Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,138,585	C→A	16.7%	G28G (GGC→GGA) ‡	PP_3643 →	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,138,585	0	C	A	16.7%	78.6 / 10.8	36	G28G (GGC→GGA) ‡	PP_3643	oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (19/11);  new base A (3/3);  total (22/14)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

TATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGT  >  NC_002947/4138438‑4138721                                                                                                                                                     |                                                                                                                                          tataCCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCt                                                                                                                                          <  6:203921/149‑1 (MQ=255)              acaTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGT‑CGTCCt                                                                                                                            <  4:94627/149‑1 (MQ=255)              acaTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCt                                                                                                                            <  5:54829/149‑1 (MQ=255)                aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGa                                                                                                                          >  6:349498/1‑149 (MQ=255)                aTCAACGATAAAAACCGCGGACCACCCCAGCCCTTCAGAGGTACTCCCGATGATCGAAGCAACTGTCTACAAGAACGTA‑CTGGGCTCCTTCCCCTCCGGCGAAACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGa                                                                                                                          >  5:74159/1‑149 (MQ=255)                aTCAACGATAAAAACCGCGGACCACAGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGa                                                                                                                          >  3:59035/1‑149 (MQ=255)                   aaCGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGT‑CGTCCTgggtg                                                                                                                       <  3:261079/149‑5 (MQ=255)                            aaCCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCt                                                                                                              >  7:77697/1‑149 (MQ=255)                                                 ttCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGtcgt‑cgtc                                                                                                                              <  1:270819/112‑3 (MQ=255)                                                 ttCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGtcgt‑cgtc                                                                                                                              >  2:270819/1‑110 (MQ=255)                                                       ggTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCcgccgc                                                                                   <  8:77697/149‑1 (MQ=255)                                                              ccGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                            >  8:234521/1‑149 (MQ=255)                                                              ccGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                            >  7:247234/1‑149 (MQ=255)                                                              ccGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                            >  5:213968/1‑149 (MQ=255)                                                                gatgatCGACGCAACTGTCTACAAGAACGTTGCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGCCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                           >  1:211579/1‑149 (MQ=255)                                                                     tCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCCGGCCTGACCGCCAGCGCCTTCtcctcc                                                                                                      <  1:190134/117‑1 (MQ=255)                                                                     tCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCtcctcc                                                                                                      >  2:190134/1‑116 (MQ=255)                                                                            aaCTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAAc                                                               >  3:263843/1‑148 (MQ=255)                                                                              gtGTCTACAAGCCGCTT‑CGGGGCGCCTCCCCGCCGGGCGTTACCGTCTTCCCCACCCCGCACCACGAGGGCTCGGTCGT‑GGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                            <  6:213968/148‑1 (MQ=25)                                                                              cTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                            <  4:263843/149‑1 (MQ=255)                                                                                    aCAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                      >  7:237189/1‑149 (MQ=255)                                                                                      aaGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGa                                                    <  2:211579/149‑1 (MQ=255)                                                                                          aCGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTc                                                 <  4:59035/148‑1 (MQ=255)                                                                                                           cccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                            >  2:99885/1‑121 (MQ=255)                                                                                                           cccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                            <  1:99885/121‑1 (MQ=255)                                                                                                            ccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTAGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATc                                 >  5:274793/1‑147 (MQ=255)                                                                                                              gTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAg                              >  3:319219/1‑148 (MQ=255)                                                                                                                   gCGTTACCGTCATCACCCCCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGCCCGCCAGCGCCTTCTCCTCCCTGTCGAGGGCCCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAg                        >  6:101842/1‑149 (MQ=255)                                                                                                                          cGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTg                 >  1:9127/1‑149 (MQ=255)                                                                                                                               tcaCCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt            <  8:237189/149‑1 (MQ=255)                                                                                                                               tcaCCACCCAGGACGACAACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt            >  5:271521/1‑149 (MQ=255)                                                                                                                                caccacCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt            >  8:242682/1‑148 (MQ=255)                                                                                                                                  ccaccCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAc         <  8:247234/149‑1 (MQ=255)                                                                                                                                   caccCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAcc        >  6:145532/1‑149 (MQ=255)                                                                                                                                      tcAGGCCGACGACCGAGCCGTCGT‑CGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCTAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgc     >  1:302090/2‑149 (MQ=37)                                                                                                                                         ggccgacgaccGAGCCGTCGT‑CGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGt  >  8:27803/4‑149 (MQ=37)                                                                                                                                                     |                                                                                                                                          TATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGT  >  NC_002947/4138438‑4138721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑