Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,098,301 A→G 8.1% L130P (CTG→CCG)  qor‑II ← quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,098,3010AG 8.1% 137.6 / 2.3 49L130P (CTG→CCG) qor‑IIquinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (25/20);  new base G (2/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTCCACACTTTGCAGGCGCGATACCACCCCTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGC  >  NC_002947/4098156‑4098448
                                                                                                                                                 |                                                                                                                                                   
cTCCACACTTTGCAGGCGCGATACCACCCCTATCACCGTCGCCCCCAGGTGTCGCGCCCTAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTAGAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGat                                                                                                                                                  <  1:310306/149‑1 (MQ=255)
 tCCACACTTTGCAGGCGCGATTCGACCCCTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCAACAGCTGGCCCAAAGCACCGGCCGAACCCTACAACATCCCCAGGGTGCCCGGCCCTACGGCATAGGTGGTATTCAACAggtg                                                                                                                                                 >  2:234115/1‑146 (MQ=255)
   gactcTTTGCATGCGAGATACCAGCCCTATGACCGTCGCCCCCAGGTGTCGCGGCCAAGGCAGCATGATGTTGGAGATAGCACCTGCCGCACCGTACATCAAGAGCTTGGTGCCCGGCCGTAGGGCATAGGTGGTTTTCAACa                                                                                                                                                     <  4:231868/139‑1 (MQ=255)
   cacaCTTTGCAGGCGCGATACCACCCCTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGGACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCATCAGATAtt                                                                                                                                               <  2:273447/149‑1 (MQ=255)
   cacaCTTTGCAGGCGCGATACCACCCCTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACACCACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACa                                                                                                                                                     >  3:231868/1‑143 (MQ=255)
     cacTTTGCAGGCGCGATACCACCCCTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTgc                                                                                                                                             <  4:128509/149‑1 (MQ=255)
                  cgATACCACCCCTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCt                                                                                                                                <  8:192798/149‑1 (MQ=255)
                   gATACCACCCCTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCtt                                                                                                                               <  3:126799/149‑1 (MQ=255)
                       cctccccTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGTACCATCAGCTGGCCCAAAGCAGCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTGCGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGaa                                                                                                                            <  1:250499/145‑1 (MQ=255)
                        caccCCTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTc                                                                                                                                   >  6:150366/1‑140 (MQ=255)
                        caccCCTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTc                                                                                                                                   <  5:150366/140‑1 (MQ=255)
                                tCACCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCt                                                                                                                  >  1:19648/1‑149 (MQ=255)
                                  aCCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCATACAACAACACCTGGGTGCCCGTCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCCTGATTCCCTTGCACAGCACCGCTgc                                                                                                                >  1:270749/1‑149 (MQ=255)
                                   ccGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACGGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTgcg                                                                                                               >  8:298786/1‑149 (MQ=255)
                                                gtgtCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGaa                                                                                                                            >  6:284893/1‑123 (MQ=255)
                                                gtgtCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGaa                                                                                                                            <  5:284893/123‑1 (MQ=255)
                                                 tgtCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGtcctc                                                                                                 <  3:15156/149‑1 (MQ=255)
                                                   tCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGtcctcc                                                                                                >  3:328641/1‑148 (MQ=255)
                                                     gcgcCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAAc                                                                                             >  8:211735/1‑149 (MQ=255)
                                                     gcgcCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAAc                                                                                             >  6:302885/1‑149 (MQ=255)
                                                     gcgcCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAAc                                                                                             >  2:144060/1‑149 (MQ=255)
                                                       gcCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACGGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTg                                                                                           >  6:98917/1‑149 (MQ=255)
                                                             ggCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGcggcgg                                                                                     <  2:201521/149‑1 (MQ=255)
                                                                aCCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAg                                                                                  >  4:49341/1‑149 (MQ=255)
                                                                 ccATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACGGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGc                                                                                 <  5:98917/149‑1 (MQ=255)
                                                                       gCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAAc                                                                            >  6:168837/1‑148 (MQ=255)
                                                                           gCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAg                                                                         <  3:49341/147‑1 (MQ=255)
                                                                               aaaGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTc                                                                   <  2:19648/149‑1 (MQ=255)
                                                                                    aCCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTTGGCCg                                                              >  2:194697/1‑149 (MQ=255)
                                                                                        gCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGaa                                                          >  5:17767/1‑149 (MQ=255)
                                                                                            cACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGc                                                      >  7:300753/1‑149 (MQ=255)
                                                                                               cGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCgg                                                   <  6:17767/149‑1 (MQ=255)
                                                                                                gTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGc                                                  >  2:116696/1‑149 (MQ=255)
                                                                                                    aacaacaCCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACt                                              <  4:328641/149‑1 (MQ=255)
                                                                                                       aacaCCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACGGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGc                                           <  7:298786/149‑1 (MQ=255)
                                                                                                       aacaCCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTgg                                            >  6:307577/1‑148 (MQ=255)
                                                                                                           ccTGGGTGCCCGGCCATACGGCATAGGTGCTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAg                                       <  2:270749/149‑1 (MQ=255)
                                                                                                                  gCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCg                                                    >  4:220217/1‑129 (MQ=255)
                                                                                                                  gCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCg                                                    <  3:220217/129‑1 (MQ=255)
                                                                                                                   cccGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCaccga                                <  1:116696/148‑1 (MQ=255)
                                                                                                                     cGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAgg                                      >  5:123618/1‑140 (MQ=255)
                                                                                                                     cGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAgg                                      <  6:123618/140‑1 (MQ=255)
                                                                                                                         ccTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGccc                         <  7:211735/149‑1 (MQ=255)
                                                                                                                           tACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCgggtcgc                       >  2:156440/1‑144 (MQ=255)
                                                                                                                                cATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATcgcg                  >  2:321571/1‑149 (MQ=255)
                                                                                                                                    ggtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAg              <  5:168837/149‑1 (MQ=255)
                                                                                                                                      tggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGc             >  7:99942/1‑148 (MQ=255)
                                                                                                                                      tggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCg            >  2:88538/1‑149 (MQ=255)
                                                                                                                                         ttttCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACAAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGAcc         >  5:87752/1‑149 (MQ=255)
                                                                                                                                          tttCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGAccc        >  6:149010/1‑149 (MQ=255)
                                                                                                                                                cAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGc  >  1:198555/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CTCCACACTTTGCAGGCGCGATACCACCCCTATCACCGTCGCCCCCAGGTGTCGCGCCCAAGGCACCATCAGCTGGCCCAAAGCACCGGCCGCACCGTACAACAACACCTGGGTGCCCGGCCCTACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGC  >  NC_002947/4098156‑4098448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑