Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,170,183	A→T	10.1%	intergenic (‑291/‑96)	katG ← / → PP_3669	catalase‑peroxidase/LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,170,183	0	A	T	10.1%	145.2 / 8.2	60	intergenic (‑291/‑96)	katG/PP_3669	catalase‑peroxidase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (28/24);  new base T (2/4);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01

GCAGGTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGTTC  >  NC_002947/4170035‑4170329                                                                                                                                                      |                                                                                                                                                   gCAGGTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCaa                                                                                                                                                    <  7:171220/149‑1 (MQ=255)   aGGTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGc                                                                                                                                                  <  5:319327/149‑1 (MQ=255)     gTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGccc                                                                                                                                                >  4:102871/1‑149 (MQ=255)        cTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCcgcg                                                                                                                                             <  3:104262/149‑1 (MQ=255)          gACGCGCTGCCCGGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTGCC‑GGCCTCATCGCGGGCTTGACCGCGAt                                                                                                                                           <  8:215914/149‑1 (MQ=255)           aCGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGGCTTGACCGCGATg                                                                                                                                          <  3:250618/149‑1 (MQ=255)             gcgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCCGTCAAGCCCACGATGAg                                                                                                                                        >  4:54924/1‑149 (MQ=255)              cgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAgg                                                                                                                                       <  4:42749/149‑1 (MQ=255)               gcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGc                                                                                                                                      <  5:47200/149‑1 (MQ=255)               gcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGc                                                                                                                                      <  5:42097/149‑1 (MQ=255)                  gCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCgg                                                                                                                                   >  7:107595/1‑149 (MQ=255)                   cccTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAACCCGCGAAGAGGcccgg                                                                                                                                  >  2:308165/1‑146 (MQ=255)                   cccTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGGCTTGACCGCGATGa                                                                                                                                         >  1:223071/1‑142 (MQ=255)                   cccTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGGCTTGACCGCGATGa                                                                                                                                         <  2:223071/142‑1 (MQ=255)                           cgGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCAt                                                                                                                          <  8:107595/149‑1 (MQ=255)                           cgGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCAt                                                                                                                          <  3:48604/149‑1 (MQ=255)                            ggATTTCGGGGGGCCACGAGAATGACTCGCCGCGGGTGGGCTGAGGTCTAATTGTGGGGCTGTTGGAGCCTGATTGGCGGGCTCGGGCTGGCGCTGGCCGGTATC‑GGCCTCATCGCGGTCACGCCCGCGATGTGGCCGGCACATCCATa                                                                                                                         <  2:307016/149‑1 (MQ=255)                            ggAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGGCCAGCCCGCGATGCGGCCGGCCCCTCCCTa                                                                                                                         >  1:307016/1‑149 (MQ=255)                                        gCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaaggcacaagg                                                                                                             >  6:338766/1‑149 (MQ=255)                                                aaaGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCgcgc                                                                                                     >  6:129878/1‑149 (MQ=255)                                                    aCTCGCCGTGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCGGTATC‑GCCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATg                                                                                                 <  1:308165/149‑1 (MQ=255)                                                    aCTCGCCGCGCTTGGGCAGAGGTCTAATAGCGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGGCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATg                                                                                                 <  7:303386/149‑1 (MQ=255)                                                    aCTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATg                                                                                                 <  3:102871/149‑1 (MQ=255)                                                       aGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTAAAAGCCGGGAACACCGCGAAGAGGCCGCGACCAACATACAAGGCCCAACACCCCCCCCCaacga                                                                                               >  8:119364/1‑144 (MQ=255)                                                        gCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaaggcacaagg                                                                                                             <  3:320202/133‑1 (MQ=255)                                                        gCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaaggcacaagg                                                                                                             >  4:320202/1‑133 (MQ=255)                                                        gCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGGCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCtt                                                                                              >  5:349872/1‑148 (MQ=255)                                                         ccGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGGCCTAAACGCGGGAAAGCCCGCCATGAGGCCGGCCACTCCAAAAAAGGCCAAAGGCCCCGCCCCAAGCtt                                                                                              >  5:215734/1‑148 (MQ=255)                                                               ttGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATaca                                                                                       >  3:124696/1‑148 (MQ=255)                                                                 gggCAGAGGTCTAATAGAGTGGATGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATAGCTGAAAACCCCCCGATGAGGCCGGCACATACATACAAGGCAAAATGACCCGCGCCATGCTTAAATACACAg                                                                                    >  4:140082/1‑149 (MQ=255)                                                                         gTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAGGGCCCCGCGCCATGCTTCAATACACAGCCATAccc                                                                            >  3:82786/1‑149 (MQ=255)                                                                           cTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCtt                                                                          >  2:258943/1‑149 (MQ=255)                                                                            tAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCttt                                                                         >  5:83080/1‑149 (MQ=255)                                                                               tAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGcc                                                                      >  4:312892/1‑149 (MQ=255)                                                                                   gTGGCTGTTGGAGCCTGATTGGCTGGCTGTTTCCGGCGCAGGCCTTTATC‑GGCATCATCGCGGTCACGCCCGCTTTGCGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAg                                                                   <  7:119364/148‑1 (MQ=255)                                                                                     ggCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGAc                                                                >  4:263233/1‑149 (MQ=255)                                                                                     ggCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATAGCCGTCAACCCCGCGATGGGGCCGGGCCCTCCATAAAAGGCACAAGGCCCCGGGCCATTCTTGAAAAAACCGCCATTCCCGTTGgccctgac                                                                >  4:188523/1‑144 (MQ=255)                                                                                          ttGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATGCAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGa                                                           >  4:309469/1‑149 (MQ=255)                                                                                               gCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACATGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCt                                                      <  4:82786/149‑1 (MQ=255)                                                                                                 cTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCa                                                    <  5:338766/149‑1 (MQ=255)                                                                                                    aTAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTc                                                     <  5:253289/145‑1 (MQ=255)                                                                                                    aTAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTc                                                     >  6:253289/1‑145 (MQ=255)                                                                                                       ggctggctCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAAc                                               <  5:129878/148‑1 (MQ=255)                                                                                                        gctggctCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATAGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCg                                             >  5:201966/1‑149 (MQ=255)                                                                                                           ggcgCTGTCAGGCGCAGGCCTTTATC‑GGCCTCATCGCGGTCAAGCCCGCTATTAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCaa                                          <  3:54924/149‑1 (MQ=255)                                                                                                            gctCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAAc                                         <  4:124696/149‑1 (MQ=255)                                                                                                                  tCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCt                                    <  6:83080/148‑1 (MQ=255)                                                                                                                    aGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTgcg                                 >  5:280479/1‑149 (MQ=255)                                                                                                                       cgcAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGcgccg                              >  2:23111/1‑149 (MQ=255)                                                                                                                              cTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATGCAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGAc                       <  3:309469/149‑1 (MQ=255)                                                                                                                                 tATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACa                      <  3:312892/147‑1 (MQ=255)                                                                                                                                         cTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACctgc             >  2:317529/1‑149 (MQ=255)                                                                                                                                         cTCATCGCGGGCTTGACCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATAc                                                                              <  6:258809/84‑1 (MQ=37)                                                                                                                                         cTCATCGCGGGCTTGACCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATAc                                                                              >  5:258809/1‑84 (MQ=37)                                                                                                                                             tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTc                                                     <  3:187396/105‑1 (MQ=255)                                                                                                                                             tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTc                                                     >  4:187396/1‑105 (MQ=255)                                                                                                                                             tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGCCCTGCGCCGCGTCGACATGAACCTGCTGGt         >  7:76363/1‑149 (MQ=255)                                                                                                                                             tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACctgctg           <  3:300056/147‑1 (MQ=255)                                                                                                                                             tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACctgctg           >  4:300056/1‑147 (MQ=255)                                                                                                                                                  gTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCTACATGAACCTGCTGGTGCTGt    <  3:140082/149‑1 (MQ=255)                                                                                                                                                   tCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGtt   <  6:349872/149‑1 (MQ=255)                                                                                                                                                     aaGCCCGCGTTGAGGCCTGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGt    <  7:215062/146‑1 (MQ=255)                                                                                                                                                     aaGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTACAAAACACAGCCAAACACTTTGCCCAGGAAGCCGCGCCATCATGAACCCCAACGAACTGCGCCCCTGAGAAATGAACATGATGGTGCTGtaa  >  8:215062/1‑146 (MQ=255)                                                                                                                                                      |                                                                                                                                                   GCAGGTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGTTC  >  NC_002947/4170035‑4170329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑