Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,170,185	C→A	11.2%	intergenic (‑293/‑94)	katG ← / → PP_3669	catalase‑peroxidase/LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,170,185	0	C	A	11.2%	175.2 / 10.0	60	intergenic (‑293/‑94)	katG/PP_3669	catalase‑peroxidase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (27/26);  new base A (3/4);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

AGGTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGTTCGAAG  >  NC_002947/4170037‑4170333                                                                                                                                                      |                                                                                                                                                     aGGTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGc                                                                                                                                                      <  5:319327/149‑1 (MQ=255)   gTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGccc                                                                                                                                                    >  4:102871/1‑149 (MQ=255)      cTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCcgcg                                                                                                                                                 <  3:104262/149‑1 (MQ=255)        gACGCGCTGCCCGGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTGCC‑GGCCTCATCGCGGGCTTGACCGCGAt                                                                                                                                               <  8:215914/149‑1 (MQ=255)         aCGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGGCTTGACCGCGATg                                                                                                                                              <  3:250618/149‑1 (MQ=255)           gcgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCCGTCAAGCCCACGATGAg                                                                                                                                            >  4:54924/1‑149 (MQ=255)            cgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAgg                                                                                                                                           <  4:42749/149‑1 (MQ=255)             gcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGc                                                                                                                                          <  5:47200/149‑1 (MQ=255)             gcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGc                                                                                                                                          <  5:42097/149‑1 (MQ=255)                gCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCgg                                                                                                                                       >  7:107595/1‑149 (MQ=255)                 cccTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAACCCGCGAAGAGGcccgg                                                                                                                                      >  2:308165/1‑146 (MQ=255)                 cccTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGGCTTGACCGCGATGa                                                                                                                                             >  1:223071/1‑142 (MQ=255)                 cccTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGGCTTGACCGCGATGa                                                                                                                                             <  2:223071/142‑1 (MQ=255)                         cgGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCAt                                                                                                                              <  8:107595/149‑1 (MQ=255)                         cgGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCAt                                                                                                                              <  3:48604/149‑1 (MQ=255)                          ggATTTCGGGGGGCCACGAGAATGACTCGCCGCGGGTGGGCTGAGGTCTAATTGTGGGGCTGTTGGAGCCTGATTGGCGGGCTCGGGCTGGCGCTGGCCGGTATC‑GGCCTCATCGCGGTCACGCCCGCGATGTGGCCGGCACATCCATa                                                                                                                             <  2:307016/149‑1 (MQ=255)                          ggAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGGCCAGCCCGCGATGCGGCCGGCCCCTCCCTa                                                                                                                             >  1:307016/1‑149 (MQ=255)                                      gCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaaggcacaagg                                                                                                                 >  6:338766/1‑149 (MQ=255)                                              aaaGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCgcgc                                                                                                         >  6:129878/1‑149 (MQ=255)                                                  aCTCGCCGTGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCGGTATC‑GCCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATg                                                                                                     <  1:308165/149‑1 (MQ=255)                                                  aCTCGCCGCGCTTGGGCAGAGGTCTAATAGCGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGGCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATg                                                                                                     <  7:303386/149‑1 (MQ=255)                                                  aCTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATg                                                                                                     <  3:102871/149‑1 (MQ=255)                                                     aGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTAAAAGCCGGGAACACCGCGAAGAGGCCGCGACCAACATACAAGGCCCAACACCCCCCCCCaacga                                                                                                   >  8:119364/1‑144 (MQ=255)                                                      gCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaaggcacaagg                                                                                                                 <  3:320202/133‑1 (MQ=255)                                                      gCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaaggcacaagg                                                                                                                 >  4:320202/1‑133 (MQ=255)                                                      gCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGGCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCtt                                                                                                  >  5:349872/1‑148 (MQ=255)                                                       ccGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGGCCTAAACGCGGGAAAGCCCGCCATGAGGCCGGCCACTCCAAAAAAGGCCAAAGGCCCCGCCCCAAGCtt                                                                                                  >  5:215734/1‑148 (MQ=255)                                                             ttGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATaca                                                                                           >  3:124696/1‑148 (MQ=255)                                                               gggCAGAGGTCTAATAGAGTGGATGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATAGCTGAAAACCCCCCGATGAGGCCGGCACATACATACAAGGCAAAATGACCCGCGCCATGCTTAAATACACAg                                                                                        >  4:140082/1‑149 (MQ=255)                                                                       gTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAGGGCCCCGCGCCATGCTTCAATACACAGCCATAccc                                                                                >  3:82786/1‑149 (MQ=255)                                                                         cTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCtt                                                                              >  2:258943/1‑149 (MQ=255)                                                                          tAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCttt                                                                             >  5:83080/1‑149 (MQ=255)                                                                             tAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGcc                                                                          >  4:312892/1‑149 (MQ=255)                                                                                 gTGGCTGTTGGAGCCTGATTGGCTGGCTGTTTCCGGCGCAGGCCTTTATC‑GGCATCATCGCGGTCACGCCCGCTTTGCGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAg                                                                       <  7:119364/148‑1 (MQ=255)                                                                                   ggCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGAc                                                                    >  4:263233/1‑149 (MQ=255)                                                                                   ggCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATAGCCGTCAACCCCGCGATGGGGCCGGGCCCTCCATAAAAGGCACAAGGCCCCGGGCCATTCTTGAAAAAACCGCCATTCCCGTTGgccctgac                                                                    >  4:188523/1‑144 (MQ=255)                                                                                        ttGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATGCAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGa                                                               >  4:309469/1‑149 (MQ=255)                                                                                             gCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACATGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCt                                                          <  4:82786/149‑1 (MQ=255)                                                                                               cTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCa                                                        <  5:338766/149‑1 (MQ=255)                                                                                                  aTAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTc                                                         <  5:253289/145‑1 (MQ=255)                                                                                                  aTAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTc                                                         >  6:253289/1‑145 (MQ=255)                                                                                                     ggctggctCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAAc                                                   <  5:129878/148‑1 (MQ=255)                                                                                                      gctggctCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATAGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCg                                                 >  5:201966/1‑149 (MQ=255)                                                                                                         ggcgCTGTCAGGCGCAGGCCTTTATC‑GGCCTCATCGCGGTCAAGCCCGCTATTAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCaa                                              <  3:54924/149‑1 (MQ=255)                                                                                                          gctCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAAc                                             <  4:124696/149‑1 (MQ=255)                                                                                                                tCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCt                                        <  6:83080/148‑1 (MQ=255)                                                                                                                  aGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTgcg                                     >  5:280479/1‑149 (MQ=255)                                                                                                                     cgcAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGcgccg                                  >  2:23111/1‑149 (MQ=255)                                                                                                                            cTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATGCAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGAc                           <  3:309469/149‑1 (MQ=255)                                                                                                                               tATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACa                          <  3:312892/147‑1 (MQ=255)                                                                                                                                       cTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACctgc                 >  2:317529/1‑149 (MQ=255)                                                                                                                                       cTCATCGCGGGCTTGACCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATAc                                                                                  <  6:258809/84‑1 (MQ=37)                                                                                                                                       cTCATCGCGGGCTTGACCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATAc                                                                                  >  5:258809/1‑84 (MQ=37)                                                                                                                                           tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTc                                                         >  4:187396/1‑105 (MQ=255)                                                                                                                                           tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTc                                                         <  3:187396/105‑1 (MQ=255)                                                                                                                                           tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGCCCTGCGCCGCGTCGACATGAACCTGCTGGt             >  7:76363/1‑149 (MQ=255)                                                                                                                                           tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACctgctg               >  4:300056/1‑147 (MQ=255)                                                                                                                                           tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACctgctg               <  3:300056/147‑1 (MQ=255)                                                                                                                                                gTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCTACATGAACCTGCTGGTGCTGt        <  3:140082/149‑1 (MQ=255)                                                                                                                                                 tCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGtt       <  6:349872/149‑1 (MQ=255)                                                                                                                                                   aaGCCCGCGTTGAGGCCTGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGt        <  7:215062/146‑1 (MQ=255)                                                                                                                                                   aaGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTACAAAACACAGCCAAACACTTTGCCCAGGAAGCCGCGCCATCATGAACCCCAACGAACTGCGCCCCTGAGAAATGAACATGATGGTGCTGtaa      >  8:215062/1‑146 (MQ=255)                                                                                                                                                      cccGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGTTCGAAg  >  5:91173/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     AGGTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATC‑GGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGTTCGAAG  >  NC_002947/4170037‑4170333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑