCGTGCAGCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTaaa                                                                                                                                                  >  7:21208/1‑149 (MQ=255)   acaGCGTGCAGCTCCTTGGATCAGGACGGTACGGAAAGTAAGTGTTGAGCATACCCGGAGTGCGGGTTAAGGAACATCCACTATTGTGAAAGGTTGTATATGCAAAATTGCATGACCGGGA‑TGAGGGCTGGGATTTGCGCG‑GGTAAccc                                                                                                                                                >  8:214999/1‑149 (MQ=255)            aGCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAggg                                                                                                                                       <  5:155096/149‑1 (MQ=255)            aGCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTCTTCGCG‑GGTTTACCCGCGaaaaa                                                                                                                                       <  5:120849/149‑4 (MQ=255)            aGCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGGCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAggg                                                                                                                                       <  5:317679/149‑1 (MQ=255)            aGCGCCTTGGATCAGGACGTTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTTCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑TGTAAACCCGCGAAggg                                                                                                                                       <  5:83376/149‑1 (MQ=255)             gCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGACTGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAggg                                                                                                                                       <  1:235368/149‑1 (MQ=255)             gCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAgggg                                                                                                                                      >  7:319122/1‑149 (MQ=255)              cTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGc                                                                                                                                     <  3:141167/149‑1 (MQ=255)                 cTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGcc                                                                                                                                    <  8:21208/147‑1 (MQ=255)                     gATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCa                                                                                                                              <  4:19896/149‑1 (MQ=255)                         aGGACGGTACCGAAACTCAGTGTGGACCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGTTTTTCTATGCAACATTGCATGACCGGTA‑TGAGGGCTGGCTTTTTCGCG‑GGTTTACCCGCGAAGGGGCCAAATCAGc                                                                                                                            <  7:214999/147‑1 (MQ=255)                            aCGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAagc                                                                                                                       <  1:172084/149‑1 (MQ=255)                              ggTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTTTACCCGCGaa                                                                                                                                          >  3:195699/1‑128 (MQ=255)                              ggTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTTTACCCGCGaa                                                                                                                                          <  4:195699/128‑1 (MQ=255)                                     aaaGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTc                                                                                                              <  8:319122/149‑1 (MQ=255)                                         tCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGc                                                                                                          >  4:72327/1‑149 (MQ=255)                                           cgtgtCGAGCCTTCCCGGAGTGCGGGTTCAGGCACATCCACTCTTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGc                                                                                                          <  4:260383/146‑1 (MQ=255)                                           aGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCt                                                                                                        >  1:77592/1‑149 (MQ=255)                                             tgtTGAGCACACCCGGAGGGAGGGTTCAGGAACATCCACTATTGTGCAAGGTTGACTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGGAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCttt                                                                                                      <  5:336531/149‑1 (MQ=255)                                              gtTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCttt                                                                                                      >  4:127744/1‑148 (MQ=255)                                              gtTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTc                                                                                                     <  4:142992/149‑1 (MQ=255)                                                  atcATACCCGGAGTGCGGGTTCATGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAAc                                                                                                  <  2:271969/146‑1 (MQ=255)                                                      tACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCaaa                                                                                                                                 >  7:161522/1‑113 (MQ=255)                                                      tACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCaaa                                                                                                                                 <  8:161522/113‑1 (MQ=255)                                                         ccGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTc                                                                                          >  1:319570/1‑149 (MQ=255)                                                          cGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCa                                                                                                                              <  6:95211/112‑1 (MQ=255)                                                          cGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCa                                                                                                                              >  5:95211/1‑112 (MQ=255)                                                                 cGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCg                                                                                  >  7:291799/1‑149 (MQ=255)                                                                  gggTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCGCGGTAAACCCGCGAAGGGGCCAAATCAGCCaa                                                                                                                         <  1:211415/110‑1 (MQ=255)                                                                  gggTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCaa                                                                                                                         >  2:211415/1‑109 (MQ=255)                                                                     ttCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCAc                                                                              >  1:237731/1‑149 (MQ=255)                                                                       cAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCAcc                                                                             <  3:127744/148‑1 (MQ=255)                                                                          gAACATCCACTATTGTGCAAGCTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCATTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCcct                                                                         >  4:159465/1‑149 (MQ=255)                                                                               tCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCa                                                                                                             <  8:2977/108‑1 (MQ=255)                                                                               tCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCAGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCa                                                                                                             >  7:2977/1‑108 (MQ=255)                                                                                 cACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCcctcctcct                                                                   <  2:77592/148‑1 (MQ=255)                                                                                  aCTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCg                                                                 <  2:115419/149‑1 (MQ=255)                                                                                  aCTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCg                                                                 <  3:72327/149‑1 (MQ=255)                                                                                  aCTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCg                                                                 >  3:324831/1‑149 (MQ=255)                                                                                   cTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCgg                                                                >  7:25859/1‑149 (MQ=255)                                                                                        gtgCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCAc                                                            >  4:170375/1‑148 (MQ=255)                                                                                                  gTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTc                                                                                                              <  8:149884/88‑1 (MQ=255)                                                                                                  gTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTc                                                                                                              >  7:149884/1‑88 (MQ=255)                                                                                                        gCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCTACTCCTGCTCGGTCACCg                                                                                  <  6:169521/110‑1 (MQ=255)                                                                                                        gCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCg                                                                                  >  5:169521/1‑110 (MQ=255)                                                                                                          aaCATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTc                                                         <  6:351388/133‑1 (MQ=255)                                                                                                          aaCATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTc                                                         >  5:351388/1‑133 (MQ=255)                                                                                                             aTTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGTGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGcgccgc                                      <  4:324831/149‑1 (MQ=255)                                                                                                                 cATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGc                                  <  7:275274/149‑1 (MQ=255)                                                                                                                 cATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTACTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGc                                  >  8:110788/1‑149 (MQ=255)                                                                                                                  aTGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCg                                                                 <  5:270086/117‑1 (MQ=255)                                                                                                                  aTGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCg                                                                 >  6:270086/1‑117 (MQ=255)                                                                                                                  aTGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGcc                                 >  8:143369/1‑149 (MQ=255)                                                                                                                      ccGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGc                             <  8:25859/149‑1 (MQ=255)                                                                                                                      ccGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGAAGCTCTTACAGGCCTATCAACTCCTGCTCGGTAACCGCCACACCCTCCACCTCGGCCACCTCGCGCTCACGGAAGCGCCGAACGCCCgg                              >  1:64856/1‑148 (MQ=255)                                                                                                                              gAGGGCTGGCTTTTTCGCT‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCGCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATgcgc                       <  8:291799/148‑1 (MQ=255)                                                                                                                                gggCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCAcccc                                                                           <  4:32074/94‑1 (MQ=255)                                                                                                                                gggCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCAcccc                                                                           >  3:32074/1‑94 (MQ=255)                                                                                                                                gggCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATGCGCGTc                    <  2:319570/149‑1 (MQ=255)                                                                                                                                           ttCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTc                                                                                                <  8:156194/62‑1 (MQ=255)                                                                                                                                           ttCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTc                                                                                                >  7:156194/1‑62 (MQ=255)                                                                                                                                              gcg‑GGTTTACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATGCGCGTCAGCCCCACCGCCTg      >  3:92970/1‑149 (MQ=255)                                                                                                                                                g‑ggTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATGCGCGTCAGCCCCACCGCCTGCa    >  7:73586/1‑149 (MQ=255)                                                                                                                                                g‑ggTAAACCCGCGAAGGGGCCAAAACAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCATGATCGGTCACCGCCACCCCCTCCTCCTCGGCAACCACGCGCTCACGGTACCGCCCCTCGCCCGGAATGCGCGAAACCCACACAGCCtcc     >  7:48177/1‑146 (MQ=255)                                                                                                                                                   gTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATGCGCGTCAGCCCCACCGCCTGCAtg  <  3:170375/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACACAGCGTGCAGCTCCTTGGATCAGGACGGTACGGAAAGTCAGTGTTGAGCATACCCGGAGTGCGGGTTCAGGAACATCCACTATTGTGCAAGGTTGTCTATGCAACATTGCATGACCGGGA‑TGAGGGCTGGCTTTTTCGCG‑GGTAAACCCGCGAAGGGGCCAAATCAGCCAAGCAGCTCTTTCAGGCCTTTCAACTCCTGCTCGGTCACCGCCACCCCCTCCTCCTCGGCCACCTCGCGCTCACGGTAGCGCCGCTCGCCCGGCATGCGCGTCAGCCCCACCGCCTGCATG  >  NC_002947/4080773‑4081065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑