Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,133,979 C→G 10.3% A351P (GCA→CCA) ‡ PP_3638 ← acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,133,9790CG10.3% 171.0 / 8.6 58A351P (GCA→CCA) ‡PP_3638acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (29/23);  new base G (3/3);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

ACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGT  >  NC_002947/4133832‑4134121
                                                                                                                                                   |                                                                                                                                              
acacCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACgcctgcc                                                                                                                                               <  4:321498/149‑1 (MQ=255)
     acTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCg                                                                                                                                          >  1:272914/1‑149 (MQ=255)
         gggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTg                                                                                                                                       <  2:165908/148‑1 (MQ=255)
         gggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGtt                                                                                                                                        <  2:272914/147‑1 (MQ=255)
         gggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTg                                                                                                                                       <  2:171630/148‑1 (MQ=255)
          ggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCCCCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTg                                                                                                                                       >  2:304409/1‑147 (MQ=255)
               aGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCg                                                                                                                                <  6:95398/149‑1 (MQ=255)
                        aCGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAAt                                                                                                                       >  5:134497/1‑149 (MQ=255)
                          gACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTg                                                                                                                     >  8:11288/1‑149 (MQ=255)
                             cTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCAc                                                                                                                  <  5:226955/149‑1 (MQ=255)
                             cTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCAc                                                                                                                  >  3:147211/1‑149 (MQ=255)
                               cACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCAc                                                                                                                  >  2:301243/1‑147 (MQ=255)
                                   tGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCt                                                                                                            >  5:315001/1‑149 (MQ=255)
                                     gTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTg                                                                                                           <  5:21721/148‑1 (MQ=255)
                                     gTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTg                                                                                                           <  6:134497/148‑1 (MQ=255)
                                                  gTCGAACTGCGCCAGCACCTGCTGCCAACCTCCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCgg                                                                                             >  1:28817/1‑149 (MQ=255)
                                                     gAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGAGACGATCGGCAc                                                                                          >  6:200610/1‑149 (MQ=255)
                                                           cgcCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGc                                                                                    <  2:173671/149‑1 (MQ=255)
                                                             ccAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGcc                                                                                   >  4:338803/1‑148 (MQ=255)
                                                                              ccaccCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcgg                                                                 >  5:185857/1‑149 (MQ=255)
                                                                                accCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGc                                                               <  7:11288/149‑1 (MQ=255)
                                                                                accCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGc                                                               <  1:301243/149‑1 (MQ=255)
                                                                                 cccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCGATGGCgggag                                                              <  6:315001/149‑3 (MQ=255)
                                                                                   cTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                            <  8:90012/149‑1 (MQ=255)
                                                                                    tCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAAc                                                           >  7:73711/1‑149 (MQ=255)
                                                                                    tCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAAc                                                           >  1:341273/1‑149 (MQ=255)
                                                                                        gcgGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGACTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCtt                                                       >  8:221258/1‑149 (MQ=255)
                                                                                           ggTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGc                                                    <  8:73711/149‑1 (MQ=255)
                                                                                               gCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAgg                                                                                                              <  3:272517/87‑1 (MQ=255)
                                                                                               gCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAgg                                                                                                              >  4:272517/1‑87 (MQ=255)
                                                                                                 ccAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCACGCCATCGGCGGGCAAACACTTCGCCGTAtt                                              >  4:73706/1‑149 (MQ=255)
                                                                                                  cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGcctgcctg                                                                                                                                             >  7:137612/1‑53 (MQ=255)
                                                                                                  cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGcctgcctg                                                                                                                                             <  8:137612/53‑1 (MQ=255)
                                                                                                  cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTc                                             >  3:128463/1‑149 (MQ=255)
                                                                                                  cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTc                                             >  3:10584/1‑149 (MQ=255)
                                                                                                    aGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGc                                           <  6:185857/149‑1 (MQ=255)
                                                                                                     gCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCATTTGCACCAGACTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCa                                          <  7:221258/149‑1 (MQ=255)
                                                                                                          tGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCagag                                       >  4:211266/1‑147 (MQ=255)
                                                                                                          tGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCagag                                       <  3:211266/147‑1 (MQ=255)
                                                                                                             cACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAg                                  >  2:241387/1‑149 (MQ=255)
                                                                                                              aCGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGTACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGc                                 >  3:213159/1‑149 (MQ=255)
                                                                                                                   ccTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTc                                                      <  7:174965/123‑1 (MQ=255)
                                                                                                                   ccTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTc                                                      >  8:174965/1‑123 (MQ=255)
                                                                                                                    cTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgcc                           >  8:39239/1‑149 (MQ=255)
                                                                                                                       cAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTc                                                      <  8:117145/119‑1 (MQ=255)
                                                                                                                       cAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTc                                                      >  7:117145/1‑119 (MQ=255)
                                                                                                                          cAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGAGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTTCCGCCACt                     >  8:10144/1‑149 (MQ=255)
                                                                                                                             cacCGCGCTCATGCAACGCCTGCCTGGCGTTGCATTTAGGCGCTCAATTGCAACAGGCTGGCCGTGACGATAGTAAAGAAGGCCGACTCCCGCCATCTGCGGGCAAACCAATCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTgg                   >  6:126934/1‑148 (MQ=255)
                                                                                                                                cgcgCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTg               <  7:283174/149‑1 (MQ=255)
                                                                                                                                cgcgCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTg               >  8:283174/1‑149 (MQ=255)
                                                                                                                                   gcTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGCGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCaa            <  5:200610/149‑1 (MQ=255)
                                                                                                                                   ccgccagCAACGCCTGCCGGGCGGTGCAGTTCCGCGCGCACTTCCCCCACGCTGCCCGCGACGATCGGCACGAAGGCCGACCCCCGCCCCCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCaa            <  4:10584/143‑1 (MQ=255)
                                                                                                                                     tCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACt          >  3:161011/1‑149 (MQ=255)
                                                                                                                                     aactGCAACGCCAGGCAGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCaa            <  6:344698/144‑1 (MQ=255)
                                                                                                                                     aactGCAACGCCAGGCAGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCaa            >  5:344698/4‑147 (MQ=255)
                                                                                                                                        tGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGTCACTGGCCTGCAACTCCa       <  3:338803/149‑1 (MQ=255)
                                                                                                                                          cAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg      <  7:39239/148‑1 (MQ=255)
                                                                                                                                          cAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg      <  2:28817/148‑1 (MQ=255)
                                                                                                                                          cAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGt     >  1:335845/1‑149 (MQ=255)
                                                                                                                                          cAACGCCAGGCAGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGAAGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGt     >  7:230503/1‑149 (MQ=255)
                                                                                                                                           aaCGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt    >  1:128921/1‑149 (MQ=255)
                                                                                                                                              gcctgcctGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAATCCTTGCCGCCACTGGCCTGCAACTCCAGTTGt  >  7:229933/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
ACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGT  >  NC_002947/4133832‑4134121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑