Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,082,777 A→T 5.4% N241I (AAT→ATT)  PP_3592 → RpiR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,082,7770AT 5.4% 230.6 / 2.2 74N241I (AAT→ATT) PP_3592RpiR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (36/34);  new base T (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGTTGAAAAACGCTTGGAAGCAACTGCCG  >  NC_002947/4082629‑4082923
                                                                                                                                                     |                                                                                                                                                    
ctgctgGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCaa                                                                                                                                                      <  7:10403/149‑1 (MQ=255)
 tgctgGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAAt                                                                                                                                                     >  8:293339/1‑149 (MQ=255)
    tgGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGcc                                                                                                                                                  <  2:73816/149‑1 (MQ=255)
       gCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCcgac                                                                                                                                               >  6:36805/1‑149 (MQ=255)
        ccGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCcgac                                                                                                                                               >  3:145492/1‑148 (MQ=255)
                aaGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTc                                                                                                                                      <  4:118656/149‑1 (MQ=255)
                 aGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTcc                                                                                                                                     <  4:149339/149‑1 (MQ=255)
                  gACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTc                                                                                                                                      >  6:312364/1‑147 (MQ=255)
                  gACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTc                                                                                                                                      <  5:312364/147‑1 (MQ=255)
                     aGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTc                                                                                                                                      >  6:38419/1‑144 (MQ=255)
                     aGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTc                                                                                                                                      <  5:38419/144‑1 (MQ=255)
                     aGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCAt                                                                                                                                 <  4:145492/149‑1 (MQ=255)
                       cgcCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATcc                                                                                                                               >  4:280537/1‑149 (MQ=255)
                          ccTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCa                                                                                                                            >  6:181052/1‑149 (MQ=255)
                            tggtggTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCAcc                                                                                                                          >  4:244718/1‑149 (MQ=255)
                              gtggtgTTCGAGGCGCGCCGTTATTCCCGCAATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GATGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGa                                                                                                                        <  3:41979/149‑1 (MQ=255)
                                ggtgTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGt                                                                                                                      >  5:250700/1‑149 (MQ=255)
                                 gtgtTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGt                                                                                                                      <  3:31806/148‑1 (MQ=255)
                                   gtTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATc                                                                                                                                >  7:193211/1‑136 (MQ=255)
                                   gtTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATc                                                                                                                                <  8:193211/136‑1 (MQ=255)
                                      cGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCGAc                                                                                                                 >  1:90174/1‑149 (MQ=255)
                                      cGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAAc                                                                                                                 <  3:168351/148‑1 (MQ=255)
                                            gcgcCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCt                                                                                                          <  3:280537/149‑1 (MQ=255)
                                            gcgcCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCt                                                                                                          >  6:222714/1‑149 (MQ=255)
                                             cgcCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTg                                                                                                         <  7:293339/149‑1 (MQ=255)
                                              gcCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTgg                                                                                                        <  1:133310/149‑1 (MQ=255)
                                                  ttATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTgg                                                                                                        <  8:176971/145‑1 (MQ=255)
                                                  ttATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTgg                                                                                                        >  7:176971/1‑145 (MQ=255)
                                                     ttCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCa                                                                                                 <  6:250700/149‑1 (MQ=255)
                                                      tCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCAc                                                                                                >  3:211554/1‑149 (MQ=255)
                                                        ccGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCt                                                                                              >  2:267296/1‑149 (MQ=255)
                                                           ccATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTcg‑a                                                                                          <  3:244718/149‑1 (MQ=255)
                                                             aTGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTcg‑acg                                                                                        <  5:222714/149‑1 (MQ=255)
                                                                           tgCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCATTGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTgg                                                                            >  6:161724/1‑147 (MQ=255)
                                                                           tgCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCATTGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTgg                                                                            <  5:156725/147‑1 (MQ=255)
                                                                           tgCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCATTGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTgg                                                                            <  5:161724/147‑1 (MQ=255)
                                                                           tgCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCATTGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTgg                                                                            >  6:156725/1‑147 (MQ=255)
                                                                           tgCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTgg                                                                            <  4:211554/147‑1 (MQ=255)
                                                                             ccAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTgg                                                                                                        >  1:149238/1‑118 (MQ=255)
                                                                             ccAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTgg                                                                                                        <  2:149238/118‑1 (MQ=255)
                                                                                   ggCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGa                                                                  <  5:181052/149‑1 (MQ=255)
                                                                                    gcgcgc‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAt                                                                 <  6:342690/149‑1 (MQ=255)
                                                                                       cgc‑gAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑gg                                                              >  6:107578/1‑148 (MQ=255)
                                                                                                tGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAgg                                                     >  6:333784/1‑149 (MQ=255)
                                                                                                tGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAgg                                                     >  2:41333/1‑149 (MQ=255)
                                                                                                  gCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAAGCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTc                                                   >  7:236164/1‑149 (MQ=255)
                                                                                                  gCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTc                                                   <  4:71265/149‑1 (MQ=255)
                                                                                                         ggTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGa                                                       >  1:137887/1‑138 (MQ=255)
                                                                                                          gTCACCCTGGTGACCGACACCGTCTGTGACGGGGCGGATGCCAATGCAGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTTTTCTGGGAATCCACCTAG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GTTCAACGAGGTCTGCaagaa                                           <  4:319059/149‑1 (MQ=255)
                                                                                                             aCCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTc                                                                                             >  5:226968/1‑98 (MQ=255)
                                                                                                             aCCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTc                                                                                             <  6:226968/98‑1 (MQ=255)
                                                                                                               ccTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCg                                      <  5:333784/149‑1 (MQ=255)
                                                                                                               ccTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCg                                      >  4:337570/1‑149 (MQ=255)
                                                                                                                cTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCg                                      >  8:202729/1‑148 (MQ=255)
                                                                                                                   gTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGTTCGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGAc                                   <  1:267296/149‑1 (MQ=255)
                                                                                                                    tGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCg                                 <  5:107578/149‑1 (MQ=255)
                                                                                                                       ccGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGATCGGTCAACGAGGTCTGCAAGAAGCTCGGACCGGa                               <  1:41333/149‑1 (MQ=255)
                                                                                                                        cGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGCTGCTGTCGTCGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGAGCGGATg                             >  6:75390/1‑149 (MQ=255)
                                                                                                                        cGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGAcc                                  >  5:170554/1‑144 (MQ=255)
                                                                                                                        cGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGAcc                                  <  6:170554/144‑1 (MQ=255)
                                                                                                                          acacCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCAt                                                                      <  1:114604/107‑1 (MQ=255)
                                                                                                                          acacCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCAt                                                                      >  2:114604/1‑107 (MQ=255)
                                                                                                                              cTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGTTGaa                        >  7:266347/1‑148 (MQ=255)
                                                                                                                              cTTCTGTGACTGGGCGGACACCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCaagaag                                          <  7:66103/130‑1 (MQ=255)
                                                                                                                              cTTCTGTGACTGGGCGGACACCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCaagaag                                          >  8:66103/1‑130 (MQ=255)
                                                                                                                                 cTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGc                                                <  4:135899/121‑1 (MQ=255)
                                                                                                                                    tgACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGTTGAAAAACg                   <  5:36805/147‑1 (MQ=255)
                                                                                                                                      aCTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGTTGAAAAACGCTTg               >  7:267456/1‑149 (MQ=255)
                                                                                                                                                  ccAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGTTGAAAAACGCTTGGAAGCAACTGcc   >  3:174225/1‑149 (MQ=255)
                                                                                                                                                   cAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGtt                           >  7:147087/1‑124 (MQ=255)
                                                                                                                                                   cAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGtt                           <  8:147087/124‑1 (MQ=255)
                                                                                                                                                   cAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGTTGAAAAACGCTTGGAAGCAACTGCCg  >  4:30836/1‑149 (MQ=255)
                                                                                                                                                   cAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGTTGAAAAACGCTTGGAAGCAACTGCCg  >  4:201607/1‑149 (MQ=255)
                                                                                                                                                   cAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGTTGAAAAACGCTTGGAAGCAACTGCCg  >  3:160915/1‑149 (MQ=255)
                                                                                                                                                   cAATGCCGACGAGGTGTTCAGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGTTGAAAAAAGCTTGGAAGCAACTGCCg  >  4:328010/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGC‑GAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCG‑ACGATGCTGTCGTGGGTGCATCTGAT‑GGTCAACGAGGTCTGCAAGAAGCTCGGACCGGATGTTGAAAAACGCTTGGAAGCAACTGCCG  >  NC_002947/4082629‑4082923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑