Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,142,396 A→G 6.1% K177E (AAA→GAA) ‡ PP_3646 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,142,3960AG 6.1% 193.6 / 2.3 65K177E (AAA→GAA) ‡PP_3646aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base A (34/27);  new base G (2/2);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TTGGACAAACCCGACCTGTTCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGC  >  NC_002947/4142255‑4142539
                                                                                                                                             |                                                                                                                                               
ttGGACAAACCCGACCTGTTCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTc                                                                                                                                          >  7:185448/1‑149 (MQ=255)
    aCAAACCCGACCTGTTCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTcc                                                                                                                                         >  5:79761/1‑146 (MQ=255)
    aCAAACCCGACCTGTTCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGa                                                                                                                                       <  6:79761/148‑1 (MQ=255)
    aCAAACCCGACCTGTTCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCCGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAAACGTcc                                                                                                                                         >  5:74733/1‑146 (MQ=255)
    aCAAACCCGACCTGTTCGCATTCTCCGTGCAAGAGCAAATCGGCGTGGTTGCCGGGATCATCCCGTGTAACAGCCCTCTGTACCTCAACGCGATCAAGCTGGCGCGGGACTTGGCTGCCGGCAACACCATCGTGCTCATACCGTcc                                                                                                                                         <  6:74733/146‑1 (MQ=255)
          ccGACCTGTTCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCGGACGGTTTGAGCACgat                                                                                                                                <  7:136844/149‑3 (MQ=255)
            gACCTGTTCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTc                                                                                                                              <  3:282596/149‑1 (MQ=255)
                 gTTCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCaa                                                                                                                         >  6:132998/1‑149 (MQ=255)
                  ttCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGcc                                                                                                                                >  8:185477/1‑141 (MQ=255)
                  ttCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGcc                                                                                                                                <  7:190453/141‑1 (MQ=255)
                  ttCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGcc                                                                                                                                <  7:185477/141‑1 (MQ=255)
                  ttCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGcc                                                                                                                                >  8:190453/1‑141 (MQ=255)
                  ttCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAAc                                                                                                                        >  1:8803/1‑149 (MQ=255)
                       cTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATcc                                                                                                                   >  8:212845/1‑149 (MQ=255)
                        tACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCt                                                                                                                  >  6:306860/1‑149 (MQ=255)
                          cacCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTc                                                                                                                 >  1:290142/1‑148 (MQ=255)
                                 cACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTgg                                                                                                          <  8:185448/148‑1 (MQ=255)
                                    gAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGcc                                                                                                        <  6:291992/147‑1 (MQ=255)
                                    gAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGACTCGGCAACCATCCTCGAGCTGGccc                                                                                                       >  5:291992/1‑148 (MQ=255)
                                     aGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGa                                                                                                     <  7:82669/149‑1 (MQ=255)
                                      gCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGAc                                                                                                    <  2:290142/149‑1 (MQ=255)
                                          aTCGGCGTGGTGGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGc                                                                                                <  8:100248/149‑1 (MQ=255)
                                              gCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCtt                                                                                            <  5:132998/149‑1 (MQ=255)
                                                gTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGa                                                                                          <  3:189886/149‑1 (MQ=255)
                                                   gTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGc                                                                                       >  5:325036/1‑149 (MQ=255)
                                                        cGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCt                                                                                  >  7:142667/1‑149 (MQ=255)
                                                                    gTGGAACAGCCCGCTGTACCTCACCGCGATCATGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTTGGCAACCATCCTCGAGCTGGCCCGa                                                                                                     <  6:351104/118‑1 (MQ=255)
                                                                    gTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTTGGCAACCATCCTCGAGCTGGCCCGa                                                                                                     >  5:351104/1‑118 (MQ=255)
                                                                    gTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTccc                                                                              <  5:256668/141‑1 (MQ=255)
                                                                    gTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTccc                                                                              >  6:256668/1‑141 (MQ=255)
                                                                             cccGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACg                                                             >  2:51349/1‑149 (MQ=255)
                                                                             cccGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACg                                                             >  7:120971/1‑149 (MQ=255)
                                                                              ccGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGt                                                            >  5:186770/1‑149 (MQ=255)
                                                                              ccGCTGTACCTCACCGCGATCAAGCTGGCCCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGt                                                            <  5:306860/149‑1 (MQ=255)
                                                                                 cTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGt                                                         >  5:290290/1‑149 (MQ=255)
                                                                                 cTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGt                                                         >  2:342434/1‑149 (MQ=255)
                                                                                   gTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCa                                                       >  5:321379/1‑149 (MQ=255)
                                                                                      ccTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCg                                                    >  3:212803/1‑149 (MQ=255)
                                                                                       cTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCgg                                                   >  8:323831/1‑149 (MQ=255)
                                                                                               gATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGcc                                           <  6:325036/149‑1 (MQ=255)
                                                                                                aTCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGccc                                          <  8:142667/149‑1 (MQ=255)
                                                                                                 tCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGcccc                                         <  2:8803/149‑1 (MQ=255)
                                                                                                 tCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTACTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCgg                                                   >  4:143849/1‑139 (MQ=255)
                                                                                                 tCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTACTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCgg                                                   <  3:143849/139‑1 (MQ=255)
                                                                                                   aaGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCa                                        <  5:167612/148‑1 (MQ=255)
                                                                                                   aaGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAg                                       >  6:77413/1‑149 (MQ=255)
                                                                                                      cTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCAc                                    <  8:120971/149‑1 (MQ=255)
                                                                                                         gcgcCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACcgg                                 >  5:200743/1‑149 (MQ=255)
                                                                                                           gcCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACcggcg                               >  7:165392/1‑149 (MQ=255)
                                                                                                                ccTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACcggcgcggcg                          <  5:77413/149‑1 (MQ=255)
                                                                                                                 cTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCggcgcggcgc                         >  3:279441/1‑149 (MQ=255)
                                                                                                                      cTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGAcc                    >  5:59010/1‑149 (MQ=255)
                                                                                                                       tGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGAccc                   >  7:290542/1‑149 (MQ=255)
                                                                                                                              aaCACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCAccc            >  2:231273/1‑149 (MQ=255)
                                                                                                                               acacCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCg           <  6:186770/149‑1 (MQ=255)
                                                                                                                                cacCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGc          <  6:200743/149‑1 (MQ=255)
                                                                                                                                 cccATCGTGCTCAAACCGTCCGAGCACGCCTCGGTAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCCGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACACGCt         >  7:221583/2‑149 (MQ=255)
                                                                                                                                  ccATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTg        <  1:342434/149‑1 (MQ=255)
                                                                                                                                  ccATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTg        <  6:290290/149‑1 (MQ=255)
                                                                                                                                   cATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTgg       >  8:44511/1‑149 (MQ=255)
                                                                                                                                   cATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTgg       >  8:59174/1‑149 (MQ=255)
                                                                                                                                    ggcGTGCTCGGACGGTTTGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGt      >  6:43222/3‑149 (MQ=255)
                                                                                                                                    ggcGTGCTCGGACGGTTTGAGCACGCCTAGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGt      <  7:251232/147‑1 (MQ=255)
                                                                                                                                    ggcGTGCTCGGACGGTTTGAGCACGCCTAGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGt      >  8:251232/3‑149 (MQ=255)
                                                                                                                                        tGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTgcgc  <  4:279441/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
TTGGACAAACCCGACCTGTTCGCCTACACCGTGCACGAGCCAATCGGCGTGGTTGCCGGGATCATCCCGTGGAACAGCCCGCTGTACCTCACCGCGATCAAGCTGGCGCCGGCCTTGGCTGCCGGCAACACCATCGTGCTCAAACCGTCCGAGCACGCCTCGGCAACCATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCCGGCCGGTGTGGTCAACGTGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGC  >  NC_002947/4142255‑4142539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑