Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,138,596	G→T	9.5%	G32V (GGC→GTC)	PP_3643 →	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,138,596	0	G	T	9.5%	114.9 / 4.0	42	G32V (GGC→GTC)	PP_3643	oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (21/16);  new base T (2/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

ACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCAGGCCGAA  >  NC_002947/4138451‑4138741                                                                                                                                                    |                                                                                                                                                  acaTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGT‑CGTCCt                                                                                                                                                <  4:94627/149‑1 (MQ=255) acaTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCt                                                                                                                                                <  5:54829/149‑1 (MQ=255)   aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGa                                                                                                                                              >  6:349498/1‑149 (MQ=255)   aTCAACGATAAAAACCGCGGACCACCCCAGCCCTTCAGAGGTACTCCCGATGATCGAAGCAACTGTCTACAAGAACGTA‑CTGGGCTCCTTCCCCTCCGGCGAAACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGa                                                                                                                                              >  5:74159/1‑149 (MQ=255)   aTCAACGATAAAAACCGCGGACCACAGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGa                                                                                                                                              >  3:59035/1‑149 (MQ=255)      aaCGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGT‑CGTCCTgggtg                                                                                                                                           <  3:261079/149‑5 (MQ=255)               aaCCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCt                                                                                                                                  >  7:77697/1‑149 (MQ=255)                                          ggTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCcgccgc                                                                                                       <  8:77697/149‑1 (MQ=255)                                                 ccGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                                                >  8:234521/1‑149 (MQ=255)                                                 ccGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                                                >  7:247234/1‑149 (MQ=255)                                                 ccGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                                                >  5:213968/1‑149 (MQ=255)                                                   gatgatCGACGCAACTGTCTACAAGAACGTTGCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGCCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                                               >  1:211579/1‑149 (MQ=255)                                                        tCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCCGGCCTGACCGCCAGCGCCTTCtcctcc                                                                                                                          <  1:190134/117‑1 (MQ=255)                                                        tCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCtcctcc                                                                                                                          >  2:190134/1‑116 (MQ=255)                                                               aaCTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAAc                                                                                   >  3:263843/1‑148 (MQ=255)                                                                 gtGTCTACAAGCCGCTT‑CGGGGCGCCTCCCCGCCGGGCGTTACCGTCTTCCCCACCCCGCACCACGAGGGCTCGGTCGT‑GGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                                                <  6:213968/148‑1 (MQ=25)                                                                 cTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                                                <  4:263843/149‑1 (MQ=255)                                                                       aCAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                                          >  7:237189/1‑149 (MQ=255)                                                                         aaGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGa                                                                        <  2:211579/149‑1 (MQ=255)                                                                             aCGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTc                                                                     <  4:59035/148‑1 (MQ=255)                                                                                              cccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                                                <  1:99885/121‑1 (MQ=255)                                                                                              cccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                                                >  2:99885/1‑121 (MQ=255)                                                                                               ccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTAGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATc                                                     >  5:274793/1‑147 (MQ=255)                                                                                                 gTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAg                                                  >  3:319219/1‑148 (MQ=255)                                                                                                      gCGTTACCGTCATCACCCCCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGCCCGCCAGCGCCTTCTCCTCCCTGTCGAGGGCCCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAg                                            >  6:101842/1‑149 (MQ=255)                                                                                                             cGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTg                                     >  1:9127/1‑149 (MQ=255)                                                                                                                  tcaCCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt                                <  8:237189/149‑1 (MQ=255)                                                                                                                  tcaCCACCCAGGACGACAACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt                                >  5:271521/1‑149 (MQ=255)                                                                                                                   caccacCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt                                >  8:242682/1‑148 (MQ=255)                                                                                                                     ccaccCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAc                             <  8:247234/149‑1 (MQ=255)                                                                                                                      caccCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAcc                            >  6:145532/1‑149 (MQ=255)                                                                                                                         tcAGGCCGACGACCGAGCCGTCGT‑CGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCTAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgc                         >  1:302090/2‑149 (MQ=37)                                                                                                                            ggccgacgaccGAGCCGTCGT‑CGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGt                      >  8:27803/4‑149 (MQ=37)                                                                                                                                         tCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAAcag           >  2:120233/1‑147 (MQ=255)                                                                                                                                           ggTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTg                                                        >  4:92694/1‑100 (MQ=255)                                                                                                                                           ggTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTg                                                        <  3:92694/100‑1 (MQ=255)                                                                                                                                           ggTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTTCACCGGACAGCAgg       <  5:349498/149‑1 (MQ=37)                                                                                                                                           ggTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAAcagcag        <  7:242682/148‑1 (MQ=255)                                                                                                                                             tcgt‑cgGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGGTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGAAGGcc     >  2:62823/1‑149 (MQ=255)                                                                                                                                                t‑cgGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTTCACCGGACAGCAggtgaat  <  6:274793/149‑6 (MQ=37)                                                                                                                                                t‑cgGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCAGGCCGaa  <  7:234521/149‑1 (MQ=255)                                                                                                                                                t‑cgGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCAGGCCGaa  <  5:145532/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTT‑CTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGT‑CGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCAGGCCGAA  >  NC_002947/4138451‑4138741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑