Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,143,883	T→A	11.3%	intergenic (+36/‑87)	PP_3647 → / → PP_3648	oxidoreductase/carboxymuconolactone decarboxylase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,143,883	0	T	A	11.3%	107.7 / 2.8	42	intergenic (+36/‑87)	PP_3647/PP_3648	oxidoreductase/carboxymuconolactone decarboxylase family protein
Reads supporting (aligned to +/- strand):  ref base T (21/16);  new base A (3/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCGATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTGGGCGAGGAC  >  NC_002947/4143741‑4144029                                                                                                                                               |                                                                                                                                                   aCGCGATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCAAGCCcgc                                                                                                                                              <  4:290733/149‑1 (MQ=255)     gATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAg                                                                                                                                          <  4:56900/149‑1 (MQ=255)       tCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCAAGCCCGCGAAGAt                                                                                                                                        <  1:162300/149‑1 (MQ=255)                      tGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAgg                                                                                                                         >  3:2092/1‑149 (MQ=255)                             ccTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGAt                                                                                                                                        <  8:246367/127‑1 (MQ=255)                             ccTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGAt                                                                                                                                        >  7:246367/1‑127 (MQ=255)                             ccTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGCCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTcgcg                                                                                                                  >  1:232736/1‑149 (MQ=255)                                 gtgtTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCCGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTaa                                                                                                              >  3:280591/1‑149 (MQ=255)                                  tgtTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGtttt                                                                                                                             >  8:115303/1‑133 (MQ=255)                                  tgtTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGtttt                                                                                                                             <  7:115303/133‑1 (MQ=255)                                      gCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGtc                                                                                                         >  1:175771/1‑149 (MQ=255)                                      gCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGtc                                                                                                         >  3:16528/1‑149 (MQ=255)                                       ccTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGCCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGtc                                                                                                         <  2:232736/148‑1 (MQ=255)                                                  gATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGtt                                                                                             >  1:263942/1‑149 (MQ=255)                                                        cccGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGcc                                                                                       >  4:222432/1‑149 (MQ=255)                                                         ccGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACACTCGGTTGCGGccc                                                                                      >  8:80558/1‑149 (MQ=255)                                                                 gtACCACAAGGTCAATCTCGTATACCTGACGCCACTTGGATGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCAGTTTTCAGGGGGCTCGCTAATCGACACACCCTCGGATGCGGCCCCAATTTTa                                                                              >  6:300293/1‑149 (MQ=255)                                                                    ccACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGc                                                                           <  3:222432/149‑1 (MQ=255)                                                                              aaGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGcccc                                                                                     >  3:90562/1‑129 (MQ=255)                                                                              aaGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGcccc                                                                                     <  4:90562/129‑1 (MQ=255)                                                                                  tCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGcaca                                                             >  7:274849/1‑149 (MQ=255)                                                                                    ggCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACAt                                                            >  7:299678/1‑148 (MQ=255)                                                                                           tGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAAt                                                     >  3:150315/1‑148 (MQ=255)                                                                                               gccACTTGGCTGATACATCTTGAGTCGTTGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGaaaa                                                >  1:14391/1‑149 (MQ=255)                                                                                                    ttGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACt                                                                  <  2:336300/126‑1 (MQ=255)                                                                                                    ttGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACt                                                                  >  1:336300/1‑126 (MQ=255)                                                                                                        cTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGaaaa                                       <  2:175771/149‑1 (MQ=255)                                                                                                         tGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAg                                      >  5:329294/1‑149 (MQ=255)                                                                                                             aCATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTAACCCTAGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATTAGCAATGAAAAGTACGAAAAGGGcc                                  >  2:299188/1‑149 (MQ=255)                                                                                                                    gAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCTAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGNCCTGa                               <  7:80558/145‑1 (MQ=255)                                                                                                                    gAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGaaaa                            <  2:14391/148‑1 (MQ=255)                                                                                                                    gAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGaaa                             <  4:280591/147‑1 (MQ=255)                                                                                                                     aGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCGCATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAAt                           <  2:263942/148‑1 (MQ=255)                                                                                                                     aGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATc                          >  1:95675/1‑149 (MQ=255)                                                                                                                         gATGTTGACATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCa                      >  6:95593/1‑149 (MQ=255)                                                                                                                                  aTCTTCGCGGGCAAGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTAc             >  5:219648/1‑149 (MQ=255)                                                                                                                                    cTTCGCGGGCAAGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAAACGTCTCACCCTCGGTTGCGGGCCCGTTTTTATGCCGGAGTTCTGCACAAGAGCAATGAAAAGTACGAAACGGGCCTGAAAATCCGCAGCCAgggtctg           >  4:27000/1‑144 (MQ=255)                                                                                                                                     ttCGCGGGCAAGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAACATCCGCACCCAGGTACTgg          >  3:5037/1‑149 (MQ=255)                                                                                                                                       cgcgGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTgg          <  5:780/147‑1 (MQ=255)                                                                                                                                        gcgGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTGGGCg       <  8:274849/149‑1 (MQ=255)                                                                                                                                         cgGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTGGGCGa      <  2:95675/149‑1 (MQ=255)                                                                                                                                            gCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTGGGCGAGGa   <  6:329294/149‑1 (MQ=255)                                                                                                                                              ttGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTGGGCGAGGAc  >  2:199068/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                                   ACGCGATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTGGGCGAGGAC  >  NC_002947/4143741‑4144029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑