Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,138,583 G→C 14.3% G28R (GGC→CGC) ‡ PP_3643 → oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,138,5830GC14.3% 88.1 / 6.9 35G28R (GGC→CGC) ‡PP_3643oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (19/11);  new base C (2/3);  total (21/14)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

GGTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGT  >  NC_002947/4138436‑4138721
                                                                                                                                                    |                                                                                                                                           
ggTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACgg                                                                                                                                            <  1:212563/149‑1 (MQ=255)
  tataCCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCt                                                                                                                                          <  6:203921/149‑1 (MQ=255)
               acaTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGT‑CGTCCt                                                                                                                            <  4:94627/149‑1 (MQ=255)
               acaTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCt                                                                                                                            <  5:54829/149‑1 (MQ=255)
                 aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGa                                                                                                                          >  6:349498/1‑149 (MQ=255)
                 aTCAACGATAAAAACCGCGGACCACCCCAGCCCTTCAGAGGTACTCCCGATGATCGAAGCAACTGTCTACAAGAACGTA‑CTGGGCTCCTTCCCCTCCGGCGAAACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGa                                                                                                                          >  5:74159/1‑149 (MQ=255)
                 aTCAACGATAAAAACCGCGGACCACAGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGa                                                                                                                          >  3:59035/1‑149 (MQ=255)
                    aaCGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGT‑CGTCCTgggtg                                                                                                                       <  3:261079/149‑5 (MQ=255)
                             aaCCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCt                                                                                                              >  7:77697/1‑149 (MQ=255)
                                                  ttCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGtcgt‑cgtc                                                                                                                              <  1:270819/112‑3 (MQ=255)
                                                  ttCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGtcgt‑cgtc                                                                                                                              >  2:270819/1‑110 (MQ=255)
                                                        ggTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCcgccgc                                                                                   <  8:77697/149‑1 (MQ=255)
                                                               ccGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                            >  5:213968/1‑149 (MQ=255)
                                                               ccGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                            >  8:234521/1‑149 (MQ=255)
                                                               ccGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                            >  7:247234/1‑149 (MQ=255)
                                                                 gatgatCGACGCAACTGTCTACAAGAACGTTGCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGCCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                           >  1:211579/1‑149 (MQ=255)
                                                                      tCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCCGGCCTGACCGCCAGCGCCTTCtcctcc                                                                                                      <  1:190134/117‑1 (MQ=255)
                                                                      tCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCtcctcc                                                                                                      >  2:190134/1‑116 (MQ=255)
                                                                             aaCTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAAc                                                               >  3:263843/1‑148 (MQ=255)
                                                                               gtGTCTACAAGCCGCTT‑CGGGGCGCCTCCCCGCCGGGCGTTACCGTCTTCCCCACCCCGCACCACGAGGGCTCGGTCGT‑GGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                            <  6:213968/148‑1 (MQ=25)
                                                                               cTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                            <  4:263843/149‑1 (MQ=255)
                                                                                     aCAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                      >  7:237189/1‑149 (MQ=255)
                                                                                       aaGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGa                                                    <  2:211579/149‑1 (MQ=255)
                                                                                           aCGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTc                                                 <  4:59035/148‑1 (MQ=255)
                                                                                                            cccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                            >  2:99885/1‑121 (MQ=255)
                                                                                                            cccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                            <  1:99885/121‑1 (MQ=255)
                                                                                                             ccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTAGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATc                                 >  5:274793/1‑147 (MQ=255)
                                                                                                               gTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAg                              >  3:319219/1‑148 (MQ=255)
                                                                                                                    gCGTTACCGTCATCACCCCCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGCCCGCCAGCGCCTTCTCCTCCCTGTCGAGGGCCCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAg                        >  6:101842/1‑149 (MQ=255)
                                                                                                                           cGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTg                 >  1:9127/1‑149 (MQ=255)
                                                                                                                                tcaCCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt            <  8:237189/149‑1 (MQ=255)
                                                                                                                                tcaCCACCCAGGACGACAACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt            >  5:271521/1‑149 (MQ=255)
                                                                                                                                 caccacCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt            >  8:242682/1‑148 (MQ=255)
                                                                                                                                   ccaccCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAc         <  8:247234/149‑1 (MQ=255)
                                                                                                                                    caccCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAcc        >  6:145532/1‑149 (MQ=255)
                                                                                                                                       tcAGGCCGACGACCGAGCCGTCGT‑CGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCTAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgc     >  1:302090/2‑149 (MQ=37)
                                                                                                                                          ggccgacgaccGAGCCGTCGT‑CGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGt  >  8:27803/4‑149 (MQ=37)
                                                                                                                                                    |                                                                                                                                           
GGTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGT  >  NC_002947/4138436‑4138721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑